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The present work is characterized as a descriptive study in two areas: a forest area located in the Parque Estadual do Rio Doce, and another urban area located in the municipality of Tim\u00f3teo-MG, with the objective of identifying the presence of Leishmania spp. and the blood source of the collected female sand flies. Part of the females were obtained from the Parque Estadual do Rio Doce, and part was collected using 19 ligth traps distributed in residences of Tim\u00f3teo. For molecular studies of Leishmania spp. DNA, the ITS1 gene was used, and in the search for blood source, the CytB gene was used and positive samples were sequenced. The study demonstrated that there are at least three species of Leishmania circulating in the study areas: Leishmania (Viannia) braziliensis, Leishmania (Leishmania) amazonensis, and Leishmania (V.) guyanensis. Nyssomyia whitmani was the predominant sand fly species in the urban area of Tim\u00f3teo with a positive diagnosis for the presence of Leishmania braziliensis DNA. We found the presence of blood from Gallus gallus (Chicken) and Sus scrofa (Pig) in sand flies. The present study demonstrates that Leishmania braziliensis is the main agent of cutaneous leishmaniasis in the study area, with the effective participation of Nyssomyia whitmani as the vector and both Gallus gallus and Sus scrofa acting as a food source for female sand flies, and helping maintaining the sand fly life.<\/jats:p>","DOI":"10.3390\/tropicalmed9060133","type":"journal-article","created":{"date-parts":[[2024,6,13]],"date-time":"2024-06-13T06:23:11Z","timestamp":1718259791000},"page":"133","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Molecular Detection of Leishmania spp. and Blood Source of Female Sand Flies in the Parque Estadual do Rio Doce and Municipality of Tim\u00f3teo, Minas Gerais, Brazil"],"prefix":"10.3390","volume":"9","author":[{"given":"Cristian Ferreira de","family":"Souza","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Doen\u00e7as Parasit\u00e1rias, Instituto Oswaldo Cruz (Fiocruz), Avenida Brasil, 4365, Manguinhos, Rio de Janeiro 21040-360, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos Alberto dos","family":"Santos","sequence":"additional","affiliation":[{"name":"Centro de Controle de Zoonoses, Prefeitura Municipal de Tim\u00f3teo, Avenida Acesita, 3230, S\u00e3o Jos\u00e9, Tim\u00f3teo 35182-000, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0015-2154","authenticated-orcid":false,"given":"Paula Dias","family":"Bevilacqua","sequence":"additional","affiliation":[{"name":"Grupo de Pesquisa Viol\u00eancias, G\u00eanero e Sa\u00fade, Instituto Ren\u00e9 Rachou (Fiocruz), Avenida Augusto de Lima, 1715, Barro Preto, Belo Horizonte 30190-002, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9754-8464","authenticated-orcid":false,"given":"Jos\u00e9 Dilermando","family":"Andrade Filho","sequence":"additional","affiliation":[{"name":"Grupo de Estudos em Leishmanioses, Instituto Ren\u00e9 Rachou (Fiocruz), Avenida Augusto de Lima, 1715, Barro Preto, Belo Horizonte 30190-002, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reginaldo Pe\u00e7anha","family":"Brazil","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Doen\u00e7as Parasit\u00e1rias, Instituto Oswaldo Cruz (Fiocruz), Avenida Brasil, 4365, Manguinhos, Rio de Janeiro 21040-360, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,13]]},"reference":[{"key":"ref_1","unstructured":"Chang, K.P., and Bray, R.S. 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