{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T11:07:23Z","timestamp":1787828843214,"version":"build-2784847793"},"reference-count":41,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2024,4,6]],"date-time":"2024-04-06T00:00:00Z","timestamp":1712361600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>Background: Although the Coxiella burnetii infection has been investigated in dogs, its role in human transmission remains to be fully established, particularly in close and daily human\u2013dog contact settings, such as in Police K-9 Units. Methods: Accordingly, this study aimed to assess anti-C. burnetii antibodies in clinically healthy police officers by an in-house indirect immunofluorescence assay (IFA), and working dogs by a commercial IFA Kit, from the State Special Operations Battalion, Paran\u00e1, Southern Brazil. Results: Overall, 1\/18 (5.5%) police officers and 9\/30 (30.0%; CI 95% 16.66\u201347.88) dogs tested seropositive to anti-C. burnetii IgG antibodies. Conclusions: To date, this is the highest prevalence of Q fever seropositivity among military dogs worldwide. Despite the low sampling rate, a statistically significant association was found between seropositivity and female dogs (p = 0.0492). Further studies with larger sample sizes should be conducted to establish the prevalence of Q Fever in other Brazilian K-9 Units. In summary, this study is the first to conduct a concomitant serosurvey of police officers and working dogs, and its findings should be considered a warning for cross-exposure and transmission of Coxiella burnetii among Police K-9 Units in Brazil and worldwide.<\/jats:p>","DOI":"10.3390\/tropicalmed9040078","type":"journal-article","created":{"date-parts":[[2024,4,8]],"date-time":"2024-04-08T12:01:22Z","timestamp":1712577682000},"page":"78","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Serosurvey of Coxiella burnetii in Police Officers and Working Dogs in Brazil: Case Report and One Health Implications"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1178-5643","authenticated-orcid":false,"given":"Danilo Alves","family":"de Fran\u00e7a","sequence":"first","affiliation":[{"name":"Department of Animal Production and Preventive Veterinary Medicine, School of Veterinary Medicine and Animals Science, S\u00e3o Paulo State University, Botucatu 05508-220, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9ssica Santos","family":"da Silva","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Federal University of Paran\u00e1 State, Curitiba 81530-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N\u00e1ssarah Jabur Lot","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Department of Animal Production and Preventive Veterinary Medicine, School of Veterinary Medicine and Animals Science, S\u00e3o Paulo State University, Botucatu 05508-220, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana \u00cdris de Lima","family":"Dur\u00e9","sequence":"additional","affiliation":[{"name":"Service of Virology and Rickettsiosis, Octavio Magalhaes Institute, Ezequiel Dias Foundation, Belo Horizonte 30510-010, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Henrique","family":"Farinhas","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Federal University of Paran\u00e1 State, Curitiba 81530-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6579-1278","authenticated-orcid":false,"given":"Louise Bach","family":"Kmetiuk","sequence":"additional","affiliation":[{"name":"Zoonoses Surveillance Unit, Municipal Secretary of Health, Curitiba 81265-320, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5127-0762","authenticated-orcid":false,"given":"Helio","family":"Langoni","sequence":"additional","affiliation":[{"name":"Department of Animal Production and Preventive Veterinary Medicine, School of Veterinary Medicine and Animals Science, S\u00e3o Paulo State University, Botucatu 05508-220, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4182-5821","authenticated-orcid":false,"given":"Alexander Welker","family":"Biondo","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Federal University of Paran\u00e1 State, Curitiba 81530-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e39","DOI":"10.1590\/s1678-9946202365039","article-title":"Overview of Q Fever in Brazil: An Underestimated Zoonosis","volume":"65","author":"Fernandes","year":"2023","journal-title":"Rev. Inst. Med. Trop. Sao Paulo"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"de Fran\u00e7a, D.A., de Souza Ribeiro Mioni, M., Fornazari, F., de Lima Dur\u00e9, A.\u00cd., Silva, M.V.F., Possebon, F.S., Richini-Pereira, V.B., Langoni, H., and Megid, J. (2022). Seropositivity for Coxiella burnetii in Suspected Patients with Dengue in S\u00e3o Paulo State, Brazil. PLoS Negl. Trop. Dis., 16.","DOI":"10.1371\/journal.pntd.0010392"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"100660","DOI":"10.1016\/j.onehlt.2023.100660","article-title":"Coxiella burnetii Seroprevalence in Sheep Herd from Paraguay: First Evidence of Bacterial Circulation in the Country","volume":"18","author":"Iglesias","year":"2024","journal-title":"One Health"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.vetmic.2009.07.016","article-title":"Q Fever","volume":"140","author":"Angelakis","year":"2010","journal-title":"Vet. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0378-1135(98)00247-8","article-title":"Survey of Seroprevalence of Q Fever in Dogs in the Southeast of France, French Guyana, Martinique, Senegal and the Ivory Coast","volume":"64","author":"Boni","year":"1998","journal-title":"Vet. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1111\/zph.12250","article-title":"Seroprevalence of Coxiella burnetii in Australian Dogs","volume":"63","author":"Shapiro","year":"2016","journal-title":"Zoonoses Public Health"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1111\/avj.13151","article-title":"Seroprevalence of Coxiella burnetii in Pig-Hunting Dogs from North Queensland, Australia","volume":"100","author":"Orr","year":"2022","journal-title":"Aust. Vet. J."},{"key":"ref_8","first-page":"117","article-title":"The Isolation of Coxiella burnetii from Rhipicephalus Sanguineus on Naturally Infected Dogs","volume":"122","author":"Mantovani","year":"1953","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_9","first-page":"98","article-title":"Estudo Do Perfil Dermatogl\u00edfico, Somatot\u00edpico e Das Qualidades F\u00edsicas Dps Policiais Do Batalh\u00e3o de Opera\u00e7\u00f5es Especiais (PMERJ) Do Ano de 2005","volume":"6","year":"2007","journal-title":"Fit. Perform. J."},{"key":"ref_10","first-page":"67","article-title":"Sero-Epidemiological Studies on Coxiellosis in Animals and Man in the State of Uttar Pradesh and Delhi (India)","volume":"6","author":"Yadav","year":"1979","journal-title":"Int. J. Zoonoses"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1093\/oxfordjournals.aje.a112919","article-title":"Environmental Exposure to Coxiella burnetii: A Sero-Epidemiologic Survey among Domestic Animals","volume":"111","author":"Willeberg","year":"1980","journal-title":"Am. J. Epidemiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"763","DOI":"10.2105\/AJPH.75.7.763","article-title":"Seroepidemiology of Q Fever among Domestic Animals in Nova Scotia","volume":"75","author":"Marrie","year":"1985","journal-title":"Am. J. Public Health"},{"key":"ref_13","first-page":"30","article-title":"Infections with Coxiella burnetii in Man and Animals in The Netherlands","volume":"267","author":"Houwers","year":"1987","journal-title":"Zentralblatt Bakteriol. Mikrobiol. Hyg. Ser. A Med. Microbiol. Infect. Dis. Virol. Parasitol."},{"key":"ref_14","first-page":"493","article-title":"Dog-Transmitted Zoonoses: A Serological Survey in the Province of Bologna","volume":"28","author":"Baldelli","year":"1992","journal-title":"Ann. Ist. Super. Sanita"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1136\/vr.131.21.490","article-title":"Seroepidemiology of Coxiella burnetii in Domestic and Companion Animals in Japan","volume":"131","author":"Htwe","year":"1992","journal-title":"Vet. Rec."},{"key":"ref_16","first-page":"100","article-title":"Antibodies against Rickettsiaceae in Dogs of Set\u00fabal, Portugal","volume":"3","author":"Bacellar","year":"1995","journal-title":"Cent. Eur. J. Public Health"},{"key":"ref_17","first-page":"339","article-title":"Antibodies to Spotted Fever Group Rickettsiae and Coxiella burnetii among Domestic Animals in Southern Croatia","volume":"42","author":"Poljak","year":"1995","journal-title":"Acta Microbiol. Immunol. Hung."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.7205\/MILMED-D-11-00025","article-title":"A Comparison of the Serological Evidence of Coxiella burnetii Exposure between Military Working Dogs and Feral Canines in Iraq","volume":"176","author":"Havas","year":"2011","journal-title":"Mil. Med."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1111\/j.1751-0813.2011.00819.x","article-title":"Serological Evidence of Coxiella burnetii Infection in Dogs in a Regional Centre","volume":"89","author":"Cooper","year":"2011","journal-title":"Aust. Vet. J."},{"key":"ref_20","first-page":"87","article-title":"Serological and Molecular Evidence of Coxiella burnetii in Samples from Humans and Animals in China","volume":"23","author":"Kelly","year":"2016","journal-title":"Ann. Agric. Environ. Med."},{"key":"ref_21","first-page":"34","article-title":"Seroprevalence of Lyme Disease and Q Fever in Referred Dogs to Veterinary Hospital of Ahvaz","volume":"11","author":"Rezaei","year":"2016","journal-title":"Iran. Vet. J."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1089\/vbz.2016.1977","article-title":"Prevalence of Antibodies Against Coxiella burnetii in Korean Native Cattle, Dairy Cattle, and Dogs in South Korea","volume":"17","author":"Lyoo","year":"2017","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"769","DOI":"10.2478\/s11686-019-00098-w","article-title":"Seroprevalences of Rickettsia Conorii, Ehrlichia Canis and Coxiella burnetii in Dogs from Montenegro","volume":"64","year":"2019","journal-title":"Acta Parasitol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Ebani, V.V. (2020). Retrospective Study on the Occurrence of Antibodies against Coxiella burnetii in Dogs from Central Italy. Pathogens, 9.","DOI":"10.3390\/pathogens9121068"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Saengsawang, P., Pangjai, D., Kaewmongkol, G., and Inpankaew, T. (2022). Detection of Antibodies against Three Zoonotic bartonella spp. and Cross-Reactivity among Species and Coxiella burnetii in Dogs and Cats from Central Thailand. Comp. Immunol. Microbiol. Infect. Dis., 81.","DOI":"10.1016\/j.cimid.2021.101743"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Di Cataldo, S., Cevidanes, A., Ulloa-Contreras, C., Hidalgo-Hermoso, E., Gargano, V., Cabello, J., Sacrist\u00e1n, I., Napolitano, C., Gambino, D., and Vicari, D. (2022). A Serosurvey for Spotted Fever Group Rickettsia and Coxiella burnetii Antibodies in Rural Dogs and Foxes, Chile. Comp. Immunol. Microbiol. Infect. Dis., 83.","DOI":"10.1016\/j.cimid.2022.101769"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"de Fran\u00e7a, D.A., Kmetiuk, L.B., Panazzolo, G.A., Domingues, O.J., da Silva, F.P., Biondo, L.M., de Souza Ribeiro Mioni, M., Possebon, F.S., de Lima Dur\u00e9, A.\u00cd., and Silva, M.V. (2024). Serosurvey of Coxiella burnetii in Descendants of Former Black Slaves (Quilombola Communities) of Southern Brazil. Microorganisms, 12.","DOI":"10.3390\/microorganisms12010092"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"de Fran\u00e7a, D.A., de Souza Ribeiro Mioni, M., Fornazari, F., Rodrigues, N.J.L., Polido, L.R.F., Appolinario, C.M., Ribeiro, B.L.D., de Lima Dur\u00e9, A.\u00cd., Silva, M.V.F., and Richini-Pereira, V.B. (2023). Comparison of Three Serologic Tests for the Detection of Anti-Coxiella burnetii Antibodies in Patients with Q Fever. Pathogens, 12.","DOI":"10.3390\/pathogens12070873"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1186\/s13071-018-2629-7","article-title":"First Molecular Detection and Genetic Characterization of Coxiella burnetii in Zambian Dogs and Rodents","volume":"11","author":"Chitanga","year":"2018","journal-title":"Parasit. Vectors"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.4102\/jsava.v88i0.1390","article-title":"Occurrence of Coxiella burnetii, Ehrlichia canis, Rickettsia Species and Anaplasma phagocytophilum-like Bacterium in Ticks Collected from Dogs and Cats in South Africa","volume":"88","author":"Mtshali","year":"2017","journal-title":"J. S. Afr. Vet. Assoc."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1186\/s13071-023-06010-w","article-title":"One Health Approach to Toxocariasis in Quilombola Communities of Southern Brazil","volume":"16","author":"Panazzolo","year":"2023","journal-title":"Parasit. Vectors"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1111\/zph.12707","article-title":"Molecular Detection of Coxiella burnetii in Raw Meat Intended for Pet Consumption","volume":"67","author":"Shapiro","year":"2020","journal-title":"Zoonoses Public Health"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1007\/s40475-023-00288-7","article-title":"Coxiella burnetii Infection in Livestock, Pets, Wildlife, and Ticks in Latin America and the Caribbean: A Comprehensive Review of the Literature","volume":"10","author":"Epelboin","year":"2023","journal-title":"Curr. Trop. Med. Reports"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1016\/j.pt.2015.06.014","article-title":"The Importance of Ticks in Q Fever Transmission: What Has (and Has Not) Been Demonstrated?","volume":"31","author":"Duron","year":"2015","journal-title":"Trends Parasitol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"e009822","DOI":"10.1590\/s1984-29612022051","article-title":"Q Fever and Coxiellosis in Brazil: An Underestimated Disease? A Brief Review","volume":"31","author":"Labruna","year":"2022","journal-title":"Rev. Bras. Parasitol. Vet."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"796","DOI":"10.2460\/javma.2002.221.796","article-title":"Q Fever","volume":"221","author":"McQuiston","year":"2002","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1093\/clinids\/23.4.753","article-title":"A Dog-Related Outbreak of Q Fever","volume":"23","author":"Buhariwalla","year":"1996","journal-title":"Clin. Infect. Dis."},{"key":"ref_38","first-page":"1","article-title":"Diagnosis and Management of Q Fever\u2014United States, 2013: Recommendations from CDC and the Q Fever Working Group","volume":"62","author":"Anderson","year":"2013","journal-title":"MMWR. Recomm. Rep."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1017\/S0950268899003763","article-title":"Long-Term Persistence of Coxiella burnetii in the Host after Primary Q Fever","volume":"124","author":"Harris","year":"2000","journal-title":"Epidemiol. Infect."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1086\/321889","article-title":"Risks Factors and Prevention of Q Fever Endocarditis","volume":"33","author":"Fenollar","year":"2001","journal-title":"Clin. Infect. Dis."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1429","DOI":"10.3201\/eid2108.140343","article-title":"Bartonella spp. and Coxiella burnetii Associated with Community-Acquired, Culture-Negative Endocarditis, Brazil","volume":"21","author":"Siciliano","year":"2015","journal-title":"Emerg. Infect. Dis."}],"container-title":["Tropical Medicine and Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2414-6366\/9\/4\/78\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:24:06Z","timestamp":1760106246000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2414-6366\/9\/4\/78"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,6]]},"references-count":41,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2024,4]]}},"alternative-id":["tropicalmed9040078"],"URL":"https:\/\/doi.org\/10.3390\/tropicalmed9040078","relation":{},"ISSN":["2414-6366"],"issn-type":[{"value":"2414-6366","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,6]]}}}