{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T01:54:16Z","timestamp":1792806856851,"version":"4.5.0"},"reference-count":25,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2024,10,4]],"date-time":"2024-10-04T00:00:00Z","timestamp":1728000000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior\u2014Brasil (CAPES)","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"https:\/\/ror.org\/00x0ma614","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>Leptospirosis is an infectious disease caused by the pathogenic Leptospira species through direct or indirect contact with infected animals. Due to protean clinical manifestation in the early stages, leptospirosis is often difficult to distinguish from other common acute febrile illnesses, such as dengue. Thus, this study aimed to investigate the prevalence of leptospirosis in suspected dengue patients whose serological diagnosis was negative. A total of 449 serum samples from patients (negative IgM-ELISA dengue) with fever, headache, myalgia, and nausea were tested. The Dual-Path Platform (DPP) rapid test developed by the Instituto de Tecnologia em Imunobiol\u00f3gicos Bio-Manguinhos in the city of Rio de Janeiro, Brazil was used for screening IgM antibodies against Leptospira in blood serum, and the microscopic agglutination test (MAT) was performed on samples positive in the DPP for leptospirosis, as well as on an equal number of negative samples. Results: The data obtained from the samples analyzed with the DPP assay showed 26 positive results (5.79%), of which 38.46% were male and 61.54% female, with a mean age of 41 years. We tested 52 samples using the MAT, including 26 reactive for IgM and 26 non-reactive in the DPP assay. Nine samples (17.31%) were reactive, and among them, six also showed reactivity in the DPP assay. Of the six samples reactive in both tests, 66.67% were female, living in urban areas in the city of Uberl\u00e2ndia, with a mean age of 50 years, being 50% white, 33.33% brown, and 16.67% black. The findings demonstrated that leptospirosis cases are underdiagnosed and undertreated in the study population and more attention needs to be paid for ruling out leptospirosis and other pathogens causing acute febrile illness in dengue-endemic areas.<\/jats:p>","DOI":"10.3390\/tropicalmed9100229","type":"journal-article","created":{"date-parts":[[2024,10,4]],"date-time":"2024-10-04T12:02:20Z","timestamp":1728043340000},"page":"229","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Under-Reporting of Human Leptospirosis Cases in Cities of Tri\u00e2ngulo Mineiro, Minas Gerais, Brazil"],"prefix":"10.3390","volume":"9","author":[{"given":"Mariani Borges","family":"Franco","sequence":"first","affiliation":[{"name":"Graduate Program in Health Sciences, Federal University of Uberl\u00e2ndia, Uberl\u00e2ndia 38400-902, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lara Reis","family":"Gomes","sequence":"additional","affiliation":[{"name":"Laboratory of Contagious Infectious Diseases, Faculty of Veterinary Medicine, Federal University of Uberl\u00e2ndia, Uberl\u00e2ndia 38400-902, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Rostkwoska","sequence":"additional","affiliation":[{"name":"Laboratory of Immunoparasitology, Institute of Biomedical Sciences, Federal University of Uberl\u00e2ndia, Uberl\u00e2ndia 38400-902, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Cl\u00e1udia Arantes Marquez","family":"Pajuaba","sequence":"additional","affiliation":[{"name":"Laboratory of Immunoparasitology, Institute of Biomedical Sciences, Federal University of Uberl\u00e2ndia, Uberl\u00e2ndia 38400-902, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9010-7228","authenticated-orcid":false,"given":"Jos\u00e9 Roberto","family":"Mineo","sequence":"additional","affiliation":[{"name":"Laboratory of Immunoparasitology, Institute of Biomedical Sciences, Federal University of Uberl\u00e2ndia, Uberl\u00e2ndia 38400-902, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9567-3627","authenticated-orcid":false,"given":"Anna Monteiro Correia","family":"Lima","sequence":"additional","affiliation":[{"name":"Laboratory of Contagious Infectious Diseases, Faculty of Veterinary Medicine, Federal University of Uberl\u00e2ndia, Uberl\u00e2ndia 38400-902, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan Vilges de","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Collective Health, Faculty of Medicine, Federal University of Uberl\u00e2ndia, Uberl\u00e2ndia 38400-902, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"14","DOI":"10.7861\/clinmed.2021-0784","article-title":"Leptospirosis: Clinical aspects","volume":"22","author":"Rajapakse","year":"2022","journal-title":"Clin. 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