{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T07:10:30Z","timestamp":1780729830796,"version":"3.54.1"},"reference-count":74,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2023,9,6]],"date-time":"2023-09-06T00:00:00Z","timestamp":1693958400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>Leptospirosis has been a neglected, widespread and reemerging zoonotic disease of global importance. The One Health holistic approach combining human, animal and environmental health has been important for control and prevention of zoonotic disease. An urban municipality in Southern Brazil with a high prevalence of leptospirosis was selected by convenience, with asymptomatic human and canine individuals serologically tested using MAT against 30 Leptospira spp. serovars. Epidemiological questionnaires were assessed along with the historical national database, with associated risk factors to dog and human seropositivity analyzed using multiple logistic regression. The spatial distribution of retrospective human leptospirosis cases was analyzed using a Kernel map and overlapped dog seropositivity and historical flooding areas, demonstrating that domestic and asymptomatic dogs may be important environmental sentinels for leptospirosis in such urban areas, even in the absence of human seropositivity and low canine seropositivity. Associated risk factors for leptospirosis in dogs and humans according to multiple regression included healthy dogs (p = 0.02, odds ratio\u2014OR = 0.17, confidence interval of 95%CI 0.03\u20130.71) with indoor access (p = 0.059, OR = 5.12, 95%CI 1.10\u201337.7) and human males (p = 0.042, OR = 2.44, 95%CI 1.08\u20136.11) with presenting calf pain (p = 0.005, OR = 3.14, 95%CI 1.46\u20137. 14), jaundice (p &lt; 0.001, OR = 4.61, 95%CI 2.11\u201310.10) and renal failure (p = 0.008, OR = 4.49, 95%CI 1.49\u201313.76). The greater the rain precipitation (above 3 mm of average annual precipitation), the higher the number of leptospirosis cases in humans. In conclusion, dog active serosurvey and rain precipitation should be systematically reported and plotted altogether as a basis for the early detection and monitoring of human risk areas. Such findings may serve as a basis for public health policies in Brazil and other endemic countries worldwide.<\/jats:p>","DOI":"10.3390\/tropicalmed8090435","type":"journal-article","created":{"date-parts":[[2023,9,6]],"date-time":"2023-09-06T10:23:42Z","timestamp":1693995822000},"page":"435","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["One Health Approach to Leptospirosis: Dogs as Environmental Sentinels for Identification and Monitoring of Human Risk Areas in Southern Brazil"],"prefix":"10.3390","volume":"8","author":[{"given":"Natacha","family":"Sohn-Hausner","sequence":"first","affiliation":[{"name":"Department of Cell and Molecular Biology, Federal University of Paran\u00e1, Curitiba 80035-050, PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louise Bach","family":"Kmetiuk","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology, Federal University of Paran\u00e1, Curitiba 80035-050, PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evelyn Cristine","family":"da Silva","sequence":"additional","affiliation":[{"name":"Department of Veterinary Hygiene and Public Health, Sao Paulo State University, Botucatu 18618-681, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5127-0762","authenticated-orcid":false,"given":"Helio","family":"Langoni","sequence":"additional","affiliation":[{"name":"Department of Veterinary Hygiene and Public Health, Sao Paulo State University, Botucatu 18618-681, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4182-5821","authenticated-orcid":false,"given":"Alexander Welker","family":"Biondo","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology, Federal University of Paran\u00e1, Curitiba 80035-050, PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1007\/s10096-019-03797-4","article-title":"Leptospirosis: A Neglected Tropical Zoonotic Infection of Public Health Importance-an Updated Review","volume":"39","author":"Karpagam","year":"2020","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis. Off. Publ. Eur. Soc. Clin. Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1016\/S1473-3099(03)00830-2","article-title":"Leptospirosis: A Zoonotic Disease of Global Importance","volume":"3","author":"Bharti","year":"2003","journal-title":"Lancet. Infect. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1038\/nrmicro.2017.5","article-title":"Virulence of the Zoonotic Agent of Leptospirosis: Still Terra Incognita?","volume":"15","author":"Picardeau","year":"2017","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Sohn-Hausner, N., Kmetiuk, L.B., and Biondo, A.W. (2023). One Health Approach to Leptospirosis: Human-Dog Seroprevalence Associated to Socioeconomic and Environmental Risk Factors in Brazil over a 20-Year Period (2001\u20132020). Trop. Med. Infect. Dis., 8.","DOI":"10.3390\/tropicalmed8070356"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2036","DOI":"10.3389\/fmicb.2017.02036","article-title":"Benefits of Genomic Insights and CRISPR-Cas Signatures to Monitor Potential Pathogens across Drinking Water Production and Distribution Systems","volume":"8","author":"Zhang","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Bierque, E., Thibeaux, R., Girault, D., Soup\u00e9-Gilbert, M.-E., and Goarant, C. (2020). A Systematic Review of Leptospira in Water and Soil Environments. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0227055"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1007\/s00284-015-0836-4","article-title":"Waterborne Leptospirosis: Survival and Preservation of the Virulence of Pathogenic Leptospira spp. in Fresh Water","volume":"71","author":"Aviat","year":"2015","journal-title":"Curr. Microbiol."},{"key":"ref_8","first-page":"219","article-title":"Some Effects of Various Environmental Conditions on the Survival of Leptospira pomona","volume":"18","author":"Okazaki","year":"1957","journal-title":"Am. J. Vet. Res."},{"key":"ref_9","first-page":"130","article-title":"Genetic Evidence for a Potential Environmental Pathway to Spillover Infection of Rat-Borne Leptospirosis","volume":"225","author":"Schneider","year":"2021","journal-title":"J. Infect. Dis."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"580400","DOI":"10.3389\/fvets.2020.580400","article-title":"Spatial and Simultaneous Seroprevalence of Anti-Leptospira Antibodies in Owners and Their Domiciled Dogs in a Major City of Southern Brazil","volume":"7","author":"Benitez","year":"2021","journal-title":"Front. Vet. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1590\/0037-8682-0315-2016","article-title":"Serosurvey of Anti-Leptospira sp. and Anti-Toxoplasma gondii Antibodies in Capybaras and Collared and White-Lipped Peccaries","volume":"50","author":"Ullmann","year":"2017","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Costa, F., Hagan, J.E., Calcagno, J., Kane, M., Torgerson, P., Martinez-Silveira, M.S., Stein, C., Abela-Ridder, B., and Ko, A.I. (2015). Global Morbidity and Mortality of Leptospirosis: A Systematic Review. PLoS Negl. Trop. Dis., 9.","DOI":"10.1371\/journal.pntd.0003898"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Torgerson, P.R., Hagan, J.E., Costa, F., Calcagno, J., Kane, M., Martinez-Silveira, M.S., Goris, M.G.A., Stein, C., Ko, A.I., and Abela-Ridder, B. (2015). Global Burden of Leptospirosis: Estimated in Terms of Disability Adjusted Life Years. PLoS Negl. Trop. Dis., 9.","DOI":"10.1371\/journal.pntd.0004122"},{"key":"ref_14","unstructured":"(2023, July 05). Sa\u00fade., B.M.d.S.S.d.V.e.S.D.d.A.E.d.V. em Guia de Vigil\u00e2ncia Em Sa\u00fade 2022, 1, 126. Brazilian Ministry of Health, Available online: https:\/\/bvsms.saude.gov.br\/bvs\/publicacoes\/guia_vigilancia_saude_5ed_rev_atual.pdf."},{"key":"ref_15","first-page":"1","article-title":"Leptospirose: Situa\u00e7\u00e3o Epidemiol\u00f3gica Do Brasil No Per\u00edodo de 2007 a 2016","volume":"49","author":"Brasil","year":"2018","journal-title":"Bol. Epidemiol."},{"key":"ref_16","unstructured":"Sinan, M.d.S. (2023, July 08). Sistema de Informa\u00e7\u00e3o de Agravos de Notifica\u00e7\u00e3o, Available online: https:\/\/portalsinan.saude.gov.br\/."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1590\/0103-1104202012616","article-title":"An\u00e1lise Espacial Da Leptospirose No Brasil","volume":"44","author":"Marteli","year":"2020","journal-title":"Sa\u00fade Debate"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1590\/S1984-29612012000200006","article-title":"Dog Parasite Incidence and Risk Factors, from Sampling after One-Year Interval, in Pinhais, Brazil","volume":"21","author":"Martins","year":"2012","journal-title":"Rev. Bras. Parasitol. Veter."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1590\/0037-8682-0213-2014","article-title":"Seroprevalence and Seroincidence of Leptospira Infection in Dogs during a One-Year Period in an Endemic Urban Area in Southern Brazil","volume":"48","author":"Morikawa","year":"2015","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1111\/jsap.12328","article-title":"European Consensus Statement on Leptospirosis in Dogs and Cats","volume":"56","author":"Schuller","year":"2015","journal-title":"J. Small Anim. Pract."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"59","DOI":"10.5123\/S2176-62232015000300008","article-title":"Leptospirose Canina e Humana, Uma Poss\u00edvel Transmiss\u00e3o Conjuntival No Munic\u00edpio de S\u00e3o Paulo, Estado de S\u00e3o Paulo, Brasil","volume":"6","author":"Polachini","year":"2015","journal-title":"Rev. Pan-Amaz. Sa\u00fade"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1590\/S0103-84781998000100018","article-title":"Aglutininas Anti-Leptosp\u00edricas Em C\u00e3es Na \u00c1rea de Influ\u00eancia Do Centro de Controle de Zoonoses, Pelotas, RS, Brasil, No Ano de 1995","volume":"28","author":"Avila","year":"1998","journal-title":"Ci\u00eanc. Rural"},{"key":"ref_23","first-page":"294","article-title":"Evidence of dog as a reservoir for human leptospirosis: A serovar isolation, molecular characterization and its use in a serological survey","volume":"38","author":"Dellagostin","year":"2005","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1998","DOI":"10.1590\/S0103-84782013001100013","article-title":"Domestic Dogs in Rural Area of Fragmented Atlantic Forest: Potential Threats to Wild Animals","volume":"43","author":"Martinez","year":"2013","journal-title":"Ci\u00eanc. Rural"},{"key":"ref_25","unstructured":"WHO (2023, July 08). Integrated Leptospirosis Surveillance through a Single Health Approach. Available online: https:\/\/www.who.int\/indonesia\/news\/detail\/18-05-2022-integrated-leptospirosis-surveillance-through-a-one-health-approach."},{"key":"ref_26","unstructured":"Coordena\u00e7\u00e3o da Regi\u00e3o Metropolitana de Curitiba COMEC, C.D.A.R.M.D.E.C (2023, July 08). Munic\u00edpios Da Regi\u00e3o Metropolitana de Curitiba, Available online: https:\/\/www.comec.pr.gov.br\/FAQ\/Municipios-da-Regiao-Metropolitana-de-Curitiba."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1127\/0941-2948\/2013\/0507","article-title":"Koppen\u2019s Climate Classification Map for Brazil","volume":"22","author":"Alvares","year":"2013","journal-title":"Meteorol. Zeitschrif"},{"key":"ref_28","unstructured":"(2023, July 08). Brazilian Institute of Geography and Statistics IBGE, I.B. de G. e E. Cidades, Available online: https:\/\/cidades.ibge.gov.br\/brasil\/panorama."},{"key":"ref_29","unstructured":"PINHAIS, P.M.D.E.P. (2023, July 08). Plano de Habita\u00e7\u00e3o e Regulariza\u00e7\u00e3o Fundi\u00e1ria: Diagn\u00f3stico Final. Available online: https:\/\/silo.tips\/queue\/prefeito-municipal-luis-goularte-alves?&queue_id=-1&v=1659892266&u=MjgwNDoxNGM6ODdjMzpjNjY2OjQ5NjQ6M2VjNDo5MzcwOjYyZDU=."},{"key":"ref_30","unstructured":"(2022, January 05). R: The R Project for Statistical Computing. Available online: https:\/\/www.r-project.org\/."},{"key":"ref_31","unstructured":"(2023, July 08). Prefeitura Municipal de Pinhais, PINHAIS, P.M.D.E.P. GeoPinhais: Mapa Pinhais, Available online: http:\/\/geo.pinhais.pr.gov.br\/geo\/mapa\/pinhais\/."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.tmaid.2017.07.010","article-title":"Human and Animal Leptospirosis in Southern Brazil: A Five-Year Retrospective Study","volume":"18","author":"Jorge","year":"2017","journal-title":"Travel Med. Infect. Dis."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1590\/s1984-29612019043","article-title":"Epidemiological Relevance of Dogs for the Prevention of Toxoplasma gondii, Neospora caninum and Leptospira spp.","volume":"28","author":"Pasquali","year":"2019","journal-title":"Rev. Bras. Parasitol. Veter."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"e09484","DOI":"10.1016\/j.heliyon.2022.e09484","article-title":"Serological and Molecular Epidemiology of Leptospirosis and the Role of Dogs as Sentinel for Human Infection in Nigeria","volume":"8","author":"Pilau","year":"2022","journal-title":"Heliyon"},{"key":"ref_35","unstructured":"(2023, July 11). Sistema de Informa\u00e7\u00e3o de Agravos de Notifica\u00e7\u00e3o. Sinan, M.d.S. Leptospirose: Instrumentos Para Registro e An\u00e1lise, Available online: http:\/\/portalsinan.saude.gov.br\/leptospirose."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Smith, A.M., Arruda, A.G., Evason, M.D., Weese, J.S., Wittum, T.E., Szlosek, D., and Stull, J.W. (2019). A Cross-Sectional Study of Environmental, Dog, and Human-Related Risk Factors for Positive Canine Leptospirosis PCR Test Results in the United States, 2009 to 2016. BMC Vet. Res., 15.","DOI":"10.1186\/s12917-019-2148-6"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Guerra-Silveira, F., and Abad-Franch, F. (2013). Sex Bias in Infectious Disease Epidemiology: Patterns and Processes. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0062390"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1590\/S0100-736X2013000100013","article-title":"Spatial Analysis of the Risk of Canine Leptospirosis in the Vila Pantanal, Curitiba, Paran\u00e1, Brazil","volume":"33","author":"Bier","year":"2013","journal-title":"Pesqui. Vet. Bras."},{"key":"ref_39","first-page":"83","article-title":"Investigation about Knowledge of People Infected by Leptospira spp. and Seroprevalence, Leptospiruria and Leptospiraemia of Their Pet Dogs","volume":"24","author":"Cordeiro","year":"2019","journal-title":"Arch. Vet. Sci."},{"key":"ref_40","first-page":"30683","article-title":"Serological Survey of Leptospira spp. and Toxoplasma gondii in Companion Animals Rescued From Hoarding Behavior Cases in Curitiba, Paran\u00e1, Brazil","volume":"9","author":"Pellizzaro","year":"2019","journal-title":"Int. J. Dev. Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1002\/vms3.704","article-title":"Serological Survey of Anti-Leptospira spp. Antibodies in Individuals with Animal Hoarding Disorder and Their Dogs in a Major City of Southern Brazil","volume":"8","author":"Pellizzaro","year":"2022","journal-title":"Vet. Med. Sci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"253","DOI":"10.5433\/1679-0359.2015v36n1p253","article-title":"Zoonoses Em C\u00e3es e Gatos Atendidos Em Projeto de Controle de Natalidade: Toxoplasma gondii, Leishmania spp. e Leptospira spp., Sorodiagn\u00f3stico e Epidemiologia","volume":"36","author":"Caldart","year":"2015","journal-title":"Semin. Ci\u00eanc. Agr\u00e1r."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"167","DOI":"10.5433\/1679-0359.2018v39n1p167","article-title":"Detection of Anti-Leptospira spp., Anti-Brucella spp., and Anti-Toxoplasma gondii Antibodies in Stray Dogs","volume":"39","author":"Hafemann","year":"2018","journal-title":"Semin. Ci\u00eanc. Agr\u00e1r."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1590\/S0037-86822012000100019","article-title":"Geographic Analysis on the Occurrence of Human and Canine Leptospirosis in the City of Maring\u00e1, State of Paran\u00e1, Brazil","volume":"45","author":"Fonzar","year":"2012","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"3201","DOI":"10.5433\/1679-0359.2012v33Supl2p3201","article-title":"de Soroepidemiologia Da Leptospirose Em C\u00e3es Domiciliados Na \u00c1rea Urbana Do Munic\u00edpio de Jataizinho, Paran\u00e1","volume":"33","author":"Benitez","year":"2013","journal-title":"Semin. Ci\u00eanc. Agr\u00e1r."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1590\/0037-8682-1665-2013","article-title":"Incidence of Canine Leptospirosis in the Metropolitan Area of Curitiba, State of Paran\u00e1, Southern Brazil","volume":"46","author":"Martins","year":"2013","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1186\/1678-9199-19-23","article-title":"Toxoplasmosis, Leptospirosis and Brucellosis in Stray Dogs Housed at the Shelter in Umuarama Municipality, Parana, Brazil","volume":"19","author":"Goncalves","year":"2013","journal-title":"J. Venom. Anim. Toxins Incl. Trop. Dis."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1111\/zph.12236","article-title":"Meta-Analyses of Factors Associated with Leptospirosis in Domestic Dogs","volume":"63","author":"Monti","year":"2016","journal-title":"Zoonoses Public Health"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"105037","DOI":"10.1016\/j.prevetmed.2020.105037","article-title":"Meta-Analysis of Risk Factors for Canine Leptospirosis","volume":"181","author":"Ricardo","year":"2020","journal-title":"Prev. Vet. Med."},{"key":"ref_50","unstructured":"(2023, August 26). SISDC Sistema de Defesa Civil, Available online: http:\/\/www.sisdc.pr.gov.br\/sdc\/publico\/relatorios\/ocorrencias_geral.jsp."},{"key":"ref_51","unstructured":"(2023, August 26). Prefeitura Municipal de Pinhais, PINHAIS, P.M.D.E.P. DECRETO N\u00b0 914 2010. Available online: https:\/\/leismunicipais.com.br\/a\/pr\/p\/pinhais\/decreto\/2010\/92\/914\/decreto-n-914-2010-declara-em-situacao-anormal-caracterizada-como-situacao-de-emergencia-as-areas-do-municipio-afetadas-por-inundacoes-vendavais-e-tempestades-2010-04-23-versao-original."},{"key":"ref_52","unstructured":"(2023, August 26). Prefeitura Municipal de Pinhais, PINHAIS, P.M.D.E.P. DECRETO N\u00b0 926 2010. Available online: https:\/\/leismunicipais.com.br\/a1\/pr\/p\/pinhais\/decreto\/2010\/92\/926\/decreto-n-926-2010-declara-em-situacao-anormal-caracterizada-como-situacao-a-area-do-municipio-afetada-de-emergencia-por-ne-hal-12303-alagamentos?r=p."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"286","DOI":"10.15361\/2175-0106.2020v36n4p286-300","article-title":"Leptospirose: An\u00e1lise Bibliogr\u00e1fica, Inqu\u00e9rito Sorol\u00f3gico E Vacina\u00e7\u00e3o Canina","volume":"36","author":"Silvestrini","year":"2020","journal-title":"Ars Vet."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1678","DOI":"10.1590\/0001-3765202220201026","article-title":"A Systematic Review of the Geographic Distribution of Pathogenic Leptospira Serovars in the Americas, 1930\u20132017","volume":"94","author":"Browne","year":"2022","journal-title":"An. Acad. Bras. Ci\u00eanc."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.actatropica.2008.07.005","article-title":"Carriage of Leptospira interrogans among Domestic Rats from an Urban Setting Highly Endemic for Leptospirosis in Brazil","volume":"108","author":"Calderwood","year":"2008","journal-title":"Acta Trop."},{"key":"ref_56","first-page":"1","article-title":"Research for Toxoplasma gondii, Leptospira spp. And Leishmania spp. antibodies in dogs from Pardinho, S\u00e3o Paulo","volume":"28","author":"Mantovan","year":"2021","journal-title":"Vet. Zootec."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"e02373","DOI":"10.1016\/j.heliyon.2019.e02373","article-title":"Leptospira Seroprevalence in Owned Dogs from Spain","volume":"5","author":"Vila","year":"2019","journal-title":"Heliyon"},{"key":"ref_58","first-page":"89","article-title":"Leptospirosis at Human-Animal-Environment Interfaces in Latin-America: Drivers, Prevention, and Control Measures","volume":"23","year":"2021","journal-title":"Biotecnia"},{"key":"ref_59","first-page":"656","article-title":"Inqu\u00e9rito Sorol\u00f3gico e Distribui\u00e7\u00e3o Espacial Da Leptospirose Canina Em \u00c1rea Territorial Urbana Da Cidade de Botucatu","volume":"16","author":"Silva","year":"2009","journal-title":"Vet. Zootec."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1111\/j.1748-5827.1994.tb03275.x","article-title":"Serological Findings in Cases of Acute Leptospirosis in the Dog","volume":"35","author":"Scanziani","year":"1994","journal-title":"J. Small Anim. Pract."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1952","DOI":"10.1590\/S0102-311X2005000600046","article-title":"Occurrence of Leptospira spp. soropositive stray dogs in Itapema, Santa Catarina, Brazil","volume":"21","author":"Blazius","year":"2005","journal-title":"Cad. Saude Publica"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1590\/1808-1657v67n2p1812000","article-title":"Leptospirose Em C\u00e3es: Preval\u00eancia E Fatores De Riscono Meio Rural Do Munic\u00edpio De Pelotas, RS","volume":"67","author":"Jouglard","year":"2000","journal-title":"Arq. Inst. Biol"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2017\/5904757","article-title":"Leptospira Seroprevalence in Domestic Dogs and Cats on the Caribbean Island of Saint Kitts","volume":"2017","author":"Pratt","year":"2017","journal-title":"Vet. Med. Int."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Koizumi, N., Izumiya, H., and Ohnishi, M. (2020). Genetic Relatedness of Leptospira interrogans Serogroup Autumnalis Isolated from Humans, Dogs, and Mice in Japan. BMC Res. Notes, 13.","DOI":"10.1186\/s13104-020-05211-1"},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Santos, C.M., Dias, G.C.D.R.S., Saldanha, A.V.P., Esteves, S.B., Cortez, A., Guedes, I.B., Heinemann, M.B., Gon\u00e7ales, A.P., and Miotto, B.A. (2021). Molecular and Serological Characterization of Pathogenic Leptospira spp. Isolated from Symptomatic Dogs in a Highly Endemic Area, Brazil. BMC Vet. Res., 17.","DOI":"10.1186\/s12917-021-02930-w"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1852","DOI":"10.1017\/S0950268817000632","article-title":"High Number of Asymptomatic Dogs as Leptospiral Carriers in an Endemic Area Indicates a Serious Public Health Concern","volume":"145","author":"Vieira","year":"2017","journal-title":"Epidemiol. Infect."},{"key":"ref_67","first-page":"919","article-title":"Leptospirose Canina\u2014Revis\u00e3o de Literatura","volume":"4","author":"Salaberry","year":"2010","journal-title":"Pubvet"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1111\/zph.12557","article-title":"Despite High-Risk Exposures, No Evidence of Zoonotic Transmission during a Canine Outbreak of Leptospirosis","volume":"66","author":"Guagliardo","year":"2019","journal-title":"Zoonoses Public Health"},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Mwachui, M.A., Crump, L., Hartskeerl, R., Zinsstag, J., and Hattendorf, J. (2015). Environmental and Behavioural Determinants of Leptospirosis Transmission: A Systematic Review. PLoS Negl. Trop. Dis., 9.","DOI":"10.1371\/journal.pntd.0003843"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"e006946","DOI":"10.1136\/bmjgh-2021-006946","article-title":"Estimating the Burden of Leptospirosis: Global Lessons from Sri Lanka","volume":"6","author":"Warnasekara","year":"2021","journal-title":"BMJ Glob. Health"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1590\/1413-81232020253.16442018","article-title":"A Leptospirose Humana Como Doen\u00e7a Duplamente Negligenciada No Brasil","volume":"25","author":"Martins","year":"2020","journal-title":"Ci\u00eanc. Saude Colet."},{"key":"ref_72","first-page":"1","article-title":"The Vicious Circle of the Leptospirosis Negligence in Brazil","volume":"76","author":"Rodrigues","year":"2017","journal-title":"Rev. Inst. Adolfo. Lutz."},{"key":"ref_73","unstructured":"World Health Organization (2023, July 08). Human Leptospirosis: Guidance for Diagnosis, Surveillance and Control, Available online: https:\/\/apps.who.int\/iris\/handle\/10665\/42667."},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Victoriano, A.F.B., Smythe, L.D., Gloriani-Barzaga, N., Cavinta, L.L., Kasai, T., Limpakarnjanarat, K., Ong, B.L., Gongal, G., Hall, J., and Coulombe, C.A. (2009). Leptospirosis in the Asia Pacific Region. BMC Infect. Dis., 9.","DOI":"10.1186\/1471-2334-9-147"}],"container-title":["Tropical Medicine and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2414-6366\/8\/9\/435\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:45:46Z","timestamp":1760129146000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2414-6366\/8\/9\/435"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,6]]},"references-count":74,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2023,9]]}},"alternative-id":["tropicalmed8090435"],"URL":"https:\/\/doi.org\/10.3390\/tropicalmed8090435","relation":{},"ISSN":["2414-6366"],"issn-type":[{"value":"2414-6366","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9,6]]}}}