{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T16:06:29Z","timestamp":1789920389750,"version":"4.0.1"},"reference-count":22,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2018,11,14]],"date-time":"2018-11-14T00:00:00Z","timestamp":1542153600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Over recent years, there has been increasing interest in the use of the anelloviruses, the major component of the human virome, for the prediction of post-transplant complications such as severe infections. Due to an important diversity, the comprehensive characterization of this viral family over time has been poorly studied. To overcome this challenge, we used a metagenomic next-generation sequencing (mNGS) approach with the aim of determining the individual anellovirus profile of autologous stem cell transplant (ASCT) patients. We conducted a prospective pilot study on a homogeneous patient cohort regarding the chemotherapy regimens that included 10 ASCT recipients. A validated viral mNGS workflow was used on 108 plasma samples collected at 11 time points from diagnosis to 90 days post-transplantation. A complex interindividual variability in terms of abundance and composition was noticed. In particular, a strong sex effect was found and confirmed using quantitative PCR targeting torque teno virus, the most abundant anellovirus. Interestingly, an important turnover in the anellovirus composition was observed during the course of the disease revealing a strong intra-individual variability. Although more studies are needed to better understand anellovirus dynamics, these findings are of prime importance for their future use as biomarkers of immune competence.<\/jats:p>","DOI":"10.3390\/v10110633","type":"journal-article","created":{"date-parts":[[2018,11,14]],"date-time":"2018-11-14T10:58:22Z","timestamp":1542193102000},"page":"633","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Metagenomic Next-Generation Sequencing Reveals Individual Composition and Dynamics of Anelloviruses during Autologous Stem Cell Transplant Recipient Management"],"prefix":"10.3390","volume":"10","author":[{"given":"Antonin","family":"Bal","sequence":"first","affiliation":[{"name":"Laboratoire de Virologie, Institut des Agents Infectieux, Groupement Hospitalier Nord, Hospices Civils de Lyon, 69004 Lyon, France"},{"name":"Univ Lyon, Universit\u00e9 Lyon 1, Facult\u00e9 de M\u00e9decine Lyon Est, CIRI, Inserm U1111 CNRS UMR5308, Virpath, 69372 Lyon, France"},{"name":"Laboratoire Commun de Recherche HCL-bioMerieux, Centre Hospitalier Lyon Sud, 69495 Pierre-B\u00e9nite, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cl\u00e9mentine","family":"Sarkozy","sequence":"additional","affiliation":[{"name":"Service d\u2019H\u00e9matologie Clinique, Centre Hospitalier Lyon Sud, Pierre-B\u00e9nite, 69495 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7158-1186","authenticated-orcid":false,"given":"Laurence","family":"Josset","sequence":"additional","affiliation":[{"name":"Laboratoire de Virologie, Institut des Agents Infectieux, Groupement Hospitalier Nord, Hospices Civils de Lyon, 69004 Lyon, France"},{"name":"Univ Lyon, Universit\u00e9 Lyon 1, Facult\u00e9 de M\u00e9decine Lyon Est, CIRI, Inserm U1111 CNRS UMR5308, Virpath, 69372 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9rie","family":"Cheynet","sequence":"additional","affiliation":[{"name":"Laboratoire Commun de Recherche HCL-bioMerieux, Centre Hospitalier Lyon Sud, 69495 Pierre-B\u00e9nite, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guy","family":"Oriol","sequence":"additional","affiliation":[{"name":"Laboratoire Commun de Recherche HCL-bioMerieux, Centre Hospitalier Lyon Sud, 69495 Pierre-B\u00e9nite, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9r\u00e9mie","family":"Becker","sequence":"additional","affiliation":[{"name":"Bioaster, Genomics and Transcriptomics Technological Unit, 69007 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ga\u00eblle","family":"Vilchez","sequence":"additional","affiliation":[{"name":"Laboratoire Commun de Recherche HCL-bioMerieux, Centre Hospitalier Lyon Sud, 69495 Pierre-B\u00e9nite, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Sesques","sequence":"additional","affiliation":[{"name":"Service d\u2019H\u00e9matologie Clinique, Centre Hospitalier Lyon Sud, Pierre-B\u00e9nite, 69495 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Mallet","sequence":"additional","affiliation":[{"name":"Laboratoire Commun de Recherche HCL-bioMerieux, Centre Hospitalier Lyon Sud, 69495 Pierre-B\u00e9nite, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre","family":"Pachot","sequence":"additional","affiliation":[{"name":"Laboratoire Commun de Recherche HCL-bioMerieux, Centre Hospitalier Lyon Sud, 69495 Pierre-B\u00e9nite, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Morfin","sequence":"additional","affiliation":[{"name":"Laboratoire de Virologie, Institut des Agents Infectieux, Groupement Hospitalier Nord, Hospices Civils de Lyon, 69004 Lyon, France"},{"name":"Univ Lyon, Universit\u00e9 Lyon 1, Facult\u00e9 de M\u00e9decine Lyon Est, CIRI, Inserm U1111 CNRS UMR5308, Virpath, 69372 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8959-2123","authenticated-orcid":false,"given":"Bruno","family":"Lina","sequence":"additional","affiliation":[{"name":"Laboratoire de Virologie, Institut des Agents Infectieux, Groupement Hospitalier Nord, Hospices Civils de Lyon, 69004 Lyon, France"},{"name":"Univ Lyon, Universit\u00e9 Lyon 1, Facult\u00e9 de M\u00e9decine Lyon Est, CIRI, Inserm U1111 CNRS UMR5308, Virpath, 69372 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9541-8666","authenticated-orcid":false,"given":"Gilles","family":"Salles","sequence":"additional","affiliation":[{"name":"Service d\u2019H\u00e9matologie Clinique, Centre Hospitalier Lyon Sud, Pierre-B\u00e9nite, 69495 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fr\u00e9deric","family":"Reynier","sequence":"additional","affiliation":[{"name":"Bioaster, Genomics and Transcriptomics Technological Unit, 69007 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Trouillet-Assant","sequence":"additional","affiliation":[{"name":"Univ Lyon, Universit\u00e9 Lyon 1, Facult\u00e9 de M\u00e9decine Lyon Est, CIRI, Inserm U1111 CNRS UMR5308, Virpath, 69372 Lyon, France"},{"name":"Laboratoire Commun de Recherche HCL-bioMerieux, Centre Hospitalier Lyon Sud, 69495 Pierre-B\u00e9nite, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen","family":"Brengel-Pesce","sequence":"additional","affiliation":[{"name":"Laboratoire Commun de Recherche HCL-bioMerieux, Centre Hospitalier Lyon Sud, 69495 Pierre-B\u00e9nite, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,11,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Moreto, A., Fari\u00f1as-Alvarez, C., Puente, M., Ocejo-Vinyals, J.G., S\u00e1nchez-Velasco, P., Horcajada, J.P., Batlle, A., Montes, C., Santos, F., and Conde, E. 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