{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,11]],"date-time":"2026-11-11T20:44:54Z","timestamp":1794429894368,"version":"4.9.1"},"reference-count":6,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2019,2,19]],"date-time":"2019-02-19T00:00:00Z","timestamp":1550534400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Counting labeled cells, after immunofluorescence or expression of a genetically fluorescent reporter protein, is frequently used to quantify viral infection. However, this can be very tedious without a high content screening apparatus. For this reason, we have developed QuantIF, an ImageJ macro that automatically determines the total number of cells and the number of labeled cells from two images of the same field, using DAPI- and specific-stainings, respectively. QuantIF can automatically analyze hundreds of images, taking approximately one second for each field. It is freely available as supplementary data online at MDPI.com and has been developed using ImageJ, a free image processing program that can run on any computer with a Java virtual machine, which is distributed for Windows, Mac, and Linux. It is routinely used in our labs to quantify viral infections in vitro, but can easily be used for other applications that require quantification of labeled cells.<\/jats:p>","DOI":"10.3390\/v11020165","type":"journal-article","created":{"date-parts":[[2019,2,20]],"date-time":"2019-02-20T03:05:52Z","timestamp":1550631952000},"page":"165","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["QuantIF: An ImageJ Macro to Automatically Determine the Percentage of Infected Cells after Immunofluorescence"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5033-4357","authenticated-orcid":false,"given":"Lynda","family":"Handala","sequence":"first","affiliation":[{"name":"EA4294, Agents Infectieux, R\u00e9sistance et Chimioth\u00e9rapie, Centre Universitaire de Recherche en Sant\u00e9, Centre Hospitalier Universitaire et Universit\u00e9 de Picardie Jules Verne, 80054 Amiens, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2209-0660","authenticated-orcid":false,"given":"Tony","family":"Fiore","sequence":"additional","affiliation":[{"name":"EA4294, Agents Infectieux, R\u00e9sistance et Chimioth\u00e9rapie, Centre Universitaire de Recherche en Sant\u00e9, Centre Hospitalier Universitaire et Universit\u00e9 de Picardie Jules Verne, 80054 Amiens, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0788-9271","authenticated-orcid":false,"given":"Yves","family":"Rouill\u00e9","sequence":"additional","affiliation":[{"name":"University of Lille, CNRS, INSERM, CHU Lille, Pasteur Institute of Lille, U1019-UMR8204-CIIL-Center for Infection and Immunity of Lille, 59019 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6884-2456","authenticated-orcid":false,"given":"Francois","family":"Helle","sequence":"additional","affiliation":[{"name":"EA4294, Agents Infectieux, R\u00e9sistance et Chimioth\u00e9rapie, Centre Universitaire de Recherche en Sant\u00e9, Centre Hospitalier Universitaire et Universit\u00e9 de Picardie Jules Verne, 80054 Amiens, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,2,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1002\/mrd.22489","article-title":"The ImageJ ecosystem: An open platform for biomedical image analysis","volume":"82","author":"Schindelin","year":"2015","journal-title":"Mol. Reprod. Dev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH Image to ImageJ: 25 years of image analysis","volume":"9","author":"Schneider","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/0031-3203(94)E0043-K","article-title":"Image thresholding by minimizing the measures of fuzziness","volume":"28","author":"Huang","year":"1995","journal-title":"Pattern Recognit."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1109\/34.87344","article-title":"Watersheds in digital spaces: An efficient algorithm based on immersion simulations","volume":"13","author":"Vincent","year":"1991","journal-title":"IEEE Trans. Pattern Anal. Mach. Intell."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1086","DOI":"10.1099\/jgv.0.001099","article-title":"Investigation of the role of GBF1 in the replication of positive-sense single-stranded RNA viruses","volume":"99","author":"Ferlin","year":"2018","journal-title":"J. Gen. Virol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2832","DOI":"10.1128\/JVI.80.6.2832-2841.2006","article-title":"Subcellular localization of hepatitis C virus structural proteins in a cell culture system that efficiently replicates the virus","volume":"80","author":"Rouille","year":"2006","journal-title":"J. Virol."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/11\/2\/165\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T12:33:10Z","timestamp":1760185990000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/11\/2\/165"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,2,19]]},"references-count":6,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2019,2]]}},"alternative-id":["v11020165"],"URL":"https:\/\/doi.org\/10.3390\/v11020165","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,2,19]]}}}