{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T00:59:40Z","timestamp":1780448380761,"version":"3.54.1"},"reference-count":27,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2024,6,20]],"date-time":"2024-06-20T00:00:00Z","timestamp":1718841600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100013115","name":"Zhejiang Provincial Program for the Cultivation of High-level Innovative Health Talents","doi-asserted-by":"publisher","award":["WJW2021002"],"award-info":[{"award-number":["WJW2021002"]}],"id":[{"id":"10.13039\/501100013115","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013115","name":"Zhejiang Provincial Program for the Cultivation of High-level Innovative Health Talents","doi-asserted-by":"publisher","award":["2020WZK2003"],"award-info":[{"award-number":["2020WZK2003"]}],"id":[{"id":"10.13039\/501100013115","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013115","name":"Zhejiang Provincial Program for the Cultivation of High-level Innovative Health Talents","doi-asserted-by":"publisher","award":["2021WZK1003"],"award-info":[{"award-number":["2021WZK1003"]}],"id":[{"id":"10.13039\/501100013115","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013115","name":"Zhejiang Provincial Program for the Cultivation of High-level Innovative Health Talents","doi-asserted-by":"publisher","award":["2022C03109"],"award-info":[{"award-number":["2022C03109"]}],"id":[{"id":"10.13039\/501100013115","id-type":"DOI","asserted-by":"publisher"}]},{"name":"NHC Key Laboratory of Echinococcosis Prevention and Control","award":["WJW2021002"],"award-info":[{"award-number":["WJW2021002"]}]},{"name":"NHC Key Laboratory of Echinococcosis Prevention and Control","award":["2020WZK2003"],"award-info":[{"award-number":["2020WZK2003"]}]},{"name":"NHC Key Laboratory of Echinococcosis Prevention and Control","award":["2021WZK1003"],"award-info":[{"award-number":["2021WZK1003"]}]},{"name":"NHC Key Laboratory of Echinococcosis Prevention and Control","award":["2022C03109"],"award-info":[{"award-number":["2022C03109"]}]},{"name":"Key R&amp;D projects of Zhejiang province","award":["WJW2021002"],"award-info":[{"award-number":["WJW2021002"]}]},{"name":"Key R&amp;D projects of Zhejiang province","award":["2020WZK2003"],"award-info":[{"award-number":["2020WZK2003"]}]},{"name":"Key R&amp;D projects of Zhejiang province","award":["2021WZK1003"],"award-info":[{"award-number":["2021WZK1003"]}]},{"name":"Key R&amp;D projects of Zhejiang province","award":["2022C03109"],"award-info":[{"award-number":["2022C03109"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>Echinococcosis poses a significant concern in the fields of public health and veterinary care as it can be transmitted between animals and humans. The primary endemic subtypes are cystic echinococcosis (CE) and alveolar echinococcosis (AE), which result from infestation by Echinococcus granulosus and Echinococcus multilocularis, respectively. A prominent epidemic of echinococcosis greatly affects the Tibet Autonomous Region (TAR) in China. A new technique called the loop-mediated isothermal amplification\u2013lateral flow dipstick (LAMP-LFD) test is introduced in this research to differentiate between E. granulosus and E. multilocularis using their repetitive genetic sequences. The test is characterized by its portable nature, simple operation, quick result production, high sensitivity, and low susceptibility to aerosol contamination. The LAMP-LFD method demonstrated an exceptional minimal detection limit, reaching levels as low as approximately 1 fg\/\u03bcL (femtogram per microliter) of genomic DNA. The assay\u2019s specificity was assessed, and no cross-reactivity was seen. A total of 982 dog fecal samples were collected from 54 counties in the TAR region between July 2021 and June 2022. The established method underwent validation using a commercially available ELISA kit. The agreement rate between the LAMP-LFD and ELISA methods was 97.25%, with a sensitivity of 96.05% and a specificity of 97.35%. The assay described in this study improves specificity by using a double-labeled probe, and it reduces the risk of false-positive results caused by aerosol contamination through the use of a sealed device. This makes it a suitable choice for quickly and accurately identifying the two main types of Echinococcus in field settings.<\/jats:p>","DOI":"10.3390\/tropicalmed9060136","type":"journal-article","created":{"date-parts":[[2024,6,20]],"date-time":"2024-06-20T03:46:29Z","timestamp":1718855189000},"page":"136","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Rapid Discriminative Identification of the Two Predominant Echinococcus Species from Canine Fecal Samples in the Tibetan Region of China by Loop-Mediated Isothermal Amplification\u2013Lateral Flow Dipstick Assay"],"prefix":"10.3390","volume":"9","author":[{"given":"Xinyue","family":"Lv","sequence":"first","affiliation":[{"name":"Key Laboratory of Bio-Tech Vaccine of Zhejiang Province, Engineering Research Center of Novel Vaccine of Zhejiang Province, School of Basic Medicine and Forensics, Hangzhou Medical College, Hangzhou 310013, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiajia","family":"Ai","sequence":"additional","affiliation":[{"name":"Tibet Center for Disease Control and Prevention, NHC Key Laboratory of Echinococcosis Prevention and Control, Lhasa 850000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3510-717X","authenticated-orcid":false,"given":"Xiaojin","family":"Mo","sequence":"additional","affiliation":[{"name":"Tibet Center for Disease Control and Prevention, NHC Key Laboratory of Echinococcosis Prevention and Control, Lhasa 850000, China"},{"name":"National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Chinese Center for Tropical Diseases Research, National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, Key Laboratory on Parasite and Vector Biology, Ministry of Health, Shanghai 200025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haojie","family":"Ding","sequence":"additional","affiliation":[{"name":"Key Laboratory of Bio-Tech Vaccine of Zhejiang Province, Engineering Research Center of Novel Vaccine of Zhejiang Province, School of Basic Medicine and Forensics, Hangzhou Medical College, Hangzhou 310013, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofia","family":"Litchev","sequence":"additional","affiliation":[{"name":"Department of Chemistry & Biochemistry, University of California, Los Angeles, CA 90095, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Entung","family":"Lu","sequence":"additional","affiliation":[{"name":"Santa Monica College, Los Angeles, CA 90405, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youhong","family":"Weng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Bio-Tech Vaccine of Zhejiang Province, Engineering Research Center of Novel Vaccine of Zhejiang Province, School of Basic Medicine and Forensics, Hangzhou Medical College, Hangzhou 310013, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"He","sequence":"additional","affiliation":[{"name":"Key Laboratory of Bio-Tech Vaccine of Zhejiang Province, Engineering Research Center of Novel Vaccine of Zhejiang Province, School of Basic Medicine and Forensics, Hangzhou Medical College, Hangzhou 310013, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quzhen","family":"Gongsang","sequence":"additional","affiliation":[{"name":"Tibet Center for Disease Control and Prevention, NHC Key Laboratory of Echinococcosis Prevention and Control, Lhasa 850000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3452-3638","authenticated-orcid":false,"given":"Shijie","family":"Yang","sequence":"additional","affiliation":[{"name":"National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Chinese Center for Tropical Diseases Research, National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, Key Laboratory on Parasite and Vector Biology, Ministry of Health, Shanghai 200025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiumin","family":"Ma","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Clinical Laboratory Center, Tumor Hospital Affiliated to Xinjiang Medical University, Urumqi 830000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingzhong","family":"Li","sequence":"additional","affiliation":[{"name":"Tibet Center for Disease Control and Prevention, NHC Key Laboratory of Echinococcosis Prevention and Control, Lhasa 850000, 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