{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T12:34:31Z","timestamp":1766493271000,"version":"build-2065373602"},"reference-count":53,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2018,2,6]],"date-time":"2018-02-06T00:00:00Z","timestamp":1517875200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["TRR127"],"award-info":[{"award-number":["TRR127"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>The porcine cytomegalovirus (PCMV) is a herpesvirus that may pose a risk for xenotransplantation using pig cells, tissues, or organs. Here, three orthotopic pig heart transplantations into baboons were studied. To detect PCMV, a real-time PCR and a Western blot assay based on four PCMV protein sequences, including two tegument proteins, were used. The transmission of PCMV from the donor pig to the recipient baboon was found in two cases, despite PCMV not being detected in the blood of the donor pigs by real-time PCR. Although it was not in the blood, PCMV was detected in different organs of the donor pigs, and in sibling animals. Immunohistochemistry using an antiserum that is specific for PCMV detected virus protein-expressing cells in all of the organs of the recipient baboon, most likely representing disseminated pig cells. Therefore, for the first time, the distribution of PCMV in organs of the donor pigs and the recipient baboons was described. In addition, baboon cytomegalovirus (BaCMV) was found activated in the recipient, and a screening for hepatitis E virus (HEV) and porcine lymphotropic herpesviruses (PLHV) was performed. For the first time, a cross-reactivity between antibodies directed against PCMV and BaCMV was found.<\/jats:p>","DOI":"10.3390\/v10020066","type":"journal-article","created":{"date-parts":[[2018,2,6]],"date-time":"2018-02-06T15:18:05Z","timestamp":1517930285000},"page":"66","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Distribution of Porcine Cytomegalovirus in Infected Donor Pigs and in Baboon Recipients of Pig Heart Transplantation"],"prefix":"10.3390","volume":"10","author":[{"given":"Uwe","family":"Fiebig","sequence":"first","affiliation":[{"name":"Department of HIV and other Retroviruses, Robert Koch Institute, 13535 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan-Michael","family":"Abicht","sequence":"additional","affiliation":[{"name":"Department of Anaesthesiology, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, 81377 Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tanja","family":"Mayr","sequence":"additional","affiliation":[{"name":"Department of Anaesthesiology, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, 81377 Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthias","family":"L\u00e4ngin","sequence":"additional","affiliation":[{"name":"Department of Anaesthesiology, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, 81377 Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8075-8222","authenticated-orcid":false,"given":"Andrea","family":"B\u00e4hr","sequence":"additional","affiliation":[{"name":"Molecular Animal Breeding and Biotechnology, Gene Center, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, 85764 Oberschlei\u00dfheim, Germany"},{"name":"Medizinische Klinik und Poliklinik, Klinikum Rechts der Isar, Technische Universit\u00e4t M\u00fcnchen, 81675 Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sonja","family":"Guethoff","sequence":"additional","affiliation":[{"name":"Walter Brendel Centre of Experimental Medicine, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, 81377 Munich, Germany"},{"name":"Department of Cardiac Surgery, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, 81377 Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Almuth","family":"Falkenau","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Pathology at the Centre for Clinical Veterinary Medicine, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, 81377 Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eckhard","family":"Wolf","sequence":"additional","affiliation":[{"name":"Molecular Animal Breeding and Biotechnology, Gene Center, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, 85764 Oberschlei\u00dfheim, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno","family":"Reichart","sequence":"additional","affiliation":[{"name":"Walter Brendel Centre of Experimental Medicine, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, 81377 Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9335-5596","authenticated-orcid":false,"given":"Tomoyuki","family":"Shibahara","sequence":"additional","affiliation":[{"name":"Pathology and Pathophysiology Research Division, National Institute of Animal Health, Tsukuba 305-0856, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3244-6085","authenticated-orcid":false,"given":"Joachim","family":"Denner","sequence":"additional","affiliation":[{"name":"Robert Koch Fellow, Robert Koch Institute, 13353 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2018,2,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1016\/S0140-6736(11)61091-X","article-title":"Clinical xenotransplantation, the next medical revolution?","volume":"379","author":"Ekser","year":"2012","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1136","DOI":"10.1002\/lt.24461","article-title":"Liver transplantation in Germany","volume":"22","author":"Tacke","year":"2016","journal-title":"Liver Transplant."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1007\/s11248-016-9934-8","article-title":"The production of multi-transgenic pigs, update and perspectives for xenotransplantation","volume":"25","author":"Niemann","year":"2016","journal-title":"Transgen. 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