{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T23:26:20Z","timestamp":1777073180791,"version":"3.51.4"},"reference-count":59,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2023,5,6]],"date-time":"2023-05-06T00:00:00Z","timestamp":1683331200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Italian Ministry of Health-Ricerca Corrente degli IRCCS, linea 1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>Despite the number of cholera outbreaks reported worldwide, only a few cases are recorded among returning European travellers. We describe the case of a 41-year-old male, returning to Italy after a stay in Bangladesh, his origin country, who presented with watery diarrhoea. Vibrio cholerae and norovirus were detected in the patient\u2019s stools via multiplex PCR methods. Direct microscopy, Gram staining, culture and antibiotic susceptibility tests were performed. The isolates were tested using end-point PCR for the detection of potentially enteropathogenic V. cholera. Serotype and cholera toxins identification were carried out. Whole genome sequencing and bioinformatics analysis were performed, and antimicrobial resistance genes identified. A phylogenetic tree with the most similar genomes of databases previously described was built. Sample of the food brought back by the patient were also collected and analysed. The patient was diagnosed with V. cholerae O1, serotype Inaba, norovirus and SARS-CoV-2 concomitant infection. The isolated V. cholerae strain was found to belong to ST69, encoding for cholera toxin, ctxB7 type and was phylogenetically related to the 2018 outbreak in Dhaka, Bangladesh. Adopting a multidisciplinary approach in a cholera non-endemic country ensured rapid and accurate diagnosis, timely clinical management, and epidemiological investigation at national and international level.<\/jats:p>","DOI":"10.3390\/tropicalmed8050266","type":"journal-article","created":{"date-parts":[[2023,5,8]],"date-time":"2023-05-08T02:03:31Z","timestamp":1683511411000},"page":"266","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A Cholera Case Imported from Bangladesh to Italy: Clinico-Epidemiological Management and Molecular Characterization in a Non-Endemic Country"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5127-5686","authenticated-orcid":false,"given":"Valeria","family":"Russini","sequence":"first","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale del Lazio e della Toscana \u201cM. Aleandri\u201d\u2014Sezione di Roma, 00178 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2481-6621","authenticated-orcid":false,"given":"Maria Letizia","family":"Giancola","sequence":"additional","affiliation":[{"name":"National Institute for Infectious Diseases (INMI) \u201cLazzaro Spallanzani\u201d, IRCCS, 00149 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grazia","family":"Brunetti","sequence":"additional","affiliation":[{"name":"Policlinico Casilino General Hospital, 00169 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmela","family":"Calbi","sequence":"additional","affiliation":[{"name":"Policlinico Casilino General Hospital, 00169 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Anzivino","sequence":"additional","affiliation":[{"name":"Policlinico Casilino General Hospital, 00169 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5369-0437","authenticated-orcid":false,"given":"Carla","family":"Nisii","sequence":"additional","affiliation":[{"name":"National Institute for Infectious Diseases (INMI) \u201cLazzaro Spallanzani\u201d, IRCCS, 00149 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucia","family":"Scaramella","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale del Lazio e della Toscana \u201cM. Aleandri\u201d\u2014Sezione di Roma, 00178 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna Maria","family":"Dionisi","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Istituto Superiore di Sanit\u00e0, 00161 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesca","family":"Faraglia","sequence":"additional","affiliation":[{"name":"National Institute for Infectious Diseases (INMI) \u201cLazzaro Spallanzani\u201d, IRCCS, 00149 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9131-7502","authenticated-orcid":false,"given":"Marina","family":"Selleri","sequence":"additional","affiliation":[{"name":"National Institute for Infectious Diseases (INMI) \u201cLazzaro Spallanzani\u201d, IRCCS, 00149 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7380-7097","authenticated-orcid":false,"given":"Laura","family":"Villa","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Istituto Superiore di Sanit\u00e0, 00161 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah","family":"Lovari","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale del Lazio e della Toscana \u201cM. Aleandri\u201d\u2014Sezione di Roma, 00178 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3720-6550","authenticated-orcid":false,"given":"Maria Laura","family":"De Marchis","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale del Lazio e della Toscana \u201cM. Aleandri\u201d\u2014Sezione di Roma, 00178 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1663-0889","authenticated-orcid":false,"given":"Teresa","family":"Boss\u00f9","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale del Lazio e della Toscana \u201cM. 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