{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T02:01:43Z","timestamp":1783735303211,"version":"3.55.0"},"reference-count":46,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2024,9,2]],"date-time":"2024-09-02T00:00:00Z","timestamp":1725235200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","award":["001"],"award-info":[{"award-number":["001"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","award":["APQ-02760-17"],"award-info":[{"award-number":["APQ-02760-17"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","award":["U01 AI151698"],"award-info":[{"award-number":["U01 AI151698"]}]},{"name":"FAPEMIG (Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais)","award":["001"],"award-info":[{"award-number":["001"]}]},{"name":"FAPEMIG (Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais)","award":["APQ-02760-17"],"award-info":[{"award-number":["APQ-02760-17"]}]},{"name":"FAPEMIG (Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais)","award":["U01 AI151698"],"award-info":[{"award-number":["U01 AI151698"]}]},{"name":"CNPq","award":["001"],"award-info":[{"award-number":["001"]}]},{"name":"CNPq","award":["APQ-02760-17"],"award-info":[{"award-number":["APQ-02760-17"]}]},{"name":"CNPq","award":["U01 AI151698"],"award-info":[{"award-number":["U01 AI151698"]}]},{"name":"Brazilian Ministry of Health","award":["001"],"award-info":[{"award-number":["001"]}]},{"name":"Brazilian Ministry of Health","award":["APQ-02760-17"],"award-info":[{"award-number":["APQ-02760-17"]}]},{"name":"Brazilian Ministry of Health","award":["U01 AI151698"],"award-info":[{"award-number":["U01 AI151698"]}]},{"name":"National Institutes of Health USA","award":["001"],"award-info":[{"award-number":["001"]}]},{"name":"National Institutes of Health USA","award":["APQ-02760-17"],"award-info":[{"award-number":["APQ-02760-17"]}]},{"name":"National Institutes of Health USA","award":["U01 AI151698"],"award-info":[{"award-number":["U01 AI151698"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>West Nile Virus (WNV) poses a significant global public health threat as a mosquito-borne pathogen. While laboratory mouse models have historically played a crucial role in understanding virus biology, recent research has focused on utilizing immunocompromised models to study arboviruses like dengue and Zika viruses, particularly their interactions with Aedes aegypti mosquitoes. However, there has been a shortage of suitable mouse models for investigating WNV and St. Louis encephalitis virus interactions with their primary vectors, Culex spp. mosquitoes. Here, we establish the AG129 mouse (IFN \u03b1\/\u03b2\/\u03b3 R\u2212\/\u2212) as an effective vertebrate model for examining mosquito\u2013WNV interactions. Following intraperitoneal injection, AG129 mice exhibited transient viremia lasting several days, peaking on the second or third day post-infection, which is sufficient to infect Culex quinquefasciatus mosquitoes during a blood meal. We also observed WNV replication in the midgut and dissemination to other tissues, including the fat body, in infected mosquitoes. Notably, infectious virions were present in the saliva of a viremic AG129 mouse 16 days post-exposure, indicating successful transmission capacity. These findings highlight the utility of AG129 mice for studying vector competence and WNV\u2013mosquito interactions.<\/jats:p>","DOI":"10.3390\/tropicalmed9090201","type":"journal-article","created":{"date-parts":[[2024,9,2]],"date-time":"2024-09-02T07:59:40Z","timestamp":1725263980000},"page":"201","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Suitable Mouse Model to Study Dynamics of West Nile Virus Infection in Culex quinquefasciatus Mosquitoes"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-1953-7775","authenticated-orcid":false,"given":"L\u00edvia","family":"Baldon","sequence":"first","affiliation":[{"name":"Mosquitos Vetores: Endossimbiontes e Intera\u00e7\u00e3o Pat\u00f3geno-Vetor, Instituto Ren\u00e9 Rachou-Fiocruz, Belo Horizonte 30190-002, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvana","family":"de Mendon\u00e7a","sequence":"additional","affiliation":[{"name":"Mosquitos Vetores: Endossimbiontes e Intera\u00e7\u00e3o Pat\u00f3geno-Vetor, Instituto Ren\u00e9 Rachou-Fiocruz, Belo Horizonte 30190-002, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen","family":"Santos","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica e Imunologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, 6627-Pampulha, Belo Horizonte 31270-901, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Mar\u00e7al","sequence":"additional","affiliation":[{"name":"Mosquitos Vetores: Endossimbiontes e Intera\u00e7\u00e3o Pat\u00f3geno-Vetor, Instituto Ren\u00e9 Rachou-Fiocruz, Belo Horizonte 30190-002, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8600-4077","authenticated-orcid":false,"given":"Amanda Cupertino","family":"de 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