{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T04:07:54Z","timestamp":1790741274063,"version":"4.1.0"},"reference-count":45,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2018,11,17]],"date-time":"2018-11-17T00:00:00Z","timestamp":1542412800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL114152"],"award-info":[{"award-number":["HL114152"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL130871"],"award-info":[{"award-number":["HL130871"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011338","name":"Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research, Ministry of Education","doi-asserted-by":"publisher","award":["No. 313033"],"award-info":[{"award-number":["No. 313033"]}],"id":[{"id":"10.13039\/501100011338","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Adeno associated virus (AAV) is a versatile gene delivery tool, which has been approved as a human gene therapy vector for combating genetic diseases. AAV capsid proteins are the major components that determine the tissue specificity, immunogenicity and in vivo transduction performance of the vector. In this study, the AAV8 capsid glycosylation profile was systemically analyzed by peptide mass fingerprinting utilizing high-resolution mass spectrometry to determine the presence of capsid glycosylation. We identified N-glycosylation on the amino acid N499 of the capsid protein. We characterized the overall sugar profile for vector produced in 293 cells. Multiple N-glycosylated host-cell proteins (HCPs) copurified with AAV8 vectors and were identified by analyzing LC-MS data utilizing a human database and proteome discoverer search engine. The N-glycosylation analysis by MALDI-TOF MS, highlighted the probability of AAV8 interaction with terminal galactosylated N-glycans within the HCPs.<\/jats:p>","DOI":"10.3390\/v10110644","type":"journal-article","created":{"date-parts":[[2018,11,23]],"date-time":"2018-11-23T03:41:31Z","timestamp":1542944491000},"page":"644","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["Site-Specific N-Glycosylation on the AAV8 Capsid Protein"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8757-2746","authenticated-orcid":false,"given":"Arya","family":"Aloor","sequence":"first","affiliation":[{"name":"Center for Diagnostics &amp; Therapeutics and Department of Chemistry, Georgia State University, Atlanta, GA 30302, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junping","family":"Zhang","sequence":"additional","affiliation":[{"name":"Sol Sherry Thrombosis Research Center, Lewis Katz, School of Medicine, Temple University, Philadelphia, PA 19140, USA"},{"name":"School of Biomedical Science, Huaqiao University, Quanzhou 362021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ebtesam A.","family":"Gashash","sequence":"additional","affiliation":[{"name":"Center for Diagnostics &amp; Therapeutics and Department of Chemistry, Georgia State University, Atlanta, GA 30302, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aishwarya","family":"Parameswaran","sequence":"additional","affiliation":[{"name":"Center for Diagnostics &amp; Therapeutics and Department of Chemistry, Georgia State University, Atlanta, GA 30302, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Chrzanowski","sequence":"additional","affiliation":[{"name":"Sol Sherry Thrombosis Research Center, Lewis Katz, School of Medicine, Temple University, Philadelphia, PA 19140, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Ma","sequence":"additional","affiliation":[{"name":"Center for Diagnostics &amp; Therapeutics and Department of Chemistry, Georgia State University, Atlanta, GA 30302, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Diao","sequence":"additional","affiliation":[{"name":"School of Biomedical Science, Huaqiao University, Quanzhou 362021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng George","family":"Wang","sequence":"additional","affiliation":[{"name":"Center for Diagnostics &amp; Therapeutics and Department of Chemistry, Georgia State University, Atlanta, GA 30302, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weidong","family":"Xiao","sequence":"additional","affiliation":[{"name":"Sol Sherry Thrombosis Research Center, Lewis Katz, School of Medicine, Temple University, Philadelphia, PA 19140, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,11,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"858","DOI":"10.1038\/gt.2008.68","article-title":"Clinical gene therapy using recombinant adeno-associated virus vectors","volume":"15","author":"Mueller","year":"2008","journal-title":"Gene Ther."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1128\/CMR.00008-08","article-title":"Gene therapy using adeno-associated virus vectors","volume":"21","author":"Daya","year":"2008","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"JVI.00391-17","DOI":"10.1128\/JVI.00391-17","article-title":"AAV serotypes have distinctive interactions with domains of the cellular receptor AAVR","volume":"91","author":"Pillay","year":"2017","journal-title":"J. Virol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/JVI.01980-16","article-title":"Adeno-Associated Virus Assembly-Activating Protein Is Not an Essential Requirement for Capsid Assembly of AAV Serotypes 4, 5 and 11","volume":"91","author":"Earley","year":"2017","journal-title":"J. Virol."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Vance, M.A., Mitchell, A., and Samulski, R.J. (2015). AAV Biology, Infectivity and Therapeutic Use from Bench to Clinic. Gene Ther. Princ. Chall.","DOI":"10.5772\/61988"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"12686","DOI":"10.1128\/JVI.05359-11","article-title":"The Assembly-Activating Protein Promotes Capsid Assembly of Different Adeno-Associated Virus Serotypes","volume":"85","author":"Sonntag","year":"2011","journal-title":"J. Virol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"10220","DOI":"10.1073\/pnas.1001673107","article-title":"A viral assembly factor promotes AAV2 capsid formation in the nucleolus","volume":"107","author":"Sonntag","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"3356","DOI":"10.1128\/jvi.62.9.3356-3363.1988","article-title":"Alternate mRNA Splicing Is Required for Synthesis of Adeno-Associated Virus VP1 Capsid Protein","volume":"62","author":"Trempe","year":"1988","journal-title":"J. Virol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"12260","DOI":"10.1128\/JVI.01304-07","article-title":"Structure of Adeno-Associated Virus Serotype 8, a Gene Therapy Vector","volume":"81","author":"Nam","year":"2007","journal-title":"J. Virol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"5296","DOI":"10.1128\/JVI.79.9.5296-5303.2005","article-title":"Von Der A Conformational Change in the Adeno-Associated Virus Type 2 Capsid Leads to the Exposure of Hidden VP1 N Termini","volume":"79","author":"Kronenberg","year":"2005","journal-title":"J. Virol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2991","DOI":"10.1128\/JVI.03371-13","article-title":"Differential Adeno-Associated Virus Serotype-Specific Interaction Patterns with Synthetic Heparins and Other Glycans","volume":"88","author":"Mietzsch","year":"2014","journal-title":"J. Virol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/j.ymthe.2004.05.024","article-title":"Recombinant AAV viral vectors pseudotyped with viral capsids from serotypes 1, 2, and 5 display differential efficiency and cell tropism after delivery to different regions of the central nervous system","volume":"10","author":"Burger","year":"2004","journal-title":"Mol. Ther."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"6081","DOI":"10.1073\/pnas.0937739100","article-title":"Adeno-associated viruses undergo substantial evolution in primates during natural infections","volume":"100","author":"Gao","year":"2003","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"6381","DOI":"10.1128\/JVI.78.12.6381-6388.2004","article-title":"Clades of Adeno-Associated Viruses Are Widely Disseminated in Human Tissues","volume":"78","author":"Gao","year":"2004","journal-title":"J. Virol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"13150","DOI":"10.1128\/JVI.01415-13","article-title":"Comparative Analysis of Adeno-Associated Virus Capsid Stability and Dynamics","volume":"87","author":"Rayaprolu","year":"2013","journal-title":"J. Virol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.ymthe.2004.10.004","article-title":"Efficacy of an adeno-associated virus 8-pseudotyped vector in glycogen storage disease type II","volume":"11","author":"Sun","year":"2005","journal-title":"Mol. Ther."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"9831","DOI":"10.1128\/JVI.00878-06","article-title":"The 37\/67-Kilodalton Laminin Receptor Is a Receptor for Adeno-Associated Virus Serotypes 8, 2, 3, and 9","volume":"80","author":"Akache","year":"2006","journal-title":"J. Virol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.1182\/blood-2003-08-2954","article-title":"Total correction of hemophilia A mice with canine FVIII using an AAV 8 serotype","volume":"103","author":"Sarkar","year":"2004","journal-title":"Blood"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1111\/j.1538-7836.2006.02220.x","article-title":"Efficacy and safety of adeno-associated viral vectors based on serotype 8 and 9 vs. lentiviral vectors for hemophilia B gene therapy","volume":"5","author":"Vandendriessche","year":"2007","journal-title":"J. Thromb. Haemost."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.1016\/j.ymthe.2017.03.033","article-title":"Gene Therapy for Hemophilia","volume":"25","author":"Nienhuis","year":"2017","journal-title":"Mol. Ther."},{"key":"ref_21","first-page":"2357","article-title":"Adenovirus-associated virus vector-mediated gene transfer in hemophilia B","volume":"365","author":"Chir","year":"2012","journal-title":"N. Engl. J. Med."},{"key":"ref_22","first-page":"1994","article-title":"Long-Term Safety and Efficacy of Factor IX Gene Therapy in Hemophilia B","volume":"374","author":"Nathwani","year":"2015","journal-title":"N. Engl. J. Med."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1002\/jgm.554","article-title":"Gene therapy with novel adeno-associated virus vectors substantially diminishes atherosclerosis in a murine model of familial hypercholesterolemia","volume":"6","author":"Lebherz","year":"2004","journal-title":"J. Gene Med."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1038\/nbt1073","article-title":"Adeno-associated virus serotype 8 efficiently delivers genes to muscle and heart","volume":"23","author":"Wang","year":"2005","journal-title":"Nat. Biotechnol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"6884","DOI":"10.1128\/JVI.75.15.6884-6893.2001","article-title":"Adeno-associated virus serotype 4 (AAV4) and AAV5 both require sialic acid binding for hemagglutination and efficient transduction but differ in sialic acid linkage specificity","volume":"75","author":"Kaludov","year":"2001","journal-title":"J. Virol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1438","DOI":"10.1128\/JVI.72.2.1438-1445.1998","article-title":"Membrane-associated heparan sulfate proteoglycan is a receptor for adeno-associated virus type 2 virions","volume":"72","author":"Summerford","year":"1998","journal-title":"J. Virol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"13532","DOI":"10.1074\/jbc.M110.210922","article-title":"Terminal N-linked galactose is the primary receptor for adeno-associated virus","volume":"286","author":"Shen","year":"2011","journal-title":"J. Biol. Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1128\/JVI.01219-07","article-title":"Mutations within Potential Glycosylation Sites in the Capsid Protein of Hepatitis E Virus Prevent the Formation of Infectious Virus Particles","volume":"82","author":"Graff","year":"2008","journal-title":"J. Virol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"5453","DOI":"10.1021\/bi047702b","article-title":"Identification of the Glycosylation Site of the Adenovirus Type 5 Fiber Protein","volume":"44","author":"Cauet","year":"2005","journal-title":"Biochemistry"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"7827","DOI":"10.1073\/pnas.0802866105","article-title":"Next generation of adeno-associated virus 2 vectors: Point mutations in tyrosines lead to high-efficiency transduction at lower doses","volume":"105","author":"Zhong","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1412","DOI":"10.1038\/nprot.2006.207","article-title":"Production and characterization of adeno-associated viral vectors","volume":"1","author":"Grieger","year":"2006","journal-title":"Nat. Protoc."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2856","DOI":"10.1038\/nprot.2006.468","article-title":"Nature In-gel Digestion Protocol","volume":"1","author":"Shevchenko","year":"2007","journal-title":"Ref. Nat. Protoc."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1089\/hgtb.2016.178","article-title":"Direct Liquid Chromatography\/Mass Spectrometry Analysis for Complete Characterization of Recombinant Adeno-Associated Virus Capsid Proteins","volume":"28","author":"Jin","year":"2017","journal-title":"Hum. Gene Ther. Methods"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ab.2005.09.037","article-title":"Advances in fluorescence derivatization methods for high-performance liquid chromatographic analysis of glycoprotein carbohydrates","volume":"1","author":"Anumula","year":"2006","journal-title":"Anal. Biochem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1853","DOI":"10.1016\/j.bbapap.2006.10.007","article-title":"Strategies for analysis of glycoprotein glycosylation","volume":"1764","author":"Geyer","year":"2006","journal-title":"Biochim. Biophys. Acta-Proteins Proteom."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2440","DOI":"10.1002\/elps.201200662","article-title":"N-linked glycoproteome profiling of human serum using tandem enrichment and multiple fraction concatenation","volume":"34","author":"Ma","year":"2013","journal-title":"Electrophoresis"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"3348","DOI":"10.1021\/acs.jproteome.7b00359","article-title":"An Insight into Glyco-Microheterogeneity of Plasma von Willebrand Factor by Mass Spectrometry","volume":"16","author":"Gashash","year":"2017","journal-title":"J. Proteome Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2985","DOI":"10.1002\/rcm.3173","article-title":"pFind 2.0: A software package for peptide and protein identification via tandem mass spectrometry","volume":"21","author":"Wang","year":"2007","journal-title":"Rapid Commun. Mass Spectrom."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1002\/elps.1150180314","article-title":"Detailed peptide characterization using PEPTIDEMASS\u2014A World-Wide-Web-accessible tool","volume":"18","author":"Wilkins","year":"1997","journal-title":"Electrophoresis"},{"key":"ref_40","unstructured":"Duncan, M., Fung, K., Wang, H., Yen, C., and Cios, K. (2003, January 11\u201314). Identification of contaminants in proteomics mass spectrometry data. Proceedings of the 2003 IEEE Bioinformatics Conference on Computational Systems Bioinformatics (CSB2003), Stanford, CA, USA."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1842","DOI":"10.1016\/j.jprot.2011.05.014","article-title":"Protein identification using MS\/MS data","volume":"74","author":"Cottrell","year":"2011","journal-title":"J. Proteom."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"6171","DOI":"10.1128\/JVI.02417-05","article-title":"Characterization of the Capsid Protein Glycosylation of Adeno-Associated Virus Type 2 by High-Resolution Mass Spectrometry","volume":"80","author":"Murray","year":"2006","journal-title":"J. Virol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"28814","DOI":"10.1074\/jbc.M113.482380","article-title":"Engraftment of a galactose receptor footprint onto adeno-associated viral capsids improves transduction efficiency","volume":"288","author":"Shen","year":"2013","journal-title":"J. Biol. Chem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"6620","DOI":"10.1128\/JVI.00297-12","article-title":"Human Galectin 3 Binding Protein Interacts with Recombinant Adeno-Associated Virus Type 6","volume":"86","author":"Denard","year":"2012","journal-title":"J. Virol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1660","DOI":"10.1128\/JVI.02503-14","article-title":"Identification and Mutagenesis of the Adeno-Associated Virus 5 Sialic Acid Binding Region","volume":"89","author":"Afione","year":"2015","journal-title":"J. Virol."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/10\/11\/644\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T15:30:19Z","timestamp":1760196619000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/10\/11\/644"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,11,17]]},"references-count":45,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2018,11]]}},"alternative-id":["v10110644"],"URL":"https:\/\/doi.org\/10.3390\/v10110644","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,11,17]]}}}