{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T01:17:18Z","timestamp":1788484638284,"version":"build-2803163510"},"reference-count":79,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2024,11,18]],"date-time":"2024-11-18T00:00:00Z","timestamp":1731888000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Population Health and Welfare Research Center from the Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education in Taiwan","award":["NTU-113L9004"],"award-info":[{"award-number":["NTU-113L9004"]}]},{"name":"Population Health and Welfare Research Center from the Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education in Taiwan","award":["11104000-0106-0000-2120"],"award-info":[{"award-number":["11104000-0106-0000-2120"]}]},{"name":"the Ministry of Interior, National Land Management Agency","award":["NTU-113L9004"],"award-info":[{"award-number":["NTU-113L9004"]}]},{"name":"the Ministry of Interior, National Land Management Agency","award":["11104000-0106-0000-2120"],"award-info":[{"award-number":["11104000-0106-0000-2120"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["TropicalMed"],"abstract":"<jats:p>Leptospirosis and hantavirus syndrome are two major rodent-borne diseases in Taiwan. Rocahepevirus ratii (RHEV), a virus closely related to hepatitis E virus (HEV, Paslahepevirus balayani), is emerging and has been reported to cause hepatitis in humans. We employed wastewater-based epidemiology to actively monitor rodent-borne pathogens, and the correlations with human cases were evaluated. Wastewater was collected using grab sampling at 11 sites along a sewer system including influents and effluents at a wastewater treatment plant in Tamsui, New Taipei City, Taiwan, monthly during June 2023 to May 2024. The presence of pathogens was examined by reverse transcription-polymerase chain reaction (RT-PCR). The result showed an overall positivity rate of 38.2% (50\/131). Leptospira was detected most often (48\/131, 36.6%), and RHEV and hantaviruses were found once each during the study period. Sequencing identified Leptospira interrogans close to isolates from rodents and human cases, while sequences of hantavirus and RHEV were most similar to isolates from rodents. No significant correlation was found with human cases or positive samples for rodent DNA. Here, we present an example of a One Health approach applying wastewater to environmental surveillance for the early detection and prevention of emerging diseases.<\/jats:p>","DOI":"10.3390\/tropicalmed9110282","type":"journal-article","created":{"date-parts":[[2024,11,18]],"date-time":"2024-11-18T07:45:27Z","timestamp":1731915927000},"page":"282","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Surveillance of Emerging Rodent-Borne Pathogens in Wastewater in Taiwan: A One Health Approach"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6154-113X","authenticated-orcid":false,"given":"Kun-Hsien","family":"Tsai","sequence":"first","affiliation":[{"name":"Institute of Environmental and Occupational Health Sciences, College of Public Health, National Taiwan University, Taipei 100025, Taiwan"},{"name":"Global Health Program, College of Public Health, National Taiwan University, Taipei 100025, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsai-Ying","family":"Yen","sequence":"additional","affiliation":[{"name":"Institute of Environmental and Occupational Health Sciences, College of Public Health, National Taiwan University, Taipei 100025, Taiwan"},{"name":"Center for Diagnostics and Vaccine Development, Centers for Disease Control, Ministry of Health and Welfare, Taipei 115201, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2137-2295","authenticated-orcid":false,"given":"Hsin-Hsin","family":"Tung","sequence":"additional","affiliation":[{"name":"Graduate Institute of Environmental Engineering, National Taiwan University, Taipei 106319, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy","family":"Ho","sequence":"additional","affiliation":[{"name":"Institute of Environmental and Occupational Health Sciences, College of Public Health, National Taiwan University, Taipei 100025, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang-Ta","family":"Chien","sequence":"additional","affiliation":[{"name":"Institute of Environmental and Occupational Health Sciences, College of Public Health, National Taiwan University, Taipei 100025, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chung-Yu","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Environmental and Occupational Health Sciences, College of Public Health, National Taiwan University, Taipei 100025, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu-Wei","family":"Kang","sequence":"additional","affiliation":[{"name":"Institute of Environmental and Occupational Health Sciences, College of Public Health, National Taiwan University, Taipei 100025, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning-Ning","family":"Juan","sequence":"additional","affiliation":[{"name":"Institute of Environmental and Occupational Health Sciences, College of Public Health, National Taiwan University, Taipei 100025, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang-Ling","family":"Lin","sequence":"additional","affiliation":[{"name":"Institute of Environmental and Occupational Health Sciences, College of Public Health, National Taiwan University, Taipei 100025, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,11,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1017\/S095026881000316X","article-title":"Role of environmental poliovirus surveillance in global polio eradication and beyond","volume":"140","author":"Hovi","year":"2012","journal-title":"Epidemiol. 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