{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T04:07:19Z","timestamp":1760242039771,"version":"build-2065373602"},"reference-count":15,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2018,12,28]],"date-time":"2018-12-28T00:00:00Z","timestamp":1545955200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["#2017\/00021-9 #2015\/12944-9 #2016\/01735-2"],"award-info":[{"award-number":["#2017\/00021-9 #2015\/12944-9 #2016\/01735-2"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Enterovirus B73 is a new member of the Enterovirus B species. First detected in the USA, it has been subsequently identified in China, India, Oman, and the Netherlands. In this study, we characterize the first B73 strain (named TO-127) to be detected in South America. TO-127 was obtained from a child with acute gastroenteritis living in a rural area in Northern Brazil. The subject was not infected with any known enteric pathogens such as norovirus, rotavirus, helminths, or enteric bacteria. Analysis of the nearly full-length TO-127 genome (6993 nt) indicated a 74\u201375% nucleotide similarity with EV-B73 strains from other countries. Evolutionary analysis suggests that B73 is endemic and widespread.<\/jats:p>","DOI":"10.3390\/v11010016","type":"journal-article","created":{"date-parts":[[2018,12,28]],"date-time":"2018-12-28T11:52:42Z","timestamp":1545997962000},"page":"16","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Detection and Characterization of Enterovirus B73 from a Child in Brazil"],"prefix":"10.3390","volume":"11","author":[{"given":"Geovani de Oliveira","family":"Ribeiro","sequence":"first","affiliation":[{"name":"Institute of Biological Sciences, Federal University of Par\u00e1, Par\u00e1 66075-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriana","family":"Luchs","sequence":"additional","affiliation":[{"name":"Enteric Disease Laboratory, Virology Center, Adolfo Lutz Institute, S\u00e3o Paulo 01246-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fl\u00e1vio Augusto de P\u00e1dua","family":"Milagres","sequence":"additional","affiliation":[{"name":"Secretary of Health of Tocantins, Tocantins 77453-000, Brazil"},{"name":"Institute of Biological Sciences, Federal University of Tocantins, Tocantins 77001-090, Brazil"},{"name":"Public Health Laboratory of Tocantins State (LACEN\/TO), Tocantins 77016-330, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shirley Vasconcelos","family":"Komninakis","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Health Science, Faculty of Medicine of ABC, Santo Andr\u00e9 09060-870, Brazil"},{"name":"Retrovirology Laboratory, Federal University of S\u00e3o Paulo, S\u00e3o Paulo 04023-062, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danielle Elise","family":"Gill","sequence":"additional","affiliation":[{"name":"Institute of Tropical Medicine, University of S\u00e3o Paulo, S\u00e3o Paulo 05403-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00e1rcia Cristina Alves Brito Say\u00e3o","family":"Lobato","sequence":"additional","affiliation":[{"name":"Public Health Laboratory of Tocantins State (LACEN\/TO), Tocantins 77016-330, Brazil"},{"name":"Postgraduate Program in Health Science, Faculty of Medicine of ABC, Santo Andr\u00e9 09060-870, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafael","family":"Brustulin","sequence":"additional","affiliation":[{"name":"Secretary of Health of Tocantins, Tocantins 77453-000, Brazil"},{"name":"Institute of Biological Sciences, Federal University of Tocantins, Tocantins 77001-090, Brazil"},{"name":"Public Health Laboratory of Tocantins State (LACEN\/TO), Tocantins 77016-330, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rog\u00e9rio Togisaki das","family":"Chagas","sequence":"additional","affiliation":[{"name":"Secretary of Health of Tocantins, Tocantins 77453-000, Brazil"},{"name":"Public Health Laboratory of Tocantins State (LACEN\/TO), Tocantins 77016-330, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria de F\u00e1tima Neves dos Santos","family":"Abr\u00e3o","sequence":"additional","affiliation":[{"name":"Secretary of Health of Tocantins, Tocantins 77453-000, Brazil"},{"name":"Public Health Laboratory of Tocantins State (LACEN\/TO), Tocantins 77016-330, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cassia Vit\u00f3ria de Deus Alves","family":"Soares","sequence":"additional","affiliation":[{"name":"Secretary of Health of Tocantins, Tocantins 77453-000, Brazil"},{"name":"Public Health Laboratory of Tocantins State (LACEN\/TO), Tocantins 77016-330, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven S.","family":"Witkin","sequence":"additional","affiliation":[{"name":"Institute of Tropical Medicine, University of S\u00e3o Paulo, S\u00e3o Paulo 05403-000, Brazil"},{"name":"Department of Obstetrics and Gynecology, Weill Cornell Medicine, New York, NY 10065, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabiola","family":"Villanova","sequence":"additional","affiliation":[{"name":"Institute of Biological Sciences, Federal University of Par\u00e1, Par\u00e1 66075-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xutao","family":"Deng","sequence":"additional","affiliation":[{"name":"Blood Systems Research Institute, San Francisco, CA 94143, USA"},{"name":"Department Laboratory Medicine, University of California San Francisco, San Francisco, CA 94143, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ester Cerdeira","family":"Sabino","sequence":"additional","affiliation":[{"name":"Institute of Tropical Medicine, University of S\u00e3o Paulo, S\u00e3o Paulo 05403-000, Brazil"},{"name":"LIM\/46, Faculty of Medicine, University of S\u00e3o Paulo, S\u00e3o Paulo 01246-903, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6296-4484","authenticated-orcid":false,"given":"Eric","family":"Delwart","sequence":"additional","affiliation":[{"name":"Blood Systems Research Institute, San Francisco, CA 94143, USA"},{"name":"Department Laboratory Medicine, University of California San Francisco, San Francisco, CA 94143, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5516-5177","authenticated-orcid":false,"given":"Antonio Charlys","family":"Da Costa","sequence":"additional","affiliation":[{"name":"Institute of Tropical Medicine, University of S\u00e3o Paulo, S\u00e3o Paulo 05403-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3463-0492","authenticated-orcid":false,"given":"\u00c9lcio","family":"Leal","sequence":"additional","affiliation":[{"name":"Institute of Biological Sciences, Federal University of Par\u00e1, Par\u00e1 66075-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2018,12,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2421","DOI":"10.1099\/jgv.0.000911","article-title":"ICTV virus taxonomy profile: Picornaviridae","volume":"98","author":"Zell","year":"2017","journal-title":"J. Gen. Virol."},{"key":"ref_2","unstructured":"Knipe, D.M., and Howley, P.M. (2013). Enteroviruses: Polioviruses, Coxsackieviruses, Echoviruses, and Newer Enteroviruses. Fields Virology, Lippincott Williams & Wilkins."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1099\/0022-1317-82-2-409","article-title":"Molecular identification of new picornaviruses and characterization of a proposed enterovirus 73 serotype","volume":"82","author":"Oberste","year":"2001","journal-title":"J. Gen. Virol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1742","DOI":"10.3201\/eid2310.170307","article-title":"Spread of Chikungunya Virus East\/Central\/South African Genotype in Northeast Brazil","volume":"23","author":"Komninakis","year":"2017","journal-title":"Emerg. Infect. Dis."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"e46","DOI":"10.1093\/nar\/gkv002","article-title":"An ensemble strategy that significantly improves de novo assembly of microbial genomes from metagenomic next-generation sequencing data","volume":"43","author":"Deng","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.jcv.2011.03.006","article-title":"An automated genotyping tool for enteroviruses and noroviruses","volume":"51","author":"Kroneman","year":"2011","journal-title":"J. Clin. Virol."},{"key":"ref_7","first-page":"95","article-title":"BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95\/98\/NT","volume":"41","author":"Hall","year":"1999","journal-title":"Nucleic Acids Symp. Ser."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1093\/bib\/bbn013","article-title":"Recent developments in the MAFFT multiple sequence alignment program","volume":"9","author":"Katoh","year":"2008","journal-title":"Brief. Bioinform."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1721","DOI":"10.1099\/0022-1317-83-7-1721","article-title":"Open reading frame sequence of an Asian enterovirus 73 strain reveals that the prototype from California is recombinant","volume":"83","author":"Norder","year":"2002","journal-title":"J. Gen. Virol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1941","DOI":"10.1128\/JVI.73.3.1941-1948.1999","article-title":"Molecular evolution of the human enteroviruses: Correlation of serotype with VP1 sequence and application to picornavirus classification","volume":"73","author":"Oberste","year":"1999","journal-title":"J. Virol."},{"key":"ref_12","first-page":"185","article-title":"Status of enterovirus infection and molecular identification among healthy children at the areas bordering Myanmar, in Yunnan province, China","volume":"31","author":"Ding","year":"2010","journal-title":"Zhonghua Liu Xing Bing Xue Za Zhi"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"3808","DOI":"10.1038\/s41598-017-03835-1","article-title":"Identification and molecular characterization of non-polio enteroviruses from children with acute flaccid paralysis in West Africa, 2013\u20132014","volume":"7","author":"Kebe","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"6058","DOI":"10.1038\/srep06058","article-title":"Molecular epidemiology and recombination of human enteroviruses from AFP surveillance in Yunnan, China from 2006 to 2010","volume":"4","author":"Tang","year":"2014","journal-title":"Sci. Rep."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1007\/s11262-014-1077-5","article-title":"Complete genome analysis of human enterovirus B73 isolated from an acute flaccid paralysis patient in Shandong, China","volume":"49","author":"Liu","year":"2014","journal-title":"Virus Genes"}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/11\/1\/16\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T15:36:23Z","timestamp":1760196983000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/11\/1\/16"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,12,28]]},"references-count":15,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2019,1]]}},"alternative-id":["v11010016"],"URL":"https:\/\/doi.org\/10.3390\/v11010016","relation":{},"ISSN":["1999-4915"],"issn-type":[{"type":"electronic","value":"1999-4915"}],"subject":[],"published":{"date-parts":[[2018,12,28]]}}}