{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T19:44:00Z","timestamp":1767383040659,"version":"build-2065373602"},"reference-count":35,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2024,8,25]],"date-time":"2024-08-25T00:00:00Z","timestamp":1724544000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Foundation for the Promotion of Health and Biomedical Research of the Valencian Community (FISABIO)","award":["UGP-19-031"],"award-info":[{"award-number":["UGP-19-031"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>Strongyloidiasis is a widespread parasitic disease that can be life-threatening in immunosuppressed people. In the Mediterranean basin, autochthonous cases coexist with imported ones. We aimed to assess the utility of different screening methods, along with the frequency of strongyloidiasis and its associated risk factors in migrants and the native population. This cross-sectional study took place from 2019 to 2022 in the area of the Vega Baja Hospital in Alicante, Spain. Screening was performed in people who were immunosuppressed, at risk of immunosuppression, with blood asymptomatic eosinophilia, and in asymptomatic people from highly endemic countries. Screening methods were serological techniques (ELISA), stool parasitological tests (fecal concentration methods and agar plate culture), and a stool molecular test (PCR). Of the 168 participants (62.5% males, 53.0% migrants, 36.3% immunosuppressed, median age 57 years), 14 (8.3%) had confirmed strongyloidiasis, where 6 were confirmed by serology, 4 by PCR, and 4 by both methods. Overall, 9% of the migrants and 7.6% of the native-born patients were infected. Elevated IgE and hemoglobin and Latin American origin were associated with strongyloidiasis diagnosis. Screening with serology alone would have missed 28.6% of cases. We conclude that strongyloidiasis prevalence is high in our population, both in native and migrant groups, and stool PCR is a useful tool to increase case detection.<\/jats:p>","DOI":"10.3390\/tropicalmed9090194","type":"journal-article","created":{"date-parts":[[2024,8,26]],"date-time":"2024-08-26T01:48:49Z","timestamp":1724636929000},"page":"194","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Concomitant Serological and Molecular Methods for Strongyloides stercoralis Screening in an Endemic Area of Spain"],"prefix":"10.3390","volume":"9","author":[{"given":"Ana","family":"Lucas Dato","sequence":"first","affiliation":[{"name":"Internal Medicine\/Infectious Diseases Department, Vega Baja Hospital-Orihuela, 03314 Alicante, Spain"},{"name":"Foundation for the Promotion of Health and Biomedical Research of the Valencia Region (FISABIO), 46020 Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6329-0311","authenticated-orcid":false,"given":"Philp","family":"Wikman-Jorgensen","sequence":"additional","affiliation":[{"name":"Foundation for the Promotion of Health and Biomedical Research of the Valencia Region (FISABIO), 46020 Valencia, Spain"},{"name":"Internal Medicine\/Infectious Diseases Department, Elda General University Hospital, 03600 Alicante, Spain"},{"name":"Department of Clinical Medicine, Miguel Hernandez University of Elche, 03202 Elche, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emilio","family":"Borrajo Brunete","sequence":"additional","affiliation":[{"name":"Microbiology Department, Vega Baja Hospital-Orihuela, 03314 Alicante, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Dolores","family":"Hern\u00e1ndez Rabad\u00e1n","sequence":"additional","affiliation":[{"name":"Internal Medicine\/Infectious Diseases Department, Vega Baja Hospital-Orihuela, 03314 Alicante, Spain"},{"name":"Foundation for the Promotion of Health and Biomedical Research of the Valencia Region (FISABIO), 46020 Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hilari\u00f3n","family":"Garc\u00eda-Morante","sequence":"additional","affiliation":[{"name":"Internal Medicine\/Infectious Diseases Department, Vega Baja Hospital-Orihuela, 03314 Alicante, Spain"},{"name":"Foundation for the Promotion of Health and Biomedical Research of the Valencia Region (FISABIO), 46020 Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Adelino","family":"Merino Trigueros","sequence":"additional","affiliation":[{"name":"Internal Medicine\/Infectious Diseases Department, Vega Baja Hospital-Orihuela, 03314 Alicante, Spain"},{"name":"Foundation for the Promotion of Health and Biomedical Research of the Valencia Region (FISABIO), 46020 Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8950-5766","authenticated-orcid":false,"given":"Jos\u00e9 Mar\u00eda","family":"Saugar Cruz","sequence":"additional","affiliation":[{"name":"Laboratory of Reference and Research in Parasitology, Centro Nacional de Microbiolog\u00eda, Instituto de Salud Carlos III, 28222 Majadahonda, Spain"},{"name":"Biomedical Research Networking Center of Infectious Diseases (CIBERINFEC), Carlos III Institute, 28029 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisa","family":"Garc\u00eda-Vazquez","sequence":"additional","affiliation":[{"name":"Infectious Diseases Unit, Hospital Virgen de la Arrixaca, 30120 Murcia, Spain"},{"name":"Medicine Department, University of Murcia, 30003 Murcia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9125-7706","authenticated-orcid":false,"given":"Jara","family":"Llenas-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Internal Medicine\/Infectious Diseases Department, Vega Baja Hospital-Orihuela, 03314 Alicante, Spain"},{"name":"Foundation for the Promotion of Health and Biomedical Research of the Valencia Region (FISABIO), 46020 Valencia, Spain"},{"name":"Department of Clinical Medicine, Miguel Hernandez University of Elche, 03202 Elche, Spain"},{"name":"Biomedical Research Networking Center of Infectious Diseases (CIBERINFEC), Carlos III Institute, 28029 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1016\/j.trstmh.2009.02.013","article-title":"Strongyloidiasis\u2014The most neglected of the neglected tropical diseases?","volume":"103","author":"Olsen","year":"2009","journal-title":"Trans. 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