{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T09:09:40Z","timestamp":1766048980114,"version":"build-2065373602"},"reference-count":37,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2018,5,24]],"date-time":"2018-05-24T00:00:00Z","timestamp":1527120000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"UC Davis Center for Companion Animal Health","award":["111113"],"award-info":[{"award-number":["111113"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Our laboratory has serially reported on the virologic and immunopathologic features of a cohort of experimental feline immunodeficiency virus (FIV)-infected cats for more than eight years. At 8.09 years post infection (PI), one of these animals entered the terminal stage of infection, characterized by undulating hyperthermia, progressive anorexia, weight loss, and pancytopenia; the animal was not responsive to therapeutic interventions, necessitating euthanasia six weeks later (8.20 years PI). Subsequent analyses indicated that neoplastic lymphocytes infiltrated multiple cervical lymph nodes and a band-like region of the mucosal lamina propria within a segment of the intestine. Immunohistochemistry and T cell clonality testing determined that the nodal and intestinal lesions were independently arising from CD3 T cell lymphomas. In-situ RNA hybridization studies indicated that diffuse neoplastic lymphocytes from the cervical lymph node contained abundant viral nucleic acid, while viral nucleic acid was not detectable in lymphocytes from the intestinal lymphoma lesion. The proviral long terminal repeat (LTR) was amplified and sequenced from multiple anatomic sites, and a common clone containing a single nucleotide polymorphism was determined to be defective in response to phorbol myristate acetate (PMA)-mediated promoter activation in a reporter gene assay. This assay revealed a previously unidentified PMA response element within the FIV U3 region 3\u2019 to the TATA box. The possible implications of these results on FIV-lymphoma pathogenesis are discussed.<\/jats:p>","DOI":"10.3390\/v10060280","type":"journal-article","created":{"date-parts":[[2018,5,24]],"date-time":"2018-05-24T07:54:01Z","timestamp":1527148441000},"page":"280","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Multiple, Independent T Cell Lymphomas Arising in an Experimentally FIV-Infected Cat during the Terminal Stage of Infection"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0057-0604","authenticated-orcid":false,"given":"Brian","family":"Murphy","sequence":"first","affiliation":[{"name":"Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616-5270, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christina","family":"Eckstrand","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99163, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diego","family":"Castillo","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616-5270, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andre","family":"Poon","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616-5270, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Molly","family":"Liepnieks","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616-5270, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristy","family":"Harmon","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616-5270, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Moore","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616-5270, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2018,5,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1292\/jvms1939.52.645","article-title":"Clinical staging of feline immunodeficiency virus infection","volume":"52","author":"Ishida","year":"1990","journal-title":"Nihon Juigaku Zasshi"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1093\/infdis\/170.3.543","article-title":"Development of clinical disease in cats experimentally infected with feline immunodeficiency virus","volume":"170","author":"English","year":"1994","journal-title":"J. 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