{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T17:48:34Z","timestamp":1792000114786,"version":"4.4.0"},"reference-count":36,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2018,3,27]],"date-time":"2018-03-27T00:00:00Z","timestamp":1522108800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Bundesministerium f\u00fcr Gesundheit; Germany"},{"name":"LOEWE Center DRUID"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>The Zika virus (ZIKV) outbreak in 2016 in South America with specific pathogenic outcomes highlighted the need for new antiviral substances with broad-spectrum activities to react quickly to unexpected outbreaks of emerging viral pathogens. Very recently, the natural compound silvestrol isolated from the plant Aglaia foveolata was found to have very potent antiviral effects against the (\u2212)-strand RNA-virus Ebola virus as well as against Corona- and Picornaviruses with a (+)-strand RNA-genome. This antiviral activity is based on the impaired translation of viral RNA by the inhibition of the DEAD-box RNA helicase eukaryotic initiation factor-4A (eIF4A) which is required to unwind structured 5\u00b4-untranslated regions (5\u2032-UTRs) of several proto-oncogenes and thereby facilitate their translation. Zika virus is a flavivirus with a positive-stranded RNA-genome harboring a 5\u2032-capped UTR with distinct secondary structure elements. Therefore, we investigated the effects of silvestrol on ZIKV replication in A549 cells and primary human hepatocytes. Two different ZIKV strains were used. In both infected A549 cells and primary human hepatocytes, silvestrol has the potential to exert a significant inhibition of ZIKV replication for both analyzed strains, even though the ancestor strain from Uganda is less sensitive to silvestrol. Our data might contribute to identify host factors involved in the control of ZIKV infection and help to develop antiviral concepts that can be used to treat a variety of viral infections without the risk of resistances because a host protein is targeted.<\/jats:p>","DOI":"10.3390\/v10040149","type":"journal-article","created":{"date-parts":[[2018,3,27]],"date-time":"2018-03-27T12:17:24Z","timestamp":1522153044000},"page":"149","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":72,"title":["Inhibition of Zika Virus Replication by Silvestrol"],"prefix":"10.3390","volume":"10","author":[{"given":"Fabian","family":"Elgner","sequence":"first","affiliation":[{"name":"Department of Virology, Paul-Ehrlich-Institut, 63225 Langen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0586-1235","authenticated-orcid":false,"given":"Catarina","family":"Sabino","sequence":"additional","affiliation":[{"name":"Department of Virology, Paul-Ehrlich-Institut, 63225 Langen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Basic","sequence":"additional","affiliation":[{"name":"Department of Virology, Paul-Ehrlich-Institut, 63225 Langen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Ploen","sequence":"additional","affiliation":[{"name":"Department of Virology, Paul-Ehrlich-Institut, 63225 Langen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnold","family":"Gr\u00fcnweller","sequence":"additional","affiliation":[{"name":"Pharmazeutische Chemie, Philipps-Universit\u00e4t Marburg, 35037 Marburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eberhard","family":"Hildt","sequence":"additional","affiliation":[{"name":"Department of Virology, Paul-Ehrlich-Institut, 63225 Langen, Germany"},{"name":"German Center for Infection Research (DZIF), 38124 Braunschweig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,3,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"G\u00f6ertz, G.P., Abbo, S.R., Fros, J.J., and Pijlman, G.P. 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