{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:03:05Z","timestamp":1760144585093,"version":"build-2065373602"},"reference-count":52,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2024,4,22]],"date-time":"2024-04-22T00:00:00Z","timestamp":1713744000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior (CAPES), Brazil","award":["001","CNE-E-26\/201.032\/2021","305956\/2021-3","316975\/2023-0"],"award-info":[{"award-number":["001","CNE-E-26\/201.032\/2021","305956\/2021-3","316975\/2023-0"]}]},{"name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro (FAPERJ), Brazil","award":["001","CNE-E-26\/201.032\/2021","305956\/2021-3","316975\/2023-0"],"award-info":[{"award-number":["001","CNE-E-26\/201.032\/2021","305956\/2021-3","316975\/2023-0"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq), Brazil","award":["001","CNE-E-26\/201.032\/2021","305956\/2021-3","316975\/2023-0"],"award-info":[{"award-number":["001","CNE-E-26\/201.032\/2021","305956\/2021-3","316975\/2023-0"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>The zoonotic visceral leishmaniasis is caused by the protozoan Leishmania infantum and dogs are reservoirs for this parasite. For the diagnosis of Leishmania at the species level in dogs in formalin-fixed, paraffin-embedded skin (FFPES) samples, colorimetric in situ hybridization (CISH) and quantitative real-time polymerase chain reaction (qPCR) are options, but their sensitivities are not well established. Therefore, the aim of this study was to determine the sensitivity of these two techniques in FFPES for the diagnosis of the L. infantum infection in dogs using culture as the reference standard. The FFPES of 48 dogs with cutaneous infection by L. infantum confirmed by culture and by multilocus enzyme electrophoresis were examined by CISH and qPCR using specific probes for L. infantum. The sensitivities of qPCR, CISH and their combination were, respectively, 77.0%, 58.0% and 83.3%. The sensitivities of qPCR in dogs with and without clinical signs were, respectively, 74.2% and 82.4%. The sensitivities of CISH in dogs with and without clinical signs were, respectively, 61.3% and 52.9%. The CISH and qPCR showed satisfactory sensitivities for the diagnosis of L. infantum in the FFPES of dogs, even in dogs without clinical signs, and their combination increases the sensitivity for this diagnosis.<\/jats:p>","DOI":"10.3390\/tropicalmed9040091","type":"journal-article","created":{"date-parts":[[2024,4,22]],"date-time":"2024-04-22T12:38:41Z","timestamp":1713789521000},"page":"91","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative Study of Colorimetric In Situ Hybridization and Quantitative Real-Time Polymerase Chain Reaction for Diagnosis of Infection by Leishmania infantum in Dogs in Formalin-Fixed and Paraffin-Embedded Skin"],"prefix":"10.3390","volume":"9","author":[{"given":"Ricardo Gon\u00e7alves","family":"Silva","sequence":"first","affiliation":[{"name":"Laboratory of Clinical Research on Dermatozoonoses in Domestic Animals, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Av. Brasil, 4365, Rio de Janeiro 21040-360, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4429-9529","authenticated-orcid":false,"given":"Matti","family":"Kiupel","sequence":"additional","affiliation":[{"name":"Veterinary Diagnostic Laboratory, Michigan State University, 4125 Beaumont Road, Lansing, MI 48910, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7366-4391","authenticated-orcid":false,"given":"Ingeborg Maria","family":"Langohr","sequence":"additional","affiliation":[{"name":"Sanofi, Global Discovery Pathology, Translational Models Research Platform, 350 Water Street, Cambridge, MA 02141, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annabel","family":"Wise","sequence":"additional","affiliation":[{"name":"Veterinary Diagnostic Laboratory, Michigan State University, 4125 Beaumont Road, Lansing, MI 48910, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0614-4713","authenticated-orcid":false,"given":"Sandro Antonio","family":"Pereira","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Research on Dermatozoonoses in Domestic Animals, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Av. Brasil, 4365, Rio de Janeiro 21040-360, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nat\u00e1lia Pedra","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Bio-Manguinhos, Oswaldo Cruz Foundation, Av. Brasil, 4365, Rio de Janeiro 21040-360, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Greice Maria Silva","family":"da Concei\u00e7\u00e3o","sequence":"additional","affiliation":[{"name":"Bio-Manguinhos, Oswaldo Cruz Foundation, Av. Brasil, 4365, Rio de Janeiro 21040-360, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luiz Cl\u00e1udio","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Research on Dermatozoonoses in Domestic Animals, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Av. Brasil, 4365, Rio de Janeiro 21040-360, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monique Paiva","family":"de Campos","sequence":"additional","affiliation":[{"name":"Carlos Chagas Institute, Oswaldo Cruz Foundation, Rua Prof. Algacyr Munhoz Mader, 3775, Curitiba 81350-010, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luciana de Freitas Campos","family":"Miranda","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Research and Surveillance of Leishmaniasis, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Av. Brasil, 4365, Rio de Janeiro 21040-360, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabiano Borges","family":"Figueiredo","sequence":"additional","affiliation":[{"name":"Carlos Chagas Institute, Oswaldo Cruz Foundation, Rua Prof. Algacyr Munhoz Mader, 3775, Curitiba 81350-010, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9387-8645","authenticated-orcid":false,"given":"Raquel de Vasconcellos Carvalhaes","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Epidemiology, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Av. 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