{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T22:49:01Z","timestamp":1788907741207,"version":"build-2803163510"},"reference-count":50,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,6,2]],"date-time":"2023-06-02T00:00:00Z","timestamp":1685664000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET) of Argentina","award":["PUE 2292180100054CO"],"award-info":[{"award-number":["PUE 2292180100054CO"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>The protoscolex (PSC) is generated by asexual reproduction at the larval stage of taeniid Echinococcus granulosus that causes cystic echinococcosis or hydatidosis, a worldwide zoonosis. The PSC is enveloped by a complex cellular syncytial tegument responsible for ionic movements and the hydroelectrolytic balance of the parasite. We recently reported on two electrical potentials in bovine lung protoscoleces (PSCs) that reflect differences in ionic movements between the parasite\u2019s invaginated and evaginated developmental stages. Here, we explored the effect of temperature and ionic substitutions on the tegumental potentials of bovine lung PSCs of Echinococcus granulosus by microelectrode impalements. We observed that the transient peak potential was temperature-dependent, consistent with an active transport component in the invaginated state only. Further changes in the electrical potentials by high K+ depolarization, low external Ca2+, and addition of the diuretic amiloride are in agreement with the presence of a Ca2+-sensitive cation-selective electrodiffusional pathway in the outer surface of the parasite. Variations in electrical potential differences through the tegument provide an accessible and valuable parameter for studying ionic transport mechanisms and, therefore, potential targets for developing novel antiparasitic drugs.<\/jats:p>","DOI":"10.3390\/tropicalmed8060303","type":"journal-article","created":{"date-parts":[[2023,6,2]],"date-time":"2023-06-02T08:50:31Z","timestamp":1685695831000},"page":"303","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Effect of Temperature and Ionic Substitutions on the Tegumental Potentials of Protoscoleces of Echinococcus granulosus"],"prefix":"10.3390","volume":"8","author":[{"given":"M\u00f3nica Patricia Antonella","family":"Carabajal","sequence":"first","affiliation":[{"name":"Laboratorio de Canales I\u00f3nicos, Instituto Multidisciplinario de Salud, Tecnolog\u00eda y Desarrollo (IMSaTeD, CONICET-UNSE), Santiago del Estero 4206, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Jos\u00e9","family":"Fern\u00e1ndez Salom","sequence":"additional","affiliation":[{"name":"Laboratorio de Canales I\u00f3nicos, Instituto Multidisciplinario de Salud, Tecnolog\u00eda y Desarrollo (IMSaTeD, CONICET-UNSE), Santiago del Estero 4206, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Santiago","family":"Olivera","sequence":"additional","affiliation":[{"name":"Laboratorio de Canales I\u00f3nicos, Instituto Multidisciplinario de Salud, Tecnolog\u00eda y Desarrollo (IMSaTeD, CONICET-UNSE), Santiago del Estero 4206, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7973-0804","authenticated-orcid":false,"given":"Horacio F.","family":"Cantiello","sequence":"additional","affiliation":[{"name":"Laboratorio de Canales I\u00f3nicos, Instituto Multidisciplinario de Salud, Tecnolog\u00eda y Desarrollo (IMSaTeD, CONICET-UNSE), Santiago del Estero 4206, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,2]]},"reference":[{"key":"ref_1","unstructured":"Thompson, R.C.A., and Lymbery, A.J. (2017). Advances in Parasitology, Academic Press. Chapter 2."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.ijid.2008.03.037","article-title":"Echinococcosis: A review","volume":"13","author":"Moro","year":"2009","journal-title":"Int. J. Infect. Dis."},{"key":"ref_3","unstructured":"Larrieu, E. (2017). Prevenci\u00f3n y Control de la Hidatidosis en el Nivel Local: Iniciativa Sudamericana para el Control y Vigilancia de la Equinococosis Qu\u00edstica\/Hidatidosis, Organizaci\u00f3n Panamericana de la Salud\u2014OPS\/OMS."},{"key":"ref_4","first-page":"e42","article-title":"Cystic echinococcosis in South America: A call for action","volume":"41","author":"Pavletic","year":"2017","journal-title":"Rev. Panam. Salud P\u00fablica Pan Am. J. Public Health"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1111\/tmi.12647","article-title":"Cystic echinococcosis in south America: Systematic review of species and genotypes of Echinococcus granulosus sensu lato in humans and natural domestic hosts","volume":"21","author":"Cucher","year":"2016","journal-title":"Trop. Med. Int. Health"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13071-021-04753-y","article-title":"Prevalence of human cystic echinococcosis in the towns of \u00d1orquinco and Ramos Mexia in Rio Negro province, Argentina, and direct risk factors for infection","volume":"14","author":"Uchiumi","year":"2021","journal-title":"Parasites Vectors"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"118","DOI":"10.4103\/2229-5070.162523","article-title":"Pulmonary hydatidosis from southern argentina","volume":"5","author":"Menghi","year":"2015","journal-title":"Trop. Parasitol."},{"key":"ref_8","unstructured":"Thompson, D.P., and Geary, T.G. (2003). Molecular Medical Parasitology, Academic Press."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1007\/s004360100435","article-title":"Depolarization of the tegument precedes morphological alterations in Echinococcus granulosus protoscoleces incubated with ivermectin","volume":"87","author":"Grosman","year":"2001","journal-title":"Parasitol. Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1006\/expr.1995.1148","article-title":"Echinococcus granulosus: Partial characterization of the conductive properties of two cation channels from protoscoleces of the ovine strain, reconstituted on planar lipid bilayers","volume":"81","author":"Grosman","year":"1995","journal-title":"Exp. Parasitol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1007\/s002329900246","article-title":"Interconverting gating modes of a nonselective cation channel from the tapeworm Echinococcus granulosus reconstituted on planar lipid bilayers","volume":"158","author":"Grosman","year":"1997","journal-title":"J. Membr. Biol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1007\/s12639-020-01224-6","article-title":"Study on the effect of an ion channel inhibitor \u201cfluralaner\u201d on Echinococcus granulosus protoscolices and metacestode layers in vitro","volume":"44","author":"Zahran","year":"2020","journal-title":"J. Parasit. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1038\/nature12031","article-title":"The genomes of four tapeworm species reveal adaptations to parasitism","volume":"496","author":"Tsai","year":"2013","journal-title":"Nature"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.ijpddr.2021.12.001","article-title":"Advances in our understanding of nematode ion channels as potential anthelmintic targets","volume":"18","author":"Choudhary","year":"2022","journal-title":"Int. J. Parasitol. Drugs Drug Resist."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1017\/S0031182099005715","article-title":"Electrophysiological investigation of anthelmintic resistance","volume":"120","author":"Martin","year":"2000","journal-title":"Parasitology"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/0014-4894(80)90071-5","article-title":"Schistosoma mansoni: Characterization of the electrical potential from the tegument of adult males","volume":"49","author":"Fetterer","year":"1980","journal-title":"Exp. Parasitol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1006\/expr.1994.1044","article-title":"Echinococcus granulosus: Characterization of the electrical potential of the syncytial tegument of protoscoleces incubated in vitro-effect of inhibitors","volume":"78","author":"Ibarra","year":"1994","journal-title":"Exp. Parasitol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/0014-4894(92)90211-R","article-title":"Echinococcus granulosus: Study of the in vitro complement activation by protoscoleces by measuring the electric potential difference across the tegumental membrane","volume":"75","author":"Ferreira","year":"1992","journal-title":"Exp. Parasitol."},{"key":"ref_19","first-page":"442","article-title":"Mecanismos de acci\u00f3n del mebendazol sobre el Echinococcus granulosus en su estad\u00edo larval estudiado in vitro","volume":"28","author":"Reisin","year":"1988","journal-title":"Bases para la interpretaci\u00f3n de los resultados de la quimioterapia experimental y cl\u00ednica Arch Intl Hidatid."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"108282","DOI":"10.1016\/j.exppara.2022.108282","article-title":"Electrical potentials of protoscoleces of the cestode Echinococcus granulosus from bovine origin","volume":"238","author":"Carabajal","year":"2022","journal-title":"Exp. Parasitol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/0005-2736(76)90512-5","article-title":"Temperature dependence of membrane function. The disparity between active potassium transport and (Na+ + K+) ATPase activity","volume":"443","author":"Ellory","year":"1976","journal-title":"Biochim. Biophys. Acta (BBA) Biomembr."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"11780","DOI":"10.1016\/S0021-9258(17)44298-0","article-title":"Brain (Na+,K+)-ATPase. Opposite effects of ethanol and dimethyl sulfoxide on temperature dependence of enzyme conformation and univalent cation binding","volume":"258","author":"Swann","year":"1983","journal-title":"J. Biol. Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"S10","DOI":"10.1152\/advances.1994.266.6.S10","article-title":"Demonstration of temperature dependence of Na+-K+ pump activity of human blood cells","volume":"266","author":"Honig","year":"1994","journal-title":"Adv. Physiol. Educ."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/0005-2736(91)90048-D","article-title":"The effect of cytoplasmic K+ on the activity of the Na+\/K+-ATPase","volume":"1067","author":"Cornelius","year":"1991","journal-title":"Biochim. Biophys. Acta (BBA) Biomembr."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1023\/A:1005580425932","article-title":"Contraction of epithelial (MDCK) cells in response to low extracellular calcium is dependent on extracellular sodium","volume":"20","author":"Lagunes","year":"1999","journal-title":"J. Muscle Res. Cell Motil."},{"key":"ref_26","first-page":"1009","article-title":"Role of the endoplasmic reticulum in shaping calcium dynamics in human lens cells","volume":"42","author":"Williams","year":"2001","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/BF01871102","article-title":"Amiloride and its analogs as tools in the study of ion transport","volume":"105","author":"Kleyman","year":"1988","journal-title":"J. Membr. Biol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1016\/0076-6879(90)91045-8","article-title":"Cation transport probes: The amiloride series","volume":"191","author":"Kleyman","year":"1990","journal-title":"Methods Enzymol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1007\/s40588-014-0007-6","article-title":"Ion channels and drug transporters as targets for anthelmintics","volume":"1","author":"Greenberg","year":"2014","journal-title":"Curr. Clin. Microbiol. Rep."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/0014-4894(77)90033-9","article-title":"Schistosoma mansoni: Direct method for simultaneous recording of electrical and motor activity","volume":"43","author":"Fetterer","year":"1977","journal-title":"Exp. Parasitol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1017\/S0031182000041895","article-title":"Na+-K+ transport, motility and tegumental membrane potential in adult male Schistosoma mansoni","volume":"82","author":"Fetterer","year":"1981","journal-title":"Parasitology"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1017\/S0031182000054226","article-title":"Microelectrode studies of the tegument and sub-tegumental compartments of male Schistosoma mansoni: Anatomical location of sources of electrical potentials","volume":"85","author":"Bricker","year":"1982","journal-title":"Parasitology"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13071-016-1428-2","article-title":"Pipeline for the identification and classification of ion channels in parasitic flatworms","volume":"9","author":"Nor","year":"2016","journal-title":"Parasites Vectors"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Jimenez, V., and Docampo, R. (2012). Molecular and electrophysiological characterization of a novel cation channel of Trypanosoma cruzi. PLoS Pathog., 8.","DOI":"10.1371\/journal.ppat.1002750"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1017\/S0031182000054238","article-title":"Microelectrode studies of the tegument and sub-tegumental compartments of male Schistosoma mansoni: An analysis of electrophysiological properties","volume":"85","author":"Thompson","year":"1982","journal-title":"Parasitology"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"C175","DOI":"10.1152\/ajpcell.1986.250.2.C175","article-title":"The amiloride-sensitive sodium channel","volume":"250","author":"Benos","year":"1986","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"ref_37","first-page":"546","article-title":"Mechanism of cardiac T-type Ca2+ channel blockade by amiloride","volume":"254","author":"Tytgat","year":"1990","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"C762","DOI":"10.1152\/ajpcell.1995.268.3.C762","article-title":"Amiloride-sensitive and HCO3(-)-dependent ion transport activated by aldosterone and vasotocin in A6 cells","volume":"268","author":"Doi","year":"1995","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1254\/jjp.68.279","article-title":"Nonselective effects of amiloride and its analogs on ion transport systems and their cytotoxicities in cardiac myocytes","volume":"68","author":"Murata","year":"1995","journal-title":"Jpn. J. Pharmacol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1007\/s002320001104","article-title":"Apical nonspecific cation channels in everted collecting tubules of potassium-adapted Ambystoma","volume":"177","author":"Stoner","year":"2000","journal-title":"J. Membr. Biol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1445","DOI":"10.1016\/S0169-409X(02)00161-8","article-title":"Altering airway surface liquid volume: Inhalation therapy with amiloride and hyperosmotic agents","volume":"54","author":"Hirsh","year":"2002","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_42","unstructured":"Cantiello, H., Ibarra, C., and Reisin, I. (1980). IX Reuni\u00f3n Anual de la Sociedad Argentina de Biof\u00edsica, Bermejo."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.vetpar.2015.07.031","article-title":"Recent advances in echinococcus genomics and stem cell research","volume":"213","author":"Koziol","year":"2015","journal-title":"Vet. Parasitol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/bs.apar.2016.09.006","article-title":"The echinococcoses: Diagnosis, clinical management and burden of disease","volume":"96","author":"Kern","year":"2017","journal-title":"Adv. Parasitol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/0169-4758(90)90227-U","article-title":"Mode of action of benzimidazoles","volume":"6","author":"Lacey","year":"1990","journal-title":"Parasitol. Today"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1097\/QCO.0b013e328318978f","article-title":"Praziquantel: Mechanisms of action, resistance and new derivatives for schistosomiasis","volume":"21","author":"Doenhoff","year":"2008","journal-title":"Curr. Opin. Infect. Dis."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1501","DOI":"10.1007\/s00436-011-2670-3","article-title":"New insight into praziquantel against various developmental stages of schistosomes","volume":"109","author":"Wu","year":"2011","journal-title":"Parasitol. Res."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Prole, D.L., and Taylor, C.W. (2011). Identification of intracellular and plasma membrane calcium channel homologs in pathogenic parasites. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0026218"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"C1277","DOI":"10.1152\/ajpcell.1999.277.6.C1277","article-title":"Role of gelsolin in the actin filament regulation of cardiac L-type calcium channels","volume":"277","author":"Lader","year":"1999","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"ref_50","unstructured":"Dur\u00e1n, M.A., Olivera, S.R., and Cantiello, H.F. (2018). XXXIII Jornadas Nacionales de Hidatidosis, Asociaci\u00f3n Parasitol\u00f3gica Argentina."}],"container-title":["Tropical Medicine and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2414-6366\/8\/6\/303\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:47:56Z","timestamp":1760125676000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2414-6366\/8\/6\/303"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,2]]},"references-count":50,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2023,6]]}},"alternative-id":["tropicalmed8060303"],"URL":"https:\/\/doi.org\/10.3390\/tropicalmed8060303","relation":{},"ISSN":["2414-6366"],"issn-type":[{"value":"2414-6366","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,2]]}}}