{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T03:31:06Z","timestamp":1760239866576,"version":"build-2065373602"},"reference-count":38,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2019,1,25]],"date-time":"2019-01-25T00:00:00Z","timestamp":1548374400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Italian Ministry of Health","award":["IZS LT 03\/13 RC"],"award-info":[{"award-number":["IZS LT 03\/13 RC"]}]},{"DOI":"10.13039\/501100000780","name":"European Union","doi-asserted-by":"publisher","award":["653316","677066"],"award-info":[{"award-number":["653316","677066"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Orthopoxviruses (OPV) are emerging zoonotic pathogens, and an increasing number of human infections is currently reported in Europe and in other continents, warranting heightened attention on this topic. Following two OPV infections reported in veterinarians scratched by sick cats in 2005 and 2007 in North-Eastern-Italy, involving a previously undescribed OPV, a similar strain was isolated by a sick cat from the same territory in 2011, i.e., 6 years later, raising attention on OPV circulation in this region. A surveillance program was launched to assess the OPV seroprevalence among the veterinarians working in local veterinary clinics and in the local wild and domestic cat population; seroprevalence was 33.3% in veterinarians and 19.5% in cats. Seroprevalence in cats was unevenly distributed, peaking at 40% in the area where OPV-infected cats had been observed.<\/jats:p>","DOI":"10.3390\/v11020101","type":"journal-article","created":{"date-parts":[[2019,1,25]],"date-time":"2019-01-25T11:30:00Z","timestamp":1548415800000},"page":"101","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Orthopoxvirus Seroprevalence in Cats and Veterinary Personnel in North-Eastern Italy in 2011"],"prefix":"10.3390","volume":"11","author":[{"given":"Daniele","family":"Lapa","sequence":"first","affiliation":[{"name":"National Institute for Infectious Diseases Lazzaro Spallanzani, 00149 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Beltrame","sequence":"additional","affiliation":[{"name":"Centre for Tropical Diseases, IRCCS Sacro Cuore Don Calabria Hospital, 37024 Negrar VR, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandra","family":"Arzese","sequence":"additional","affiliation":[{"name":"Medical Department (DAME), University of Udine, 33100 Udine, Italy"},{"name":"Microbiology Laboratory Unit, Azienda Sanitaria Universitaria Integrata, Santa Maria della Misericordia University Hospital, 33100 Udine, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1952-5117","authenticated-orcid":false,"given":"Fabrizio","family":"Carletti","sequence":"additional","affiliation":[{"name":"National Institute for Infectious Diseases Lazzaro Spallanzani, 00149 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6027-3009","authenticated-orcid":false,"given":"Antonino","family":"Di Caro","sequence":"additional","affiliation":[{"name":"National Institute for Infectious Diseases Lazzaro Spallanzani, 00149 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1076-2979","authenticated-orcid":false,"given":"Giuseppe","family":"Ippolito","sequence":"additional","affiliation":[{"name":"National Institute for Infectious Diseases Lazzaro Spallanzani, 00149 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Rosaria","family":"Capobianchi","sequence":"additional","affiliation":[{"name":"National Institute for Infectious Diseases Lazzaro Spallanzani, 00149 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9819-236X","authenticated-orcid":false,"given":"Concetta","family":"Castilletti","sequence":"additional","affiliation":[{"name":"National Institute for Infectious Diseases Lazzaro Spallanzani, 00149 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,1,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.vetmic.2009.08.026","article-title":"Zoonotic poxviruses","volume":"140","author":"Essbauer","year":"2010","journal-title":"Vet. Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1111\/zph.12459","article-title":"Occupational transmission of an Orthopoxvirus infection during an outbreak in a colony of Macaca tonkeana in Lazio Region, Italy, 2015","volume":"65","author":"Puro","year":"2018","journal-title":"Zoonoses Public Health"},{"unstructured":"World Health Organization (WHO) (2018, November 12). Emergencies Preparedness, Response Monkeypox\u2014Nigeria. Available online: http:\/\/www.who.int\/csr\/don\/05-october-2018-monkeypox-nigeria\/en\/.","key":"ref_3"},{"unstructured":"Public Health England (2018, November 12). Monkeypox Case in England, Available online: https:\/\/www.gov.uk\/government\/news\/monkeypox-case-in-england.","key":"ref_4"},{"unstructured":"Center for Infectious Disease Research and Policy (CIDRAP) (2018, November 12). News Scan for Oct 12, 2018. Israel Confirms First Imported Monkeypox Case. Available online: http:\/\/www.cidrap.umn.edu\/news-perspective\/2018\/10\/news-scan-oct-12-2018.","key":"ref_5"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1016\/S1473-3099(10)70054-2","article-title":"Human cowpox in a veterinary student","volume":"10","author":"Glatz","year":"2010","journal-title":"Lancet Infect. Dis."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1133","DOI":"10.1089\/vbz.2010.0170","article-title":"Orthopox virus infections in Eurasian wild rodents","volume":"11","author":"Kinnunen","year":"2011","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1097\/QCO.0b013e3282f44c74","article-title":"Cowpox virus infection: An emerging health threat","volume":"21","author":"Vorou","year":"2008","journal-title":"Curr. Opin. Infect. Dis."},{"doi-asserted-by":"crossref","unstructured":"Duraffour, S., Mertens, B., Meyer, H., van den Oord, J.J., Mitera, T., Matthys, P., Snoeck, R., and Andrei, G. (2013). Emergence of cowpox: Study of the virulence of clinical strains and evaluation of antivirals. PLoS ONE, 8.","key":"ref_9","DOI":"10.1371\/journal.pone.0055808"},{"doi-asserted-by":"crossref","unstructured":"Shchelkunov, S.N. (2013). An increasing danger of zoonotic orthopoxvirus infections. PLoS Pathog., 9.","key":"ref_10","DOI":"10.1371\/journal.ppat.1003756"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1111\/j.1365-3164.2010.00923.x","article-title":"Localized cowpox infection in a 5-month-old Rottweiler","volume":"22","author":"Mauldin","year":"2011","journal-title":"Vet. Dermatol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"781","DOI":"10.3201\/eid1505.090235","article-title":"Cowpox virus transmission from pet rats to humans, France","volume":"15","author":"Ninove","year":"2009","journal-title":"Emerg. Infect. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"670","DOI":"10.3201\/eid1404.070817","article-title":"Rat-to-elephant-to-human transmission of cowpox virus","volume":"14","author":"Kurth","year":"2008","journal-title":"Emerg. Infect. Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"777","DOI":"10.3201\/eid1505.090159","article-title":"Cowpox virus transmission from pet rats to humans, Germany","volume":"15","author":"Campe","year":"2009","journal-title":"Emerg. Infect. Dis."},{"key":"ref_15","first-page":"329","article-title":"Cowpox virus infection in pet rat owners: Not always immediately recognized","volume":"106","author":"Becker","year":"2009","journal-title":"Dtsch. Arztebl. Int."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"126","DOI":"10.2340\/00015555-1227","article-title":"The Munich outbreak of cutaneous cowpox infection: Transmission by infected pet rats","volume":"92","author":"Vogel","year":"2012","journal-title":"Acta Derm. Venereol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"377","DOI":"10.3390\/ani1040377","article-title":"Zoonotic Poxviruses Associated with Companion Animals","volume":"1","author":"Tack","year":"2011","journal-title":"Animals"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1017\/S0950268899002423","article-title":"Cowpox: Reservoir hosts and geographic range","volume":"122","author":"Chantrey","year":"1999","journal-title":"Epidemiol. Infect."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1007\/BF01313805","article-title":"Studies on poxvirus infection in cats","volume":"104","author":"Bennett","year":"1989","journal-title":"Arch. Virol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"672","DOI":"10.7589\/0090-3558-42.3.672","article-title":"Serological survey for viral pathogens in Turkish rodents","volume":"42","author":"Laakkonen","year":"2006","journal-title":"J. Wild Dis."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1089\/vbz.2008.0205","article-title":"Patterns of orthopox virus wild rodent hosts in South Germany","volume":"9","author":"Essbauer","year":"2009","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1089\/vbz.2014.1731","article-title":"Serologic survey of orthopoxvirus infection among rodents in hungary","volume":"15","author":"Oldal","year":"2015","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1136\/vr.103.25.567-b","article-title":"Cowpox in the domestic cat","volume":"103","author":"Thomsett","year":"1978","journal-title":"Vet. Rec."},{"key":"ref_24","first-page":"55","article-title":"Feline cowpoxvirus infections in Germany: Clinical and epidemiological aspects","volume":"126","author":"Appl","year":"2013","journal-title":"Berl. Munch. Tierarztl. Wochenschr."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"499","DOI":"10.3201\/eid1503.080813","article-title":"Cat-to-human orthopoxvirus transmission, northeastern Italy","volume":"15","author":"Carletti","year":"2009","journal-title":"Emerg. Infect. Dis."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1665","DOI":"10.3201\/eid2409.171283","article-title":"Novel Orthopoxvirus and Lethal Disease in Cat, Italy","volume":"24","author":"Lanave","year":"2018","journal-title":"Emerg. Infect. Dis."},{"doi-asserted-by":"crossref","unstructured":"Franke, A., Pfaff, F., Jenckel, M., Hoffmann, B., H\u00f6per, D., Antwerpen, M., Meyer, H., Beer, M., and Hoffmann, D. (2017). Classification of Cowpox Viruses into Several Distinct Clades and Identification of a Novel Lineage. Viruses, 9.","key":"ref_27","DOI":"10.3390\/v9060142"},{"doi-asserted-by":"crossref","unstructured":"Mauldin, M.R., Antwerpen, M., Emerson, G.L., Li, Y., Zoeller, G., Carroll, D.S., and Meyer, H. (2017). Cowpox virus: What\u2019s in a Name?. Viruses, 9.","key":"ref_28","DOI":"10.3390\/v9050101"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1136\/vr.150.2.50","article-title":"Retrospective investigation of feline cowpox in Germany","volume":"150","author":"Pfeffer","year":"2002","journal-title":"Vet. Rec."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/S0378-1135(96)00072-7","article-title":"A monoclonal blocking-ELISA for detection of orthopoxvirus antibodies in feline sera","volume":"52","author":"Czerny","year":"1996","journal-title":"Vet. Microbiol."},{"key":"ref_31","first-page":"452","article-title":"Serologic studies of domestic cats for potential human pathogenic virus infections from wild rodens [in German]","volume":"198","author":"Nowotny","year":"1996","journal-title":"Zentralbl. Hyg. Umweltmed."},{"key":"ref_32","first-page":"423","article-title":"Poxvirus infections in a cat and its owner","volume":"19","author":"Zimmer","year":"1991","journal-title":"Tierarztl. Prax."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1136\/vr.143.4.105","article-title":"Antibodies to orthopoxvirus in domestic cats in Norway","volume":"143","author":"Tryland","year":"1998","journal-title":"Vet. Rec."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1941","DOI":"10.3201\/eid2312.162098","article-title":"Fatal Outbreak in Tonkean Macaques Caused by Possibly Novel Orthopoxvirus, Italy","volume":"23","author":"Cardeti","year":"2017","journal-title":"Emerg. Infect. Dis."},{"doi-asserted-by":"crossref","unstructured":"Gruber, C.E.M., Giombini, E., Selleri, M., Tausch, S.H., Andrusch, A., Tyshaieva, A., Cardeti, G., Lorenzetti, R., De Marco, L., and Carletti, F. (2018). Whole Genome Characterization of Orthopoxvirus (OPV) Abatino, a Zoonotic Virus Representing a Putative Novel Clade of Old World Orthopoxviruses. Viruses, 10.","key":"ref_35","DOI":"10.3390\/v10100546"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.jcv.2016.10.002","article-title":"Lymphocytic choriomeningitis, Ljungan and orthopoxvirus seroconversions in patients hospitalized due to acute Puumala hantavirus infection","volume":"84","author":"Fevola","year":"2016","journal-title":"J. Clin. Virol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"275","DOI":"10.4049\/jimmunol.85.3.275","article-title":"The antibody response to smallpox vaccination as measured by tissue-culture plaque method","volume":"85","author":"Cutchins","year":"1960","journal-title":"J. Immunol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"3154","DOI":"10.1128\/JCM.41.7.3154-3157.2003","article-title":"Improved assay to detect neutralizing antibody following vaccination with diluted or undiluted vaccinia (Dryvax) vaccine","volume":"41","author":"Newman","year":"2003","journal-title":"J. Clin. Microbiol."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/11\/2\/101\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T12:28:40Z","timestamp":1760185720000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/11\/2\/101"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1,25]]},"references-count":38,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2019,2]]}},"alternative-id":["v11020101"],"URL":"https:\/\/doi.org\/10.3390\/v11020101","relation":{},"ISSN":["1999-4915"],"issn-type":[{"type":"electronic","value":"1999-4915"}],"subject":[],"published":{"date-parts":[[2019,1,25]]}}}