{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T22:44:42Z","timestamp":1788561882865,"version":"build-2803163510"},"reference-count":45,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2018,3,6]],"date-time":"2018-03-06T00:00:00Z","timestamp":1520294400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Administrative Department of Science, Technology, and Innovation (COLCIENCIAS) and the Instituto Nacional de Salud (INS)","award":["210465740573"],"award-info":[{"award-number":["210465740573"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Rabies is a viral infection that targets the nervous system, specifically neurons. The clinical manifestations of the disease are dramatic and their outcome fatal; paradoxically, conventional histopathological descriptions reveal only subtle changes in the affected nervous tissue. Some researchers have considered that the pathophysiology of rabies is based more on biochemical changes than on structural alterations, as is the case with some psychiatric diseases. However, we believe that it has been necessary to resort to other methods that allow us to analyze the effect of the infection on neurons. The Golgi technique is the gold standard for studying the morphology of all the components of a neuron and the cytoskeletal proteins are the structural support of dendrites and axons. We have previously shown, in the mouse cerebral cortex and now with this work in spinal cord, that rabies virus generates remarkable alterations in the morphological pattern of the neurons and that this effect is associated with the increase in the expression of two cytoskeletal proteins (MAP2 and NF-H). It is necessary to deepen the investigation of the pathogenesis of rabies in order to find therapeutic alternatives to a disease to which the World Health Organization classifies as a neglected disease.<\/jats:p>","DOI":"10.3390\/v10030112","type":"journal-article","created":{"date-parts":[[2018,3,6]],"date-time":"2018-03-06T12:16:27Z","timestamp":1520338587000},"page":"112","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Overexpression of MAP2 and NF-H Associated with Dendritic Pathology in the Spinal Cord of Mice Infected with Rabies Virus"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9765-0461","authenticated-orcid":false,"given":"Jeison","family":"Monroy-G\u00f3mez","sequence":"first","affiliation":[{"name":"Grupo de Morfolog\u00eda Celular, Instituto Nacional de Salud (INS), Bogot\u00e1 111321, D.C., Colombia"},{"name":"Departamento de Ciencias B\u00e1sicas, Instituci\u00f3n Universitaria Escuela Colombiana de Rehabilitaci\u00f3n, Bogot\u00e1 110121, D.C., Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerardo","family":"Santamar\u00eda","sequence":"additional","affiliation":[{"name":"Grupo de Morfolog\u00eda Celular, Instituto Nacional de Salud (INS), Bogot\u00e1 111321, D.C., Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Orlando","family":"Torres-Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Grupo de Morfolog\u00eda Celular, Instituto Nacional de Salud (INS), Bogot\u00e1 111321, D.C., Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,3,6]]},"reference":[{"key":"ref_1","unstructured":"Brady, S., Siegel, G., Weyne, A., and Price, D. (2012). The cytoskeleton of neuron and glia. Basic Neurochemistry: Principles of Molecular, Cellular and Medical Neurobiology, Academic Press. [8th ed.]."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1590\/S1676-26492011000100005","article-title":"O papel das prote\u00ednas do citoesqueleto na fisiologia celular normal e em condi\u00e7\u00f5es patol\u00f3gicas","volume":"17","author":"Monteiro","year":"2011","journal-title":"J. Epilepsy Clin. Neurophysiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/gb-2004-6-1-204","article-title":"The MAP2\/Tau family of microtubule-associated proteins","volume":"6","author":"Dehmelt","year":"2004","journal-title":"Genome Biol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"3057","DOI":"10.1007\/s00018-004-4268-8","article-title":"Neurofilament proteins in neurodegenerative diseases","volume":"61","author":"Liua","year":"2004","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"3257","DOI":"10.1242\/jcs.104729","article-title":"Neurofilaments at a glance","volume":"125","author":"Yuan","year":"2012","journal-title":"J. Cell Sci."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Jackson, A. (2013). Pathology. Rabies, Academic Press.","DOI":"10.1007\/978-3-0348-0425-7_9"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1080\/13550280802216502","article-title":"Neuronal apoptosis does not play an important role in human rabies encephalitis","volume":"14","author":"Jackson","year":"2008","journal-title":"J. Neurovirol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Suja, M., Mahadevan, A., Madhusudana, S., and Shankar, S. (2011). Role of apoptosis in rabies viral encephalitis: A comparative study in mice, canine, and human brain with a review of literature. Pathol. Res. Int., 1\u201313.","DOI":"10.4061\/2011\/374286"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1016\/S0065-3527(08)60090-1","article-title":"Pathophysiology of rabies virus infection of the nervous system","volume":"42","author":"Tsiang","year":"1993","journal-title":"Adv. Virus Res."},{"key":"ref_10","first-page":"239","article-title":"Modulation of the immune response in the nervous system by rabies virus","volume":"289","author":"Lafon","year":"2005","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"10063","DOI":"10.1128\/JVI.79.15.10063-10068.2005","article-title":"Degeneration of neuronal processes after infection with pathogenic, but not attenuated, rabies viruses","volume":"79","author":"Li","year":"2005","journal-title":"J. Virol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"465","DOI":"10.4067\/S0717-95022015000200010","article-title":"Immunohistochemical overexpression of MAP-2 in the cerebral cortex of rabies-infected mice","volume":"33","author":"Hurtado","year":"2015","journal-title":"Int. J. Morphol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1099\/vir.0.047480-0","article-title":"Street rabies virus causes dendritic injury and F-actin depolymerization in the hippocampus","volume":"94","author":"Song","year":"2013","journal-title":"J. Gen. Virol."},{"key":"ref_14","first-page":"96","article-title":"Patolog\u00eda dendr\u00edtica en rabia: Estudio neurohistol\u00f3gico, inmunohistoqu\u00edmico y ultraestructural en ratones","volume":"26","author":"Rengifo","year":"2014","journal-title":"Rev. Asoc. Colomb. Cienc. Biol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"605","DOI":"10.7705\/biomedica.v27i4.177","article-title":"Alteraciones de la morfolog\u00eda dendr\u00edtica neuronal en la corteza cerebral de ratones","volume":"27","author":"Yepes","year":"2007","journal-title":"Biom\u00e9dica"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1002\/jnr.490330402","article-title":"The role of microtubule-associated protein 2 (MAP-2) in neuronal growth, plasticity, and degeneration","volume":"33","author":"Johnson","year":"1992","journal-title":"J. Neurosci. Res."},{"key":"ref_17","first-page":"564","article-title":"Distribuci\u00f3n de calbindina y parvoalb\u00famina y efecto del virus de la rabia sobre su expresi\u00f3n en la m\u00e9dula espinal de ratones","volume":"33","year":"2013","journal-title":"Biom\u00e9dica"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1292\/jvms.09-0550","article-title":"Lesions of the central nervous system induced by intracerebral inoculation of BALB\/c mice with rabies virus (CVS-11)","volume":"72","author":"Kojima","year":"2010","journal-title":"J. Vet. Med. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1292\/jvms.71.319","article-title":"Pathology of the spinal cord of C57BL\/6J mice infected with rabies virus (CVS-11 Strain)","volume":"71","author":"Kojima","year":"2009","journal-title":"J. Vet. Med. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"146","DOI":"10.7705\/biomedica.v30i1.162","article-title":"Elaboraci\u00f3n y evaluaci\u00f3n de un antisuero para la detecci\u00f3n inmunohistoqu\u00edmica del virus de la rabia en tejido cerebral fijado en aldeh\u00eddos","volume":"30","author":"Lamprea","year":"2010","journal-title":"Biom\u00e9dica"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0165-0270(97)00163-5","article-title":"A method for vibratome sectioning of Golgi-Cox stained whole rat brain","volume":"79","author":"Gibb","year":"1998","journal-title":"J. Neurosci. Methods"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1559-0275-10-3","article-title":"Quantitative proteomics for identifying biomarkers for rabies","volume":"10","author":"Venugopal","year":"2013","journal-title":"Clin. Proteom."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"63","DOI":"10.7705\/biomedica.v24i1.1250","article-title":"Efecto de la infecci\u00f3n por el virus de la rabia sobre la expresi\u00f3n de parvoalb\u00famina, calbindina y calretinina en la corteza cerebral de ratones","volume":"24","author":"Yepes","year":"2004","journal-title":"Biom\u00e9dica"},{"key":"ref_24","first-page":"80","article-title":"Cambios en los sistemas de neurotransmisi\u00f3n excitador e inhibitorio en el cerebelo de ratones infectados con virus de la rabia","volume":"33","author":"Rengifo","year":"2013","journal-title":"Biom\u00e9dica"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2758","DOI":"10.1073\/pnas.051630298","article-title":"Effect of rabies virus infection on gene expression in mouse brain","volume":"98","author":"Prosniak","year":"2001","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_26","first-page":"749","article-title":"Comparative analysis of the pathogenic mechanisms of street rabies virus strains with different virulence levels","volume":"27","author":"Yin","year":"2014","journal-title":"Biomed. Environ. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Zan, J., Liu, S., Sun, D., Mo, K., Yan, Y., Liu, J., Hu, B.L., Gu, J.Y., Liao, M., and Zhou, J.Y. (2017). Rabies virus infection induces microtubule depolymerization to facilitate viral RNA synthesis by upregulating HDAC6. Front. Cell Infect. Microbiol., 7.","DOI":"10.3389\/fcimb.2017.00146"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2733","DOI":"10.1128\/jvi.61.9.2733-2741.1987","article-title":"Rabies virus infection of cultured rat sensory neurons","volume":"61","author":"Lycke","year":"1987","journal-title":"J. Virol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"14172","DOI":"10.1128\/JVI.02254-14","article-title":"Anterograde glycoprotein-dependent transport of newly generated rabies virus in dorsal root ganglion neurons","volume":"88","author":"Bauer","year":"2014","journal-title":"J. Virol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"766","DOI":"10.1016\/j.neuron.2013.12.033","article-title":"Mapping sensory circuits by anterograde trans-synaptic transfer of recombinant rabies virus","volume":"81","author":"Zampieri","year":"2014","journal-title":"Neuron"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"7229","DOI":"10.1073\/pnas.0701397104","article-title":"The dynein light chain 8 binding motif of rabies virus phosphoprotein promotes efficient viral transcription","volume":"104","author":"Tan","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Gluska, S., Zahavi, E., Chein, M., Gradus, T., Bauer, A., Finke, S., and Perlson, E. (2014). Rabies virus hijacks and accelerates the p75NTR retrograde axonal transport machinery. PLoS Pathog., 10.","DOI":"10.1371\/journal.ppat.1004348"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"10212","DOI":"10.1128\/JVI.74.21.10212-10216.2000","article-title":"Interaction of the rabies virus P protein with the LC8 dynein light chain","volume":"74","author":"Raux","year":"2000","journal-title":"J. Virol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"9591","DOI":"10.1128\/JVI.01298-15","article-title":"A dynein light chain 1 binding motif in rabies virus polymerase L protein plays a role in microtubule reorganization and viral primary transcription","volume":"88","author":"Bauer","year":"2015","journal-title":"J. Virol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"40793","DOI":"10.1074\/jbc.M112.394353","article-title":"Dynein light chain 1 (LC8) association enhances microtubule stability and promotes microtubule bundling","volume":"287","author":"Asthana","year":"2012","journal-title":"J. Biol. Chem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1016\/j.chom.2013.10.012","article-title":"HIV-1 induces the formation of stable microtubules to enhance early infection","volume":"14","author":"Sabo","year":"2013","journal-title":"Cell Host Microbe"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"992","DOI":"10.1093\/cercor\/10.10.992","article-title":"Dendritic cytoskeletal protein expression in mental retardation: An immunohistochemical study of the neocortex in Rett syndrome","volume":"10","author":"Kaufmann","year":"2000","journal-title":"Cereb. Cortex"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1083\/jcb.135.3.711","article-title":"Neurofilament subunit NF-H modulates axonal diameter by selectively slowing neurofilament transport","volume":"135","author":"Marszalek","year":"1996","journal-title":"J. Cell Biol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1002\/jnr.10212","article-title":"Overexpression of neurofilament H disrupts normal cell structure and function","volume":"68","author":"Szebenyi","year":"2002","journal-title":"J. Neurosci. Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.1083\/jcb.140.5.1167","article-title":"Antagonistic roles of neurofilament subunits NF-H and NF-M against NF-L in shaping dendritic arborization in spinal motor neurons","volume":"140","author":"Kong","year":"1998","journal-title":"J. Cell Biol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"9","DOI":"10.17151\/biosa.2016.15.1.2","article-title":"Ultraestructura dendr\u00edtica en neuronas piramidales de ratones inoculados con virus de la rabia","volume":"15","author":"Sarmiento","year":"2016","journal-title":"Biosalud"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1413","DOI":"10.1083\/jcb.130.6.1413","article-title":"Increasing neurofilament subunit NF-M expression reduces axonal NF-H, inhibits radial growth, and results in neurofilamentous accumulation in motor neurons","volume":"130","author":"Wong","year":"1995","journal-title":"J. Cell Biol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1159\/000098464","article-title":"Dementia and art: Neuronal intermediate filament inclusion disease and dissolution of artistic creativity","volume":"57","author":"Budrys","year":"2007","journal-title":"Eur. Neurol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1002\/ana.24980","article-title":"Phosphorylated neurofilament heavy chain: A biomarker of survival for C9ORF72-associated amyotrophic lateral sclerosis","volume":"82","author":"Gendron","year":"2017","journal-title":"Ann. Neurol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1945","DOI":"10.1212\/WNL.0000000000001015","article-title":"CSF neurofilament light differs in neurodegenerative diseases and predicts severity and survival","volume":"83","author":"Farahmand","year":"2014","journal-title":"Neurology"}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/10\/3\/112\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T14:57:38Z","timestamp":1760194658000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/10\/3\/112"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,3,6]]},"references-count":45,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2018,3]]}},"alternative-id":["v10030112"],"URL":"https:\/\/doi.org\/10.3390\/v10030112","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,3,6]]}}}