{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T20:34:56Z","timestamp":1791491696905,"version":"4.3.4"},"reference-count":38,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T00:00:00Z","timestamp":1696896000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior (CAPES)","award":["28\/2018"],"award-info":[{"award-number":["28\/2018"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior (CAPES)","award":["5.22\/2022"],"award-info":[{"award-number":["5.22\/2022"]}]},{"name":"Funda\u00e7\u00e3o Oswaldo Cruz","award":["28\/2018"],"award-info":[{"award-number":["28\/2018"]}]},{"name":"Funda\u00e7\u00e3o Oswaldo Cruz","award":["5.22\/2022"],"award-info":[{"award-number":["5.22\/2022"]}]},{"name":"FAPEMIG","award":["28\/2018"],"award-info":[{"award-number":["28\/2018"]}]},{"name":"FAPEMIG","award":["5.22\/2022"],"award-info":[{"award-number":["5.22\/2022"]}]},{"name":"Conselho Nacional Cient\u0131fico e Tecnologico (CNPq)","award":["28\/2018"],"award-info":[{"award-number":["28\/2018"]}]},{"name":"Conselho Nacional Cient\u0131fico e Tecnologico (CNPq)","award":["5.22\/2022"],"award-info":[{"award-number":["5.22\/2022"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>Canine visceral leishmaniasis (CVL) remains a significant disease worldwide. In Brazil, its treatment is performed using miltefosine, which has demonstrated promising outcomes in dogs. This study represents the first attempt to treat and monitor dogs with CVL in natural conditions over the course of one year. The dogs were divided into two groups: G1 received miltefosine and allopurinol for 28 days, while G2 received miltefosine for 28 days, followed by allopurinol for one year. The follow-up involved clinical, hematological, and biochemical evaluations, as well as the detection of Leishmania DNA in skin and bone marrow samples. By the end of the follow-up, dogs in G2 exhibited improved staging compared to their initial conditions, whereas those in G1 showed worsened staging. Leishmania DNA in skin and bone marrow decreased between 6 and 12 months after treatment. Our observations indicate that the treatment using miltefosine reduces the detection of the parasite in the skin and bone marrow for up to one year following its administration. The continuous use of allopurinol contributes to control of the disease in dogs. These findings provide valuable insights into the response of dogs treated in natural conditions, offering essential information for veterinarians and public health authorities.<\/jats:p>","DOI":"10.3390\/tropicalmed8100472","type":"journal-article","created":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T10:23:42Z","timestamp":1696933422000},"page":"472","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Clinical and Laboratory Response of Domiciled Dogs with Visceral Leishmaniasis Treated with Miltefosine and Allopurinol"],"prefix":"10.3390","volume":"8","author":[{"given":"Talita Pereira","family":"Vaz","sequence":"first","affiliation":[{"name":"Ren\u00e9 Rachou Institute (FIOCRUZ\/MG), Av. Augusto de Lima, 1715, Barro Preto, Belo Horizonte 30190-009, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8274-4166","authenticated-orcid":false,"given":"Patr\u00edcia Fl\u00e1via","family":"Quaresma","sequence":"additional","affiliation":[{"name":"Campus Reitor Jo\u00e3o Davi Ferreira Lima, Federal University of Santa Catarina, Trindade, Florian\u00f3polis 88040-900, SC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2799-8267","authenticated-orcid":false,"given":"Felipe Dutra","family":"R\u00eago","sequence":"additional","affiliation":[{"name":"Ren\u00e9 Rachou Institute (FIOCRUZ\/MG), Av. Augusto de Lima, 1715, Barro Preto, Belo Horizonte 30190-009, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camila Binder","family":"Souza","sequence":"additional","affiliation":[{"name":"Ren\u00e9 Rachou Institute (FIOCRUZ\/MG), Av. Augusto de Lima, 1715, Barro Preto, Belo Horizonte 30190-009, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3930-547X","authenticated-orcid":false,"given":"Gilberto","family":"Fontes","sequence":"additional","affiliation":[{"name":"Campus Centro Oeste, Federal University of S\u00e3o Jo\u00e3o del Rei (UFSJ), Sebasti\u00e3o Gon\u00e7alves Coelho, 400, Divin\u00f3polis 35501-296, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7034-535X","authenticated-orcid":false,"given":"C\u00e9lia Maria Ferreira","family":"Gontijo","sequence":"additional","affiliation":[{"name":"Ren\u00e9 Rachou Institute (FIOCRUZ\/MG), Av. Augusto de Lima, 1715, Barro Preto, Belo Horizonte 30190-009, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,10]]},"reference":[{"key":"ref_1","unstructured":"WHO (2023, June 09). 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