{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T16:55:46Z","timestamp":1790441746365,"version":"4.1.0"},"reference-count":63,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2018,11,22]],"date-time":"2018-11-22T00:00:00Z","timestamp":1542844800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["MOP-148689"],"award-info":[{"award-number":["MOP-148689"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Early region 1A (E1A) is the first viral protein produced upon human adenovirus (HAdV) infection. This multifunctional protein transcriptionally activates other HAdV early genes and reprograms gene expression in host cells to support productive infection. E1A functions by interacting with key cellular regulatory proteins through short linear motifs (SLiMs). In this study, the molecular determinants of interaction between E1A and BS69, a cellular repressor that negatively regulates E1A transactivation, were systematically defined by mutagenesis experiments. We found that a minimal sequence comprised of MPNLVPEV, which contains a conserved PXLXP motif and spans residues 112\u2013119 in HAdV-C5 E1A, was necessary and sufficient in binding to the myeloid, Nervy, and DEAF-1 (MYND) domain of BS69. Our study also identified residues P113 and L115 as critical for this interaction. Furthermore, the HAdV-C5 and -A12 E1A proteins from species C and A bound BS69, but those of HAdV-B3, -E4, -D9, -F40, and -G52 from species B, E, D, F, and G, respectively, did not. In addition, BS69 functioned as a repressor of E1A-mediated transactivation, but only for HAdV-C5 and HAdV-A12 E1A. Thus, the PXLXP motif present in a subset of HAdV E1A proteins confers interaction with BS69, which serves as a negative regulator of E1A mediated transcriptional activation.<\/jats:p>","DOI":"10.3390\/v10120662","type":"journal-article","created":{"date-parts":[[2018,11,23]],"date-time":"2018-11-23T03:41:31Z","timestamp":1542944491000},"page":"662","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["The Transcriptional Repressor BS69 is a Conserved Target of the E1A Proteins from Several Human Adenovirus Species"],"prefix":"10.3390","volume":"10","author":[{"given":"Ali","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, The University of Western Ontario, London, ON N6A 3K7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanner M.","family":"Tessier","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, The University of Western Ontario, London, ON N6A 3K7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4199-6874","authenticated-orcid":false,"given":"Kristianne J. C.","family":"Galpin","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, The University of Western Ontario, London, ON N6A 3K7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5531-5347","authenticated-orcid":false,"given":"Cason R.","family":"King","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, The University of Western Ontario, London, ON N6A 3K7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1328-6250","authenticated-orcid":false,"given":"Steven F.","family":"Gameiro","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, The University of Western Ontario, London, ON N6A 3K7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wyatt W.","family":"Anderson","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, The University of Western Ontario, London, ON N6A 3K7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed F.","family":"Yousef","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Khalifa University, 54224 Abu Dhabi, UAE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0636-8506","authenticated-orcid":false,"given":"Wen T.","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, The University of Western Ontario, London, ON N6A 3K7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shawn S. C.","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, The University of Western Ontario, London, ON N6A 3K7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joe S.","family":"Mymryk","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, The University of Western Ontario, London, ON N6A 3K7, Canada"},{"name":"Department of Oncology, The University of Western Ontario, London, ON N6A 3K7, Canada"},{"name":"London Regional Cancer Program, Lawson Health Research Institute, London, ON N6C 2R5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,11,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1128\/mr.51.4.419-430.1987","article-title":"Regulation of early adenovirus gene expression","volume":"51","author":"Nevins","year":"1987","journal-title":"Microbiol. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"7252","DOI":"10.1128\/JVI.00104-08","article-title":"Intrinsic structural disorder in adenovirus E1A: A viral molecular hub linking multiple diverse processes","volume":"82","author":"Pelka","year":"2008","journal-title":"J. Virol."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"King, C.R., Zhang, A., Tessier, T.M., Gameiro, S.F., and Mymryk, J.S. (2018). Hacking the Cell: Network Intrusion and Exploitation by Adenovirus E1A. MBio, 9.","DOI":"10.1128\/mBio.00390-18"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1038\/368520a0","article-title":"Promoter targeting by adenovirus E1A through interaction with different cellular DNA-binding domains","volume":"368","author":"Liu","year":"1994","journal-title":"Nature"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/S0065-230X(08)60995-1","article-title":"Transcriptional and transforming activities of the adenovirus E1A proteins","volume":"57","author":"Shenk","year":"1991","journal-title":"Adv. Cancer Res."},{"key":"ref_6","first-page":"1581","article-title":"Tumour suppressive properties of the adenovirus 5 E1A oncogene","volume":"13","author":"Mymryk","year":"1996","journal-title":"Oncogene"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"7824","DOI":"10.1038\/sj.onc.1204913","article-title":"Adenovirus E1A: Remodelling the host cell, a life or death experience","volume":"20","author":"Gallimore","year":"2001","journal-title":"Oncogene"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1038\/nrm827","article-title":"Adenovirus-5 E1A: Paradox and paradigm","volume":"3","author":"Frisch","year":"2002","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"King, C.R., Cohen, M.J., Fonseca, G.J., Dirk, B.S., Dikeakos, J.D., and Mymryk, J.S. (2016). Functional and Structural Mimicry of Cellular Protein Kinase A Anchoring Proteins by a Viral Oncoprotein. PLoS Pathog., 12.","DOI":"10.1371\/journal.ppat.1005621"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"7968","DOI":"10.1128\/JVI.76.16.7968-7975.2002","article-title":"Comparative sequence analysis of the largest E1A proteins of human and simian adenoviruses","volume":"76","author":"Avvakumov","year":"2002","journal-title":"J. Virol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1038\/338039a0","article-title":"Transcription activation by the adenovirus E1A protein","volume":"338","author":"Lillie","year":"1989","journal-title":"Nature"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1038\/346147a0","article-title":"Evidence for interaction of different eukaryotic transcriptional activators with distinct cellular targets","volume":"346","author":"Martin","year":"1990","journal-title":"Nature"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.tibs.2010.10.002","article-title":"How viruses hijack cell regulation","volume":"36","author":"Davey","year":"2011","journal-title":"Trends Biochem. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.tibs.2014.11.001","article-title":"How pathogens use linear motifs to perturb host cell networks","volume":"40","author":"Via","year":"2015","journal-title":"Trends Biochem. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"6733","DOI":"10.1021\/cr400585q","article-title":"Short linear motifs: ubiquitous and functionally diverse protein interaction modules directing cell regulation","volume":"114","author":"Uyar","year":"2014","journal-title":"Chem. Rev."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3342","DOI":"10.1016\/j.febslet.2005.04.005","article-title":"Linear motifs: evolutionary interaction switches","volume":"579","author":"Neduva","year":"2005","journal-title":"FEBS Lett."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1016\/j.virol.2008.12.010","article-title":"How the RB tumor suppressor structure and function was revealed by the study of Adenovirus and SV40","volume":"384","author":"DeCaprio","year":"2009","journal-title":"Virology"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"6799","DOI":"10.1128\/MCB.20.18.6799-6805.2000","article-title":"Role of the LXCXE binding site in RB function","volume":"20","author":"Dahiya","year":"2000","journal-title":"Mol. Cell Biol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"6893","DOI":"10.1128\/jvi.66.12.6893-6902.1992","article-title":"Homologous sequences in adenovirus E1A and human papillomavirus E7 proteins mediate interaction with the same set of cellular proteins","volume":"66","author":"Dyson","year":"1992","journal-title":"J. Virol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"4693","DOI":"10.1038\/onc.2010.226","article-title":"Identification of a molecular recognition feature in the E1A oncoprotein that binds the SUMO conjugase UBC9 and likely interferes with polySUMOylation","volume":"29","author":"Yousef","year":"2010","journal-title":"Oncogene"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1126\/science.aab3291","article-title":"DNA tumor virus oncogenes antagonize the cGAS-STING DNA-sensing pathway","volume":"350","author":"Lau","year":"2015","journal-title":"Science"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3159","DOI":"10.1002\/j.1460-2075.1995.tb07318.x","article-title":"BS69, a novel adenovirus E1A-associated protein that inhibits E1A transactivation","volume":"14","author":"Hateboer","year":"1995","journal-title":"EMBO J."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"4906","DOI":"10.1074\/jbc.M110078200","article-title":"The conserved MYND domain of BS69 binds cellular and oncoviral proteins through a common PXLXP motif","volume":"277","author":"Ansieau","year":"2002","journal-title":"J. Biol. Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1016\/j.virol.2004.08.007","article-title":"Comprehensive sequence analysis of the E1A proteins of human and simian adenoviruses","volume":"329","author":"Avvakumov","year":"2004","journal-title":"Virology"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Harter, M.R., Liu, C.-D., Shen, C.-L., Gonzalez-Hurtado, E., Zhang, Z.-M., Xu, M., Martinez, E., Peng, C.-W., and Song, J. (2016). BS69\/ZMYND11 C-Terminal Domains Bind and Inhibit EBNA2. PLoS Pathog., 12.","DOI":"10.1371\/journal.ppat.1005414"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1016\/j.molcel.2014.08.022","article-title":"BS69\/ZMYND11 reads and connects histone H3.3 lysine 36 trimethylation-decorated chromatin to regulated pre-mRNA processing","volume":"56","author":"Guo","year":"2014","journal-title":"Mol. Cell"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1038\/nature13045","article-title":"ZMYND11 links histone H3.3K36ME3 to transcription elongation and tumour suppression","volume":"508","author":"Wen","year":"2014","journal-title":"Nature"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"16546","DOI":"10.1074\/jbc.M600573200","article-title":"New insights into BS69 functions","volume":"281","author":"Velasco","year":"2006","journal-title":"J. Biol. Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1038\/cr.2014.38","article-title":"Crystal structure of human BS69 Bromo-ZnF-PWWP reveals its role in H3K36ME3 nucleosome binding","volume":"24","author":"Wang","year":"2014","journal-title":"Cell Res."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Kateb, F., Perrin, H., Tripsianes, K., Zou, P., Spadaccini, R., Bottomley, M., Franzmann, T.M., Buchner, J., Ansieau, S., and Sattler, M. (2013). Structural and functional analysis of the DEAF-1 and BS69 MYND domains. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0054715"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1911","DOI":"10.2741\/2197","article-title":"BS69, a corepressor interacting with ZHX1, is a bifunctional transcription factor","volume":"12","author":"Yamada","year":"2007","journal-title":"Front. Biosci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1042\/bj20030171","article-title":"Zinc-fingers and homeoboxes (ZHX) 2, a novel member of the ZHX family, functions as a transcriptional repressor","volume":"373","author":"Kawata","year":"2003","journal-title":"Biochem. J."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"26524","DOI":"10.1074\/jbc.M204121200","article-title":"M-Bop, a repressor protein essential for cardiogenesis, interacts with skNAC, a heart- and muscle-specific transcription factor","volume":"277","author":"Sims","year":"2002","journal-title":"J. Biol. Chem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"11928","DOI":"10.1093\/nar\/gku929","article-title":"ETS1 is a genome-wide effector of RAS\/ERK signaling in epithelial cells","volume":"42","author":"Plotnik","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"ref_35","first-page":"2173","article-title":"ETS-2 interacts with co-repressor BS69 to repress target gene expression","volume":"23","author":"Wei","year":"2003","journal-title":"Anticancer Res."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Plotnik, J.P., and Hollenhorst, P.C. (2017). Interaction with ZMYND11 mediates opposing roles of Ras-responsive transcription factors ETS1 and ETS2. Nucleic Acids Res., gkx039.","DOI":"10.1093\/nar\/gkx039"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/j.bbrc.2005.11.028","article-title":"Ubiquitin-dependent degradation of adenovirus E1A protein is inhibited by BS69","volume":"339","author":"Isobe","year":"2006","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1038\/sj.onc.1204048","article-title":"BS69, an adenovirus E1A-associated protein, inhibits the transcriptional activity of c-MYB","volume":"20","author":"Ladendorff","year":"2001","journal-title":"Oncogene"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1538","DOI":"10.1038\/sj.onc.1203421","article-title":"The adenovirus E1A binding protein BS69 is a corepressor of transcription through recruitment of N-CoR","volume":"19","author":"Masselink","year":"2000","journal-title":"Oncogene"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1091\/mbc.12.3.699","article-title":"Interaction of the E1A oncoprotein with Yak1p, a novel regulator of yeast pseudohyphal differentiation, and related mammalian kinases","volume":"12","author":"Zhang","year":"2001","journal-title":"Mol. Biol. Cell"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/0378-1119(89)90358-2","article-title":"Site-directed mutagenesis by overlap extension using the polymerase chain reaction","volume":"77","author":"Ho","year":"1989","journal-title":"Gene"},{"key":"ref_42","unstructured":"Adams, A., Gottschling, D.E., and Kaiser, C.A. (1997). Methods in Yeast Genetics, Cold Spring Harbor Press."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1002\/yea.320110408","article-title":"Studies on the transformation of intact yeast cells by the LiAc\/SS-DNA\/PEG procedure","volume":"11","author":"Gietz","year":"1995","journal-title":"Yeast"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Von der Haar, T. (2007). Optimized protein extraction for quantitative proteomics of yeasts. PLoS ONE, 2.","DOI":"10.1371\/journal.pone.0001078"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.virol.2003.08.005","article-title":"The E1A proteins of all six human adenovirus subgroups target the p300\/CBP acetyltransferases and the SAGA transcriptional regulatory complex","volume":"316","author":"Shuen","year":"2003","journal-title":"Virology"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"37868","DOI":"10.1074\/jbc.M504877200","article-title":"Molecular Determinants for the Complex Formation between the Retinoblastoma Protein and LXCXE Sequences","volume":"280","author":"Singh","year":"2005","journal-title":"J. Biol. Chem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1126\/science.1411535","article-title":"E2F: A link between the RB tumor suppressor protein and viral oncoproteins","volume":"258","author":"Nevins","year":"1992","journal-title":"Science"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2603","DOI":"10.1002\/j.1460-2075.1992.tb05325.x","article-title":"Adenovirus E1A prevents the retinoblastoma gene product from repressing the activity of a cellular transcription factor","volume":"11","author":"Zamanian","year":"1992","journal-title":"EMBO J."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"2796","DOI":"10.1038\/sj.onc.1208619","article-title":"Pocket proteins and cell cycle control","volume":"24","author":"Cobrinik","year":"2005","journal-title":"Oncogene"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"2173","DOI":"10.1242\/jcs.01227","article-title":"Molecular mechanisms of E2F-dependent activation and pRB-mediated repression","volume":"117","author":"Frolov","year":"2004","journal-title":"J. Cell Sci."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1016\/0092-8674(90)90112-R","article-title":"Adenovirus E1A proteins can dissociate heteromeric complexes involving the E2F transcription factor: A novel mechanism for E1A trans-activation","volume":"62","author":"Bagchi","year":"1990","journal-title":"Cell"},{"key":"ref_52","first-page":"2653","article-title":"E1A 13S and 12S mRNA products made in Escherichia coli both function as nucleus-localized transcription activators but do not directly bind DNA","volume":"5","author":"Ferguson","year":"1985","journal-title":"Mol. Cell Biol."},{"key":"ref_53","first-page":"4287","article-title":"Trans-dominant mutants of E1A provide genetic evidence that the zinc finger of the trans-activating domain binds a transcription factor","volume":"11","author":"Webster","year":"1991","journal-title":"Mol. Cell Biol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1038\/20466","article-title":"Mammalian SRB\/Mediator complex is targeted by adenovirus E1A protein","volume":"399","author":"Boyer","year":"1999","journal-title":"Nature"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"12003","DOI":"10.1073\/pnas.2035253100","article-title":"Activation domain-mediator interactions promote transcription preinitiation complex assembly on promoter DNA","volume":"100","author":"Cantin","year":"2003","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"2488","DOI":"10.1073\/pnas.91.7.2488","article-title":"The zinc finger region of the adenovirus E1A transactivating domain complexes with the TATA box binding protein","volume":"91","author":"Geisberg","year":"1994","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"9186","DOI":"10.1128\/JVI.76.18.9186-9193.2002","article-title":"In vivo association of adenovirus large E1A protein with the human mediator complex in adenovirus-infected and -transformed cells","volume":"76","author":"Wang","year":"2002","journal-title":"J. Virol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"12771","DOI":"10.1128\/JVI.01243-10","article-title":"Comparison of E1A CR3-dependent transcriptional activation across six different human adenovirus subgroups","volume":"84","author":"Ablack","year":"2010","journal-title":"J. Virol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1016\/0092-8674(79)90333-7","article-title":"Pre-early adenovirus 5 gene product regulates synthesis of early viral messenger RNAs","volume":"17","author":"Berk","year":"1979","journal-title":"Cell"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"3665","DOI":"10.1073\/pnas.76.8.3665","article-title":"An adenovirus type 5 early gene function regulates expression of other early viral genes","volume":"76","author":"Jones","year":"1979","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"King, C.R., Gameiro, S.F., Tessier, T.M., Zhang, A., and Mymryk, J.S. (2018). Mimicry of Cellular A Kinase-Anchoring Proteins Is a Conserved and Critical Function of E1A across Various Human Adenovirus Species. J. Virol., 92.","DOI":"10.1128\/JVI.01902-17"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"10348","DOI":"10.1128\/JVI.00786-13","article-title":"Dissection of the C-terminal region of E1A redefines the roles of CtBP and other cellular targets in oncogenic transformation","volume":"87","author":"Cohen","year":"2013","journal-title":"J. Virol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1186\/s12964-015-0120-z","article-title":"Short linear motifs - ex nihilo evolution of protein regulation","volume":"13","author":"Davey","year":"2015","journal-title":"Cell Commun. Signal."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/10\/12\/662\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T15:31:16Z","timestamp":1760196676000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/10\/12\/662"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,11,22]]},"references-count":63,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2018,12]]}},"alternative-id":["v10120662"],"URL":"https:\/\/doi.org\/10.3390\/v10120662","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,11,22]]}}}