{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T04:30:47Z","timestamp":1772253047197,"version":"3.50.1"},"reference-count":40,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2018,8,20]],"date-time":"2018-08-20T00:00:00Z","timestamp":1534723200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003176","name":"Ministerio de Educaci\u00f3n, Cultura y Deporte","doi-asserted-by":"publisher","award":["SAF 2006-12784-C02-01 and SAF2006-12784-C02-02"],"award-info":[{"award-number":["SAF 2006-12784-C02-01 and SAF2006-12784-C02-02"]}],"id":[{"id":"10.13039\/501100003176","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>In the context of long-term screening for viruses on Western Palaearctic bats, we tested for the presence of adenovirus 1392 oropharyngeal swabs and 325 stool samples taken from 27 bat species. Adenoviruses were detected in 12 species of the Vespertilionidae and the Rhinolophidae families. Fifty positive respiratory and 26 positive stool samples were studied. Phylogenetic analyses of partial hexon protein and partial DNA-dependent DNA polymerase genes indicate that all these bat adenoviruses belong to the genus Mastadenovirus but without constituting a monophyletic cluster. According to genetic identities, the new groups are distinct to the previously described Bat mastadenovirus A and B species and contribute with potentially new members. Our data support that diversity of bat mastadenovirus is host-dependent and increase the knowledge of potentially pathogenic virus from bats. Due to the active role of bats as viral reservoirs, the characterization of these viruses is relevant for Public Health.<\/jats:p>","DOI":"10.3390\/v10080443","type":"journal-article","created":{"date-parts":[[2018,8,20]],"date-time":"2018-08-20T11:23:06Z","timestamp":1534764186000},"page":"443","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["New Adenovirus Groups in Western Palaearctic Bats"],"prefix":"10.3390","volume":"10","author":[{"given":"Maria","family":"Iglesias-Caballero","sequence":"first","affiliation":[{"name":"Centro Nacional de Microbiolog\u00eda, Instituto de Salud Carlos III, Carretera de Majadahonda-Pozuelo km 2. Majadahonda 28220, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1383-8462","authenticated-orcid":false,"given":"Javier","family":"Juste","sequence":"additional","affiliation":[{"name":"Estaci\u00f3n Biol\u00f3gica de Do\u00f1ana, CSIC, Avda Am\u00e9rico Vespucio 16, 41092 Seville, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0977-741X","authenticated-orcid":false,"given":"Sonia","family":"V\u00e1zquez-Mor\u00f3n","sequence":"additional","affiliation":[{"name":"Centro Nacional de Microbiolog\u00eda, Instituto de Salud Carlos III, Carretera de Majadahonda-Pozuelo km 2. Majadahonda 28220, Madrid, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica Epidemiolog\u00eda y Salud P\u00fablica, CIBERESP, 28029 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Falcon","sequence":"additional","affiliation":[{"name":"Centro Nacional de Microbiolog\u00eda, Instituto de Salud Carlos III, Carretera de Majadahonda-Pozuelo km 2. Majadahonda 28220, Madrid, Spain"},{"name":"Consorcio Centro de Investigaci\u00f3n Biom\u00e9dica en Red (CIBER), 28029 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolina","family":"Aznar-Lopez","sequence":"additional","affiliation":[{"name":"Centro Nacional de Microbiolog\u00eda, Instituto de Salud Carlos III, Carretera de Majadahonda-Pozuelo km 2. Majadahonda 28220, Madrid, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica Epidemiolog\u00eda y Salud P\u00fablica, CIBERESP, 28029 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1181-7641","authenticated-orcid":false,"given":"Carlos","family":"Ib\u00e1\u00f1ez","sequence":"additional","affiliation":[{"name":"Estaci\u00f3n Biol\u00f3gica de Do\u00f1ana, CSIC, Avda Am\u00e9rico Vespucio 16, 41092 Seville, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1828-733X","authenticated-orcid":false,"given":"Francisco","family":"Pozo","sequence":"additional","affiliation":[{"name":"Centro Nacional de Microbiolog\u00eda, Instituto de Salud Carlos III, Carretera de Majadahonda-Pozuelo km 2. Majadahonda 28220, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guillermo","family":"Ruiz","sequence":"additional","affiliation":[{"name":"Centro Nacional de Microbiolog\u00eda, Instituto de Salud Carlos III, Carretera de Majadahonda-Pozuelo km 2. Majadahonda 28220, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose M.","family":"Berciano","sequence":"additional","affiliation":[{"name":"Centro Nacional de Microbiolog\u00eda, Instituto de Salud Carlos III, Carretera de Majadahonda-Pozuelo km 2. Majadahonda 28220, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Inazio","family":"Garin","sequence":"additional","affiliation":[{"name":"Department of Zoology and Animal Cell Biology, University of the Basque Country (UPV\/EHU), Leioa 48940, Basque Country, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joxerra","family":"Aihartza","sequence":"additional","affiliation":[{"name":"Department of Zoology and Animal Cell Biology, University of the Basque Country (UPV\/EHU), Leioa 48940, Basque Country, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan E.","family":"Echevarr\u00eda","sequence":"additional","affiliation":[{"name":"Centro Nacional de Microbiolog\u00eda, Instituto de Salud Carlos III, Carretera de Majadahonda-Pozuelo km 2. 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Rev."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Wong, S., Lau, S., Woo, P., and Yuen, K.Y. (2007). Bats as a continuing source of emerging infections in humans. Rev. Med. Virol.","DOI":"10.1002\/rmv.520"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"449","DOI":"10.3201\/eid1703.100526","article-title":"Amplification of emerging viruses in a bat colony","volume":"17","author":"Drexler","year":"2011","journal-title":"Emerg. Infect. Dis."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.tvjl.2010.06.020","article-title":"Novel adenoviruses and herpesviruses detected in bats","volume":"189","author":"Vidovszky","year":"2011","journal-title":"Vet. J."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1625","DOI":"10.1099\/jgv.0.000470","article-title":"Novel bat adenoviruses with an extremely large E3 gene","volume":"97","author":"Tan","year":"2016","journal-title":"J. Gen. Virol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1007\/s12250-016-3930-2","article-title":"Molecular detection of viruses in Kenyan bats and discovery of novel astroviruses, caliciviruses and rotaviruses","volume":"32","author":"Waruhiu","year":"2017","journal-title":"Virol. Sin."},{"key":"ref_8","unstructured":"Harrach, B., Benk\u00f6, M., Both, G., Brown, M., Davison, A.J., Echavarr\u00eda, M., Hess, M., Jones, M.S., Kajon, A., and Lehmkuhl, A.D. (2011). Family Adenoviridae. Virus Taxonomy: Classification and Nomenclature of Viruses. Ninth report of the International Committee of Taxonomy of Viruses, Elsevier."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"347","DOI":"10.3201\/eid1402.070932","article-title":"Isolation of novel adenovirus from fruit bat (Pteropus dasymallus yayeyamae)","volume":"14","author":"Maeda","year":"2008","journal-title":"Emerg. Infect. Dis."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2052","DOI":"10.3201\/eid1512.090646","article-title":"New adenovirus in bats, Germany","volume":"15","author":"Sonntag","year":"2009","journal-title":"Emerg. Infect. Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1888","DOI":"10.1128\/JVI.05974-11","article-title":"Genome analysis of bat adenovirus 2: Indications of interspecies transmission","volume":"86","author":"Kohl","year":"2012","journal-title":"J. Virol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"3889","DOI":"10.1128\/JVI.02497-09","article-title":"Host range, prevalence, and genetic diversity of adenoviruses in bats","volume":"84","author":"Li","year":"2010","journal-title":"J. Virol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1007\/s11262-013-0947-6","article-title":"First detection of adenovirus in the vampire bat (Desmodus rotundus) in Brazil","volume":"47","author":"Lima","year":"2013","journal-title":"Virus Genes"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1556\/004.2015.047","article-title":"Random sampling of the Central European bat fauna reveals the existence of numerous hitherto unknown adenoviruses","volume":"63","author":"Vidovszky","year":"2015","journal-title":"Acta Vet. Hung."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1099\/jgv.0.000739","article-title":"Novel bat adenoviruses with low G + C content shed new light on the evolution of adenoviruses","volume":"98","author":"Tan","year":"2017","journal-title":"J. Gen. Virol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.virol.2013.03.014","article-title":"Metagenomic study of the viruses of African straw-coloured fruit bats: Detection of a chiropteran poxvirus and isolation of a novel adenovirus","volume":"441","author":"Baker","year":"2013","journal-title":"Virology"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Ogawa, H., Kajihara, M., Nao, N., Shigeno, A., Fujikura, D., Hang\u2019ombe, B.M., Mweene, A.S., Mutemwa, A., Squarre, D., and Yamada, M. (2017). Characterization of a Novel Bat Adenovirus Isolated from Straw-Colored Fruit Bat (Eidolon helvum). Viruses, 9.","DOI":"10.3390\/v9120371"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"9584","DOI":"10.1038\/s41598-018-27836-w","article-title":"A novel adenovirus isolated from the Egyptian fruit bat in South Africa is closely related to recent isolates from China","volume":"8","author":"Allam","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Hackenbrack, N., Rogers, M.B., Ashley, R.E., Keel, M.K., Kubiski, S.V., Bryan, J.A., Ghedin, E., Holmes, E.C., Hafenstein, S.L., and Allison, A.B. (2017). Evolution and Cryo-electron Microscopy Capsid Structure of a North American Bat Adenovirus and Its Relationship to Other Mastadenoviruses. J. Virol., 91.","DOI":"10.1128\/JVI.01504-16"},{"key":"ref_20","first-page":"1030","article-title":"Rabies in Spain. A Peculiarity in Eurasia","volume":"3","author":"Berciano","year":"2017","journal-title":"Ann. Virol. Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1038\/hdy.2009.47","article-title":"Mitochondrial DNA signatures at different spatial scales: From the effects of the Straits of Gibraltar to population structure in the meridional serotine bat (Eptesicus isabellinus)","volume":"103","author":"Juste","year":"2009","journal-title":"Heredity"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"520","DOI":"10.3201\/eid1703.100894","article-title":"Phylogeny of European Bat Lyssavirus 1 in Eptesicus isabellinus Bats, Spain","volume":"17","author":"Juste","year":"2011","journal-title":"Emerg. Infect. Dis."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1263","DOI":"10.3201\/1408.080068","article-title":"Endemic Circulation of European Bat Lyssavirus Type 1 in Serotine Bats, Spain","volume":"14","author":"Juste","year":"2008","journal-title":"Emerg. Infect. Dis."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"793","DOI":"10.3201\/eid1905.121071","article-title":"Novel Lyssavirus in Bat, Spain","volume":"19","author":"Ceballos","year":"2013","journal-title":"Emerg. Infect. Dis."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Negredo, A., Palacios, G., V\u00e1zquez-Mor\u00f3n, S., Gonz\u00e1lez, F., Dopazo, H., Molero, F., Juste, J., Quetglas, J., Savji, N., and de la Cruz Mart\u00ednez, M. (2011). Discovery of an ebolavirus-like filovirus in europe. PLoS Pathog., 7.","DOI":"10.1371\/journal.ppat.1002304"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1883","DOI":"10.1007\/s00705-011-1057-1","article-title":"Detection of alpha and betacoronaviruses in multiple Iberian bat species","volume":"156","author":"Casas","year":"2011","journal-title":"Arch. Virol."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Pozo, F., Juste, J., V\u00e1zquez-Mor\u00f3n, S., Aznar-L\u00f3pez, C., Ib\u00e1\u00f1ez, C., Garin, I., Aihartza, J., Casas, I., Tenorio, A., and Echevarr\u00eda, J.E. (2016). Identification of Novel Betaherpesviruses in Iberian Bats Reveals Parallel Evolution. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0169153"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1099\/vir.0.046490-0","article-title":"Detection of rhabdovirus viral RNA in oropharyngeal swabs and ectoparasites of Spanish bats","volume":"94","author":"Marston","year":"2013","journal-title":"J. Gen. Virol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/0166-0934(94)00173-E","article-title":"New method for the extraction of viral RNA and DNA from cerebrospinal fluid for use in the polymerase chain reaction assay","volume":"53","author":"Casas","year":"1995","journal-title":"J. Virol. Methods"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Calvo, C., Garc\u00eda-Garc\u00eda, M.L., Sanchez-Dehesa, R., Rom\u00e1n, C., Tabares, A., Pozo, F., and Casas, I. (2015). Eight Year Prospective Study of Adenoviruses Infections in Hospitalized Children. Comparison with Other Respiratory Viruses. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0132162"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"6176","DOI":"10.1128\/JCM.43.12.6176-6182.2005","article-title":"Molecular Identification of Adenoviruses in Clinical Samples by Analyzing a Partial Hexon Genomic Region","volume":"43","author":"Casas","year":"2005","journal-title":"J. Clin. Microbiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1093\/bioinformatics\/14.9.817","article-title":"MODELTEST: Testing the model of DNA substitution","volume":"14","author":"Posada","year":"1998","journal-title":"Bioinformatics"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1111\/j.1095-8312.2008.01128.x","article-title":"The Straits of Gibraltar: Barrier or bridge to Ibero-Moroccan bat diversity?","volume":"96","author":"Juste","year":"2009","journal-title":"Biol. J. Linn. Soc."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"12125","DOI":"10.1128\/JVI.79.19.12125-12131.2005","article-title":"Adenovirus Receptors","volume":"79","author":"Zhang","year":"2005","journal-title":"J. Virol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1007\/978-1-4614-0980-9_12","article-title":"Viral Polymerases","volume":"726","author":"Choi","year":"2012","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1038\/nature04721","article-title":"Hexon-chimaeric adenovirus serotype 5 vectors circumvent pre-existing anti-vector immunity","volume":"441","author":"Roberts","year":"2006","journal-title":"Nature"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"9553","DOI":"10.1128\/JVI.77.17.9553-9566.2003","article-title":"Structural and phylogenetic analysis of adenovirus hexons by use of high-resolution X-ray crystallographic, molecular modeling, and sequence-based methods","volume":"77","author":"Rux","year":"2003","journal-title":"J. Virol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1494","DOI":"10.1128\/JCM.02289-09","article-title":"Molecular Characterization of an Adenovirus 3\u201316 Intertypic Recombinant Isolated in Argentina from an Infant Hospitalized with Acute Respiratory Infection","volume":"48","author":"Kajon","year":"2010","journal-title":"J. Clin. Microbiol."},{"key":"ref_39","unstructured":"Arroyo-Cabrales, J., and \u00c1lvarez-Casta\u00f1eda, S.T. (2017). Corynorhinus rafinesquii. The IUCN Red List of Threatened Species 2017, International Union for Conservation of Nature."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.ympev.2006.06.019","article-title":"Molecular phylogeny of New World Myotis (Chiroptera, Vespertilionidae) inferred from mitochondrial and nuclear DNA genes","volume":"43","author":"Stadelmann","year":"2007","journal-title":"Mol. Phylogenet. Evol."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/10\/8\/443\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T15:19:43Z","timestamp":1760195983000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/10\/8\/443"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,8,20]]},"references-count":40,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2018,8]]}},"alternative-id":["v10080443"],"URL":"https:\/\/doi.org\/10.3390\/v10080443","relation":{"has-preprint":[{"id-type":"doi","id":"10.20944\/preprints201807.0553.v1","asserted-by":"object"}]},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,8,20]]}}}