{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T01:42:50Z","timestamp":1788486170499,"version":"build-2803163510"},"reference-count":64,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,7,25]],"date-time":"2024-07-25T00:00:00Z","timestamp":1721865600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"GIDRN\u2014Global Infectious Disease Research Network","award":["111584467514"],"award-info":[{"award-number":["111584467514"]}]},{"name":"GIDRN\u2014Global Infectious Disease Research Network","award":["D43-TW010331"],"award-info":[{"award-number":["D43-TW010331"]}]},{"name":"Minciencias","award":["111584467514"],"award-info":[{"award-number":["111584467514"]}]},{"name":"Minciencias","award":["D43-TW010331"],"award-info":[{"award-number":["D43-TW010331"]}]},{"name":"Fogarty program","award":["111584467514"],"award-info":[{"award-number":["111584467514"]}]},{"name":"Fogarty program","award":["D43-TW010331"],"award-info":[{"award-number":["D43-TW010331"]}]},{"name":"Academic Vice-Rector\u2019s Office of the \u201cPontificia Universidad Javeriana\u201d","award":["111584467514"],"award-info":[{"award-number":["111584467514"]}]},{"name":"Academic Vice-Rector\u2019s Office of the \u201cPontificia Universidad Javeriana\u201d","award":["D43-TW010331"],"award-info":[{"award-number":["D43-TW010331"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>Leptospira is a bacterial genus that includes several pathogenic species related to leptospirosis. In Colombia, leptospirosis is a mandatorily reported disease, widely distributed across the country. In the Villeta municipality, leptospirosis has been identified as an important cause of febrile illness; however, to date, no studies have been performed to identify the circulating species. A genus-specific qualitative qPCR was performed on DNA extracted from febrile patients\u2019 acute-phase whole-blood samples targeting a fragment of the rrs gene. Positive qPCR samples were further amplified for the adk, icdA, LipL32, LipL41, rrs, and secY genes through conventional PCR for sequencing. All high-quality obtained sequences were further assessed through concatenated phylogenetic analysis. A total of 25% (14\/56) of febrile patients\u2019 acute blood samples were positive for Leptospira spp. High-quality sequences were obtained for only five genes, and analysis through concatenated phylogeny identified that all sequences clustered within the P1\/pathogenic clade; some of them formed a robustly supported clade with Leptospira\u00a0santarosai, and others were closely related with other Leptospira species but exhibited considerable genetic divergence. We describe the presence of pathogenic Leptospira species among febrile patients from the Villeta municipality and identify L. santarosai and other Leptospira species as causative agents of leptospirosis in the region.<\/jats:p>","DOI":"10.3390\/tropicalmed9080168","type":"journal-article","created":{"date-parts":[[2024,7,25]],"date-time":"2024-07-25T08:40:04Z","timestamp":1721896804000},"page":"168","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Molecular Characterization of Leptospira Species among Patients with Acute Undifferentiated Febrile Illness from the Municipality of Villeta, Colombia"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0669-494X","authenticated-orcid":false,"given":"Carlos Ramiro","family":"Silva-Ramos","sequence":"first","affiliation":[{"name":"Grupo de Enfermedades Infecciosas, Departamento de Microbiolog\u00eda, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogot\u00e1 110231, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7715-8541","authenticated-orcid":false,"given":"J. Manuel","family":"Matiz-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Grupo de Enfermedades Infecciosas, Departamento de Microbiolog\u00eda, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogot\u00e1 110231, Colombia"},{"name":"Molecular Genetics and Antimicrobial Resistance Unit, Universidad El Bosque, Bogot\u00e1 110121, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana","family":"Gil-Mora","sequence":"additional","affiliation":[{"name":"Grupo de Enfermedades Infecciosas, Departamento de Microbiolog\u00eda, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogot\u00e1 110231, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7239-6428","authenticated-orcid":false,"given":"Heidy-C.","family":"Mart\u00ednez D\u00edaz","sequence":"additional","affiliation":[{"name":"Grupo de Enfermedades Infecciosas, Departamento de Microbiolog\u00eda, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogot\u00e1 110231, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1127-0132","authenticated-orcid":false,"given":"\u00c1lvaro A.","family":"Faccini-Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Servicio de Infectolog\u00eda, Hospital Militar Central, Bogot\u00e1 110110, Colombia"},{"name":"Facultad de Medicina, Universidad Militar Nueva Granada, Bogot\u00e1 110111, Colombia"},{"name":"Servicios y Asesor\u00edas en Infectolog\u00eda\u2014SAI, Bogot\u00e1 110110, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3494-6481","authenticated-orcid":false,"given":"Claudia","family":"Cuervo","sequence":"additional","affiliation":[{"name":"Grupo de Enfermedades Infecciosas, Departamento de Microbiolog\u00eda, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogot\u00e1 110231, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter C.","family":"Melby","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal Medicine, University of Texas Medical Branch, Galveston, TX 77555, USA"},{"name":"Center for Tropical Diseases, University of Texas Medical Branch, Galveston, TX 77555, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia V.","family":"Aguilar","sequence":"additional","affiliation":[{"name":"Center for Tropical Diseases, University of Texas Medical Branch, Galveston, TX 77555, USA"},{"name":"Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2545-6197","authenticated-orcid":false,"given":"Miguel M.","family":"Cabada","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal Medicine, University of Texas Medical Branch, Galveston, TX 77555, USA"},{"name":"Center for Tropical Diseases, University of Texas Medical Branch, Galveston, TX 77555, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7054-9918","authenticated-orcid":false,"given":"Juan David","family":"Rodas","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Ciencias Veterinarias Centauro, Universidad de Antioquia, Medell\u00edn 050010, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marylin","family":"Hidalgo","sequence":"additional","affiliation":[{"name":"Grupo de Enfermedades Infecciosas, Departamento de Microbiolog\u00eda, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogot\u00e1 110231, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.vetmic.2009.03.012","article-title":"Leptospira and leptospirosis","volume":"140","author":"Adler","year":"2010","journal-title":"Vet. 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