{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T16:55:16Z","timestamp":1790787316430,"version":"4.1.0"},"reference-count":110,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2018,4,16]],"date-time":"2018-04-16T00:00:00Z","timestamp":1523836800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Arthropod-borne viruses (arboviruses) are resurging across the globe. Zika virus (ZIKV) has caused significant concern in recent years because it can lead to congenital malformations in babies and Guillain-Barr\u00e9 syndrome in adults. Unlike other arboviruses, ZIKV can be sexually transmitted and may persist in the male reproductive tract. There is limited information regarding the impact of ZIKV on male reproductive health and fertility. Understanding the mechanisms that underlie persistent ZIKV infections in men is critical to developing effective vaccines and therapies. Mouse and macaque models have begun to unravel the pathogenesis of ZIKV infection in the male reproductive tract, with the testes and prostate gland implicated as potential reservoirs for persistent ZIKV infection. Here, we summarize current knowledge regarding the pathogenesis of ZIKV in the male reproductive tract, the development of animal models to study ZIKV infection at this site, and prospects for vaccines and therapeutics against persistent ZIKV infection.<\/jats:p>","DOI":"10.3390\/v10040198","type":"journal-article","created":{"date-parts":[[2018,4,16]],"date-time":"2018-04-16T12:40:26Z","timestamp":1523882426000},"page":"198","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":58,"title":["Zika Virus in the Male Reproductive Tract"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9320-3963","authenticated-orcid":false,"given":"Liesel","family":"Stassen","sequence":"first","affiliation":[{"name":"Institute of Health and Biomedical Innovation, and School of Biomedical Sciences, Queensland University of Technology, Brisbane 4006, Queensland, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles W.","family":"Armitage","sequence":"additional","affiliation":[{"name":"Institute of Health and Biomedical Innovation, and School of Biomedical Sciences, Queensland University of Technology, Brisbane 4006, Queensland, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David J.","family":"Van der Heide","sequence":"additional","affiliation":[{"name":"Institute of Health and Biomedical Innovation, and School of Biomedical Sciences, Queensland University of Technology, Brisbane 4006, Queensland, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth W.","family":"Beagley","sequence":"additional","affiliation":[{"name":"Institute of Health and Biomedical Innovation, and School of Biomedical Sciences, Queensland University of Technology, Brisbane 4006, Queensland, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8628-4216","authenticated-orcid":false,"given":"Francesca D.","family":"Frentiu","sequence":"additional","affiliation":[{"name":"Institute of Health and Biomedical Innovation, and School of Biomedical Sciences, Queensland University of Technology, Brisbane 4006, Queensland, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,4,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1056\/NEJMoa1600651","article-title":"Zika virus associated with microcephaly","volume":"374","author":"Mlakar","year":"2016","journal-title":"N. Engl. J. Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e13","DOI":"10.1038\/emi.2016.141","article-title":"Zika virus genome biology and molecular pathogenesis","volume":"6","author":"Wang","year":"2017","journal-title":"Emerg. Microbes Infect."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1007\/s12275-017-7063-6","article-title":"Zika virus: An emerging flavivirus","volume":"55","author":"Yun","year":"2017","journal-title":"J. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Holbrook, M.R. (2017). Historical perspectives on Flavivirus research. Viruses, 9.","DOI":"10.3390\/v9050097"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/0035-9203(58)90085-3","article-title":"Zika virus: Further isolations in the Zika area, and some studies on the strains isolated","volume":"52","author":"Weinbren","year":"1958","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1016\/j.bbrc.2017.05.050","article-title":"Interaction of Flavivirus with their mosquito vectors and their impact on the human health in the Americas","volume":"492","author":"Valderrama","year":"2017","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/0035-9203(52)90042-4","article-title":"Zika virus. 1. Isolations and serological specificity","volume":"46","author":"Dick","year":"1952","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"223","DOI":"10.4049\/jimmunol.69.2.223","article-title":"Neutralizing antibodies against certain recently isolated viruses in the sera of human beings residing in East Africa","volume":"69","author":"Smithburn","year":"1952","journal-title":"J. Immunol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"860","DOI":"10.1128\/JCM.00279-16","article-title":"Zika virus: Diagnostics for an emerging pandemic threat","volume":"54","author":"Waggoner","year":"2016","journal-title":"J. Clin. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"675","DOI":"10.2471\/BLT.16.171082","article-title":"Zika: The origin and spread of a mosquito-borne virus","volume":"94","author":"Kindhauser","year":"2016","journal-title":"Bull. World Health Organ."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2536","DOI":"10.1056\/NEJMoa0805715","article-title":"Zika virus outbreak on Yap Island, Federated States of Micronesia","volume":"360","author":"Duffy","year":"2009","journal-title":"N. Engl. J. Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1016\/j.jinf.2016.02.011","article-title":"Zika fever and congenital Zika syndrome: An unexpected emerging arboviral disease","volume":"72","author":"Chan","year":"2016","journal-title":"J. Infect."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1232","DOI":"10.3201\/eid1408.080287","article-title":"Genetic and serologic properties of Zika virus associated with an epidemic, Yap State, Micronesia, 2007","volume":"14","author":"Lanciotti","year":"2008","journal-title":"Emerg. Infect. Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"669","DOI":"10.3201\/eid2304.161549","article-title":"Zika virus seroprevalence, French Polynesia, 2014\u20132015","volume":"23","author":"Aubry","year":"2017","journal-title":"Emerg. Infect. Dis."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/j.medmal.2014.04.008","article-title":"Current Zika virus epidemiology and recent epidemics","volume":"44","author":"Ioos","year":"2014","journal-title":"Med. Mal. Infect."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"O595","DOI":"10.1111\/1469-0691.12707","article-title":"Rapid spread of emerging Zika virus in the Pacific area","volume":"20","author":"Musso","year":"2014","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2125","DOI":"10.1016\/S0140-6736(16)00651-6","article-title":"Association between Zika virus and microcephaly in French Polynesia, 2013-15: A retrospective study","volume":"387","author":"Cauchemez","year":"2016","journal-title":"Lancet"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e3257","DOI":"10.1097\/MD.0000000000003257","article-title":"Guillain-Barre syndrome (42 cases) occurring during a Zika virus outbreak in French Polynesia","volume":"95","author":"Watrin","year":"2016","journal-title":"Medicine"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"55","DOI":"10.15585\/mmwr.mm6503e1","article-title":"Zika virus spreads to new areas\u2014Region of the Americas, May 2015\u2013January 2016","volume":"65","author":"Hennessey","year":"2016","journal-title":"MMWR Morb. Mortal. Wkly. Rep."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1126\/science.aaf5036","article-title":"Zika virus in the Americas: Early epidemiological and genetic findings","volume":"352","author":"Faria","year":"2016","journal-title":"Science"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2321","DOI":"10.1056\/NEJMoa1602412","article-title":"Zika virus infection in pregnant women in Rio de Janeiro","volume":"375","author":"Brasil","year":"2016","journal-title":"N. Engl. J. Med."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1002\/uog.17404","article-title":"Association between Zika virus and fetopathy: A prospective cohort study in French Guiana","volume":"49","author":"Pomar","year":"2017","journal-title":"Ultrasound Obstet. Gynecol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"405","DOI":"10.3201\/eid2303.161499","article-title":"Zika virus RNA replication and persistence in brain and placental tissue","volume":"23","author":"Bhatnagar","year":"2017","journal-title":"Emerg. Infect. Dis."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1531","DOI":"10.1016\/S0140-6736(16)00562-6","article-title":"Guillain-Barre syndrome outbreak associated with Zika virus infection in French Polynesia: A case-control study","volume":"387","author":"Blake","year":"2016","journal-title":"Lancet"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1016\/S0140-6736(16)00339-1","article-title":"Guillain-Barre syndrome","volume":"388","author":"Willison","year":"2016","journal-title":"Lancet"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.jcv.2016.08.297","article-title":"Fatal encephalitis associated with Zika virus infection in an adult","volume":"83","author":"Soares","year":"2016","journal-title":"J. Clin. Virol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"696","DOI":"10.3201\/eid2304.161967","article-title":"Severe thrombocytopenia after Zika virus infection, Guadeloupe, 2016","volume":"23","author":"Chammard","year":"2017","journal-title":"Emerg. Infect. Dis."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1093\/cid\/ciw770","article-title":"Transient hearing loss in adults associated with Zika virus infection","volume":"64","author":"Vinhaes","year":"2017","journal-title":"Clin. Infect. Dis."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"880","DOI":"10.3201\/eid1705.101939","article-title":"Probable non-vector-borne transmission of Zika virus, Colorado, USA","volume":"17","author":"Foy","year":"2011","journal-title":"Emerg. Infect. Dis."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2195","DOI":"10.1056\/NEJMc1604449","article-title":"Evidence of sexual transmission of Zika virus","volume":"374","author":"Matheron","year":"2016","journal-title":"N. Engl. J. Med."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"McDonald, E.M., Duggal, N.K., and Brault, A.C. (2017). Pathogenesis and sexual transmission of Spondweni and Zika viruses. PLoS Negl. Trop. Dis., 11.","DOI":"10.1371\/journal.pntd.0005990"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"372","DOI":"10.15585\/mmwr.mm6514a3","article-title":"Male-to-male sexual transmission of Zika virus\u2014Texas, January 2016","volume":"65","author":"Deckard","year":"2016","journal-title":"MMWR Morb. Mortal. Wkly. Rep."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"716","DOI":"10.15585\/mmwr.mm6528e2","article-title":"Suspected female-to-male sexual transmission of Zika virus\u2014New York City, 2016","volume":"65","author":"Davidson","year":"2016","journal-title":"MMWR Morb. Mortal. Wkly. Rep."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"359","DOI":"10.3201\/eid2102.141363","article-title":"Potential sexual transmission of Zika virus","volume":"21","author":"Musso","year":"2015","journal-title":"Emerg. Infect. Dis."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2501","DOI":"10.1016\/S0140-6736(16)30775-9","article-title":"Late sexual transmission of Zika virus related to persistence in the semen","volume":"387","author":"Turmel","year":"2016","journal-title":"Lancet"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.ijid.2016.08.023","article-title":"Higher incidence of Zika in adult women than adult men in Rio de Janeiro suggests a significant contribution of sexual transmission from men to women","volume":"51","author":"Coelho","year":"2016","journal-title":"Int. J. Infect. Dis."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"915","DOI":"10.15585\/mmwr.mm6534e2","article-title":"Likely sexual transmission of Zika virus from a man with no symptoms of infection\u2014Maryland, 2016","volume":"65","author":"Brooks","year":"2016","journal-title":"MMWR Morb. Mortal. Wkly. Rep."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"10","DOI":"10.2807\/1560-7917.ES.2016.21.23.30254","article-title":"Sexual transmission of Zika virus in an entirely asymptomatic couple returning from a Zika epidemic area, France, April 2016","volume":"21","author":"Freour","year":"2016","journal-title":"Euro Surveill."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.epidem.2017.06.003","article-title":"Zika virus dynamics: When does sexual transmission matter?","volume":"21","author":"Maxian","year":"2017","journal-title":"Epidemics"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"28070","DOI":"10.1038\/srep28070","article-title":"Prevention and control of Zika as a mosquito-borne and sexually transmitted disease: A mathematical modeling analysis","volume":"6","author":"Gao","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Allard, A., Althouse, B.M., Hebert-Dufresne, L., and Scarpino, S.V. (2017). The risk of sustained sexual transmission of Zika is underestimated. PLoS Pathog., 13.","DOI":"10.1371\/journal.ppat.1006633"},{"key":"ref_42","unstructured":"Pacheco, O., Beltr\u00e1n, M., Nelson, C.A., Valencia, D., Tolosa, N., Farr, S.L., Padilla, A.V., Tong, V.T., Cuevas, E.L., and Espinosa-Bode, A. (2016). Zika virus disease in Colombia\u2014Preliminary report. N. Engl. J. Med."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1100","DOI":"10.1016\/S1473-3099(16)30324-3","article-title":"Low risk of a sexually-transmitted Zika virus outbreak","volume":"16","author":"Yakob","year":"2016","journal-title":"Lancet Infect. Dis."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1274","DOI":"10.3201\/eid2308.170036","article-title":"High infection rates for adult macaques after intravaginal or intrarectal inoculation with Zika virus","volume":"23","author":"Haddow","year":"2017","journal-title":"Emerg. Infect. Dis."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"894","DOI":"10.1016\/S1473-3099(16)30153-0","article-title":"Zika virus in semen of a patient returning from a non-epidemic area","volume":"16","author":"Mansuy","year":"2016","journal-title":"Lancet Infect. Dis."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1264","DOI":"10.1093\/cid\/ciw509","article-title":"Long-lasting persistence of Zika virus in semen","volume":"63","author":"Matheron","year":"2016","journal-title":"Clin. Infect. Dis."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"6","DOI":"10.2807\/1560-7917.ES.2016.21.32.30314","article-title":"Persistent detection of Zika virus RNA in semen for six months after symptom onset in a traveller returning from Haiti to Italy, February 2016","volume":"21","author":"Nicastri","year":"2016","journal-title":"Euro Surveill."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1200","DOI":"10.1016\/S1473-3099(17)30444-9","article-title":"Effect of acute Zika virus infection on sperm and virus clearance in body fluids: A prospective observational study","volume":"17","author":"Joguet","year":"2017","journal-title":"Lancet Infect. Dis."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1106","DOI":"10.1016\/S1473-3099(16)30336-X","article-title":"Zika virus in semen and spermatozoa","volume":"16","author":"Mansuy","year":"2016","journal-title":"Lancet Infect. Dis."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1001.e1","DOI":"10.1016\/j.cmi.2017.07.006","article-title":"Detection of ZIKV RNA in semen of asymptomatic blood donors","volume":"23","author":"Musso","year":"2017","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.jcv.2017.10.006","article-title":"Persistence and infectivity of Zika virus in semen after returning from endemic areas: Report of 5 cases","volume":"96","author":"Arribas","year":"2017","journal-title":"J. Clin. Virol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1720","DOI":"10.1093\/infdis\/jix204","article-title":"Fetal growth restriction caused by sexual transmission of Zika virus in mice","volume":"215","author":"Uraki","year":"2017","journal-title":"J. Infect. Dis."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1097\/PAI.0000000000000580","article-title":"Cellular targets and receptor of sexual transmission of Zika virus","volume":"25","author":"Bagasra","year":"2017","journal-title":"Appl. Immunohistochem. Mol. Morphol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"611","DOI":"10.3201\/eid2304.161692","article-title":"Presence and persistence of Zika virus RNA in semen, United Kingdom, 2016","volume":"23","author":"Atkinson","year":"2017","journal-title":"Emerg. Infect. Dis."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1220","DOI":"10.1016\/S1473-3099(16)30387-5","article-title":"Zika virus in the female genital tract","volume":"16","author":"Visseaux","year":"2016","journal-title":"Lancet Infect. Dis."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"557","DOI":"10.2174\/1381612043453225","article-title":"Viruses in semen and male genital tissues\u2014Consequences for the reproductive system and therapeutic perspectives","volume":"10","author":"Jegou","year":"2004","journal-title":"Curr. Pharm. Des."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1111\/j.1600-065X.2006.00438.x","article-title":"The testis in immune privilege","volume":"213","author":"Fijak","year":"2006","journal-title":"Immunol. Rev."},{"key":"ref_58","first-page":"1195","article-title":"Immunophysiology of the male reproductive tract","volume":"Volume 1, 2","author":"Neil","year":"2006","journal-title":"Knobil and Neill\u2019s Physiology of Reproduction"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1210\/er.2014-1101","article-title":"The mammalian blood-testis barrier: Its biology and regulation","volume":"36","author":"Mruk","year":"2015","journal-title":"Endocr. Rev."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/S0165-0378(02)00016-5","article-title":"Macrophages and the immune responsiveness of the testis","volume":"57","author":"Hedger","year":"2002","journal-title":"J. Reprod. Immunol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.ijid.2016.08.025","article-title":"Microhematospermia in acute Zika virus infection","volume":"51","author":"Torres","year":"2016","journal-title":"Int. J. Infect. Dis."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Huits, R.M.H.G., De Smet, B., Ari\u00ebn, K.K., Van Esbroeck, M., de Jong, B.C., Bottieau, E., and Cnops, L. (2016). Kinetics of Zika virus persistence in semen. Bull. World Health Organ.","DOI":"10.2471\/BLT.16.181370"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"e00623-17","DOI":"10.1128\/JVI.00623-17","article-title":"Zika virus infects human Sertoli cells and modulates the integrity of the in vitro blood-testis barrier model","volume":"91","author":"Siemann","year":"2017","journal-title":"J. Virol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1056\/NEJMc1612600","article-title":"Kinetics of Zika viral load in semen","volume":"377","author":"Matheus","year":"2017","journal-title":"N. Engl. J. Med."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"8880","DOI":"10.1128\/JVI.00354-15","article-title":"Biology of Zika virus infection in human skin cells","volume":"89","author":"Hamel","year":"2015","journal-title":"J. Virol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"14408","DOI":"10.1073\/pnas.1618029113","article-title":"Zika virus cell tropism in the developing human brain and inhibition by azithromycin","volume":"113","author":"Retallack","year":"2016","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"2024","DOI":"10.1073\/pnas.1620558114","article-title":"AXL-dependent infection of human fetal endothelial cells distinguishes Zika virus from other pathogenic flaviviruses","volume":"114","author":"Richard","year":"2017","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"5477","DOI":"10.1038\/s41598-018-23899-x","article-title":"Human Sertoli cells support high levels of Zika virus replication and persistence","volume":"8","author":"Kumar","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1355","DOI":"10.1369\/jhc.5A6637.2005","article-title":"Immunoexpression of Tyro 3 family receptors\u2014Tyro 3, Axl, and Mer\u2014And their ligand Gas6 in postnatal developing mouse testis","volume":"53","author":"Wang","year":"2005","journal-title":"J. Histochem. Cytochem."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"10109","DOI":"10.1128\/JVI.01157-06","article-title":"Tyro3 family-mediated cell entry of Ebola and Marburg viruses","volume":"80","author":"Shimojima","year":"2006","journal-title":"J. Virol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1922","DOI":"10.3201\/eid2311.171049","article-title":"The breadth of viruses in human semen","volume":"23","author":"Salam","year":"2017","journal-title":"Emerg. Infect. Dis."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1016\/S1473-3099(16)30320-6","article-title":"Probable sexual transmission of Zika virus from a vasectomised man","volume":"16","author":"Arsuaga","year":"2016","journal-title":"Lancet Infect. Dis."},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Froeschl, G., Huber, K., von Sonnenburg, F., Nothdurft, H.D., Bretzel, G., Hoelscher, M., Zoeller, L., Trottmann, M., Pan-Montojo, F., and Dobler, G. (2017). Long-term kinetics of Zika virus RNA and antibodies in body fluids of a vasectomized traveller returning from Martinique: A case report. BMC Infect. Dis., 17.","DOI":"10.1186\/s12879-016-2123-9"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1093\/infdis\/jix436","article-title":"Replication of Zika virus in human prostate cells: A potential source of sexually transmitted virus","volume":"217","author":"Spencer","year":"2017","journal-title":"J. Infect. Dis."},{"key":"ref_75","first-page":"41","article-title":"Ejaculatory physiology and pathophysiology: Assessment and treatment in male infertility","volume":"3","author":"Revenig","year":"2014","journal-title":"Transl. Androl. Urol."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1038\/nature20556","article-title":"Zika virus infection damages the testes in mice","volume":"540","author":"Govero","year":"2016","journal-title":"Nature"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"1511.e10","DOI":"10.1016\/j.cell.2016.11.016","article-title":"Zika virus causes testis damage and leads to male infertility in mice","volume":"167","author":"Ma","year":"2016","journal-title":"Cell"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"e1602899","DOI":"10.1126\/sciadv.1602899","article-title":"Zika virus causes testicular atrophy","volume":"3","author":"Uraki","year":"2017","journal-title":"Sci. Adv."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"272","DOI":"10.3389\/fcimb.2017.00272","article-title":"Sertoli cells are susceptible to ZIKV infection in mouse testis","volume":"7","author":"Sheng","year":"2017","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1186\/s12985-017-0749-x","article-title":"Tissue tropisms, infection kinetics, histologic lesions, and antibody response of the MR766 strain of Zika virus in a murine model","volume":"14","author":"Kawiecki","year":"2017","journal-title":"Virol. J."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/s41467-017-00737-8","article-title":"A single-dose live-attenuated vaccine prevents Zika virus pregnancy transmission and testis damage","volume":"8","author":"Shan","year":"2017","journal-title":"Nat. Commun."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.antiviral.2017.09.005","article-title":"Human polyclonal antibodies produced in transchromosomal cattle prevent lethal Zika virus infection and testicular atrophy in mice","volume":"146","author":"Stein","year":"2017","journal-title":"Antivir. Res."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"3526","DOI":"10.4049\/jimmunol.1601949","article-title":"Adaptive immune responses to Zika virus are important for controlling virus infection and preventing infection in brain and testes","volume":"198","author":"Winkler","year":"2017","journal-title":"J. Immunol."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"1751","DOI":"10.1016\/j.celrep.2017.01.056","article-title":"Frequent Zika virus sexual transmission and prolonged viral RNA shedding in an immunodeficient mouse model","volume":"18","author":"Duggal","year":"2017","journal-title":"Cell Rep."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"720","DOI":"10.1016\/j.chom.2016.03.010","article-title":"A mouse model of Zika virus pathogenesis","volume":"19","author":"Lazear","year":"2016","journal-title":"Cell Host Microbe"},{"key":"ref_86","doi-asserted-by":"crossref","unstructured":"Dowall, S.D., Graham, V.A., Rayner, E., Hunter, L., Atkinson, B., Pearson, G., Dennis, M., and Hewson, R. (2017). Lineage-dependent differences in the disease progression of Zika virus infection in type-I interferon receptor knockout (A129) mice. PLoS Negl. Trop. Dis., 11.","DOI":"10.1371\/journal.pntd.0005704"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"15743","DOI":"10.1038\/ncomms15743","article-title":"DNA vaccination protects mice against Zika virus-induced damage to the testes","volume":"8","author":"Griffin","year":"2017","journal-title":"Nat. Commun."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/j.ebiom.2016.11.017","article-title":"Zika virus infection in dexamethasone-immunosuppressed mice demonstrating disseminated infection with multi-organ involvement including orchitis effectively treated by recombinant type I interferons","volume":"14","author":"Chan","year":"2016","journal-title":"EBioMedicine"},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"904","DOI":"10.1016\/j.ajpath.2017.12.019","article-title":"Comparative histopathologic lesions of the male reproductive tract during acute Infection of Zika virus in AG129 and IFNAR-\/- mice","volume":"188","author":"Clancy","year":"2018","journal-title":"Am. J. Pathol."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"1230","DOI":"10.1038\/s41467-018-03662-6","article-title":"A vaccinia-based single vector construct multi-pathogen vaccine protects against both Zika and chikungunya viruses","volume":"9","author":"Prow","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"4510","DOI":"10.1038\/s41598-018-22840-6","article-title":"Sexual transmission of Zika virus enhances in utero transmission in a mouse model","volume":"8","author":"Duggal","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.virol.2017.04.013","article-title":"Zika virus infection of adult and fetal STAT2 knock-out hamsters","volume":"507","author":"Siddharthan","year":"2017","journal-title":"Virology"},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"12204","DOI":"10.1038\/ncomms12204","article-title":"A rhesus macaque model of Asian-lineage Zika virus infection","volume":"7","author":"Dudley","year":"2016","journal-title":"Nat. Commun."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.ebiom.2016.09.022","article-title":"Characterization of a 2016 clinical isolate of Zika virus in non-human primates","volume":"12","author":"Li","year":"2016","journal-title":"EBioMedicine"},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"1448","DOI":"10.1038\/nm.4206","article-title":"Zika viral dynamics and shedding in rhesus and cynomolgus macaques","volume":"22","author":"Osuna","year":"2016","journal-title":"Nat. Med."},{"key":"ref_96","doi-asserted-by":"crossref","unstructured":"Nguyen, S.M., Antony, K.M., Dudley, D.M., Kohn, S., Simmons, H.A., Wolfe, B., Salamat, M.S., Teixeira, L.B.C., Wiepz, G.J., and Thoong, T.H. (2017). Highly efficient maternal-fetal Zika virus transmission in pregnant rhesus macaques. PLoS Pathog., 13.","DOI":"10.1371\/journal.ppat.1006378"},{"key":"ref_97","doi-asserted-by":"crossref","unstructured":"Coffey, L.L., Pesavento, P.A., Keesler, R.I., Singapuri, A., Watanabe, J., Watanabe, R., Yee, J., Bliss-Moreau, E., Cruzen, C., and Christe, K.L. (2017). Zika virus tissue and blood compartmentalization in acute infection of rhesus macaques. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0171148"},{"key":"ref_98","doi-asserted-by":"crossref","unstructured":"Hirsch, A.J., Smith, J.L., Haese, N.N., Broeckel, R.M., Parkins, C.J., Kreklywich, C., DeFilippis, V.R., Denton, M., Smith, P.P., and Messer, W.B. (2017). Zika virus infection of rhesus macaques leads to viral persistence in multiple tissues. PLoS Pathog., 13.","DOI":"10.1371\/journal.ppat.1006219"},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1126\/science.aah6157","article-title":"Protective efficacy of multiple vaccine platforms against Zika virus challenge in rhesus monkeys","volume":"353","author":"Abbink","year":"2016","journal-title":"Science"},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"2028","DOI":"10.3389\/fmicb.2016.02028","article-title":"Development of a Zika virus infection model in cynomolgus macaques","volume":"7","author":"Koide","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"1076","DOI":"10.1038\/mi.2015.125","article-title":"Induction of partial immunity in both males and females is sufficient to protect females against sexual transmission of Chlamydia","volume":"9","author":"Armitage","year":"2016","journal-title":"Mucosal Immunol."},{"key":"ref_102","doi-asserted-by":"crossref","unstructured":"Aliota, M.T., Dudley, D.M., Newman, C.M., Mohr, E.L., Gellerup, D.D., Breitbach, M.E., Buechler, C.R., Rasheed, M.N., Mohns, M.S., and Weiler, A.M. (2016). Heterologous protection against Asian Zika virus challenge in rhesus macaques. PLoS. Negl. Trop. Dis., 10.","DOI":"10.1101\/059592"},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.ejim.2017.08.001","article-title":"Current status of therapeutic and vaccine approaches against Zika virus","volume":"44","author":"Wahid","year":"2017","journal-title":"Eur. J. Intern. Med."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"2777","DOI":"10.1097\/QAD.0000000000001282","article-title":"Immune tolerance properties of the testicular tissue as a viral sanctuary site in ART-treated HIV-infected adults","volume":"30","author":"Jenabian","year":"2016","journal-title":"AIDS"},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.antiviral.2017.08.007","article-title":"Ribavirin inhibits Zika virus (ZIKV) replication in vitro and suppresses viremia in ZIKV-infected STAT1-deficient mice","volume":"146","author":"Kamiyama","year":"2017","journal-title":"Antivir. Res."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"1261","DOI":"10.1038\/ni.3849","article-title":"Human antibodies to the dengue virus E-dimer epitope have therapeutic activity against Zika virus infection","volume":"18","author":"Fernandez","year":"2017","journal-title":"Nat. Immunol."},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1038\/nature18952","article-title":"Vaccine protection against Zika virus from Brazil","volume":"536","author":"Larocca","year":"2016","journal-title":"Nature"},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1038\/nature21428","article-title":"Zika virus protection by a single low-dose nucleoside-modified mRNA vaccination","volume":"543","author":"Pardi","year":"2017","journal-title":"Nature"},{"key":"ref_109","unstructured":"Tebas, P., Roberts, C.C., Muthumani, K., Reuschel, E.L., Kudchodkar, S.B., Zaidi, F.I., White, S., Khan, A.S., Racine, T., and Choi, H. (2017). Safety and immunogenicity of an anti-Zika virus DNA vaccine\u2014Preliminary report. N. Engl. J. Med."},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1002\/pd.4880","article-title":"Imaging of congenital Zika virus infection: The route to identification of prognostic factors","volume":"36","author":"Vouga","year":"2016","journal-title":"Prenat. Diagn."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/10\/4\/198\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T15:00:54Z","timestamp":1760194854000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/10\/4\/198"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,4,16]]},"references-count":110,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2018,4]]}},"alternative-id":["v10040198"],"URL":"https:\/\/doi.org\/10.3390\/v10040198","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,4,16]]}}}