{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T02:56:36Z","timestamp":1790218596244,"version":"4.1.0"},"reference-count":37,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2018,12,19]],"date-time":"2018-12-19T00:00:00Z","timestamp":1545177600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["261307"],"award-info":[{"award-number":["261307"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Fondo de Apoyo a la Investigaci\u00f3n Hospital Infantil de M\u00e9xico Federico G\u00f3mez","award":["HIM-2016-021"],"award-info":[{"award-number":["HIM-2016-021"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Herpesviruses are common components of the human microbiome that become clinically relevant when a competent immunosurveillance is compromised, such as in transplantation. Members of the beta and gamma subfamilies are associated with a wide diversity of pathologies, including end-organ disease and cancer. In this study, we developed a multiplex qPCR technique with high specificity, sensitivity, efficiency and predictability that allowed the simultaneous detection and quantification of beta and gamma human herpesviruses. The technique was tested in a cohort of 34 kidney- or liver-transplanted pediatric patients followed up for up to 12 months post-transplant. Viral load was determined in 495 leukocyte-plasma paired samples collected bi-weekly or monthly. Human herpesvirus (HHV) 7 was the herpesvirus most frequently found in positive samples (39%), followed by Epstein-Barr virus (EBV) (20%). Also, EBV and HHV7 were present in the majority of coinfection episodes (62%). The share of positive samples exclusively detected either in leukocytes or plasma was 85%, suggesting that these herpesviruses tended to take a latent or lytic path in an exclusive manner. Infection by human cytomegalovirus (HCMV) and HHV6, as well as coinfection by EBV\/HHV7 and EBV\/HHV6\/HHV7, were associated with graft rejection (RR = 40.33 (p = 0.0013), 5.60 (p = 0.03), 5.60 (p = 0.03) and 17.64 (p = 0.0003), respectively). The routine monitoring of beta and gamma herpesviruses should be mandatory in transplant centers to implement preventive strategies.<\/jats:p>","DOI":"10.3390\/v10120730","type":"journal-article","created":{"date-parts":[[2018,12,19]],"date-time":"2018-12-19T12:12:44Z","timestamp":1545221564000},"page":"730","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Simultaneous Detection of Beta and Gamma Human Herpesviruses by Multiplex qPCR Reveals Simple Infection and Coinfection Episodes Increasing Risk for Graft Rejection in Solid Organ Transplantation"],"prefix":"10.3390","volume":"10","author":[{"given":"Yessica","family":"S\u00e1nchez-Ponce","sequence":"first","affiliation":[{"name":"Research Unit in Virology and Cancer, Children\u2019s Hospital of Mexico Federico G\u00f3mez, 06720 Mexico City, Mexico"},{"name":"Postgraduate Program in Biological Science, National Autonomous University of Mexico, 04510 Mexico City, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo","family":"Varela-Fascinetto","sequence":"additional","affiliation":[{"name":"Department of Transplantation, Children\u2019s Hospital of Mexico Federico G\u00f3mez, 06720 Mexico City, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Carlos","family":"Romo-V\u00e1zquez","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Children\u2019s Hospital of Mexico Federico G\u00f3mez, 06720 Mexico City, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Briceida","family":"L\u00f3pez-Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Subdirection of Diagnostic Auxiliary Services, Children\u2019s Hospital of Mexico Federico G\u00f3mez, 06720 Mexico City, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1451-9415","authenticated-orcid":false,"given":"Jos\u00e9 Luis","family":"S\u00e1nchez-Huerta","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Children\u2019s Hospital of Mexico Federico G\u00f3mez, 06720 Mexico City, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Israel","family":"Parra-Ortega","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Children\u2019s Hospital of Mexico Federico G\u00f3mez, 06720 Mexico City, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2872-0459","authenticated-orcid":false,"given":"Ezequiel M.","family":"Fuentes-Panan\u00e1","sequence":"additional","affiliation":[{"name":"Research Unit in Virology and Cancer, Children\u2019s Hospital of Mexico Federico G\u00f3mez, 06720 Mexico City, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2951-6716","authenticated-orcid":false,"given":"Abigail","family":"Morales-S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Research Unit in Virology and Cancer, Children\u2019s Hospital of Mexico Federico G\u00f3mez, 06720 Mexico City, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,12,19]]},"reference":[{"key":"ref_1","unstructured":"Fields, B.N., Knipe, D.M., and Howley, P.M. 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