{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T01:50:23Z","timestamp":1784253023167,"version":"3.55.0"},"reference-count":44,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2019,5,24]],"date-time":"2019-05-24T00:00:00Z","timestamp":1558656000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008648","name":"Krajowy Naukowy Osrodek Wiodacy","doi-asserted-by":"publisher","award":["KNOW2015\/CB\/PRO1\/39"],"award-info":[{"award-number":["KNOW2015\/CB\/PRO1\/39"]}],"id":[{"id":"10.13039\/501100008648","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Porcine parvovirus (PPV) is a major causative agent in reproductive failure, but in the last two decades many novel porcine parvoviruses were described and designated as porcine parvovirus 2 through 6 (PPV2\u2013PPV6). However, their role for pig health is largely unknown. The aim of this study was to better understand the on-farm prevalence of PPVs in different age groups of pigs, and to assess the diagnostic applicability of testing different diagnostic materials. In total, 271 oral fluids, 1244 serum samples, and 1238 fecal samples were collected from 3\u201321-week-old pigs from 19 farms, and after pooling by 4\u20136, tested by real-time PCR. The results showed that PPVs are widely spread in Poland and that the highest detection rates were obtained for oral fluids (ranging from 10.7% (PPV1) to 48.7% (PPV2)). Fattening pigs were the age group with the most frequent detection of PPVs (ranging from 8.6% (PPV1) to 49.1% (PPV2)). Porcine parvoviruses were detected mostly in growing-finishing pigs and the infection persisted until the late fattening period, which may suggest the chronic character of the infection (especially for PPV2, which was found to commonly infect animals of all ages). Particularly low Ct values detected for PPV2, PPV3, PPV5, and PPV6 in serum pools from some farms suggested that these viruses may cause high levels of viremia in one or more individuals included in these pools. Further studies are needed to quantify the levels of PPVs viremia and to assess the impact in co-infections with other, often endemic pig viruses, such as porcine circovirus type 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV).<\/jats:p>","DOI":"10.3390\/v11050474","type":"journal-article","created":{"date-parts":[[2019,5,24]],"date-time":"2019-05-24T11:20:46Z","timestamp":1558696846000},"page":"474","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":44,"title":["Detection Patterns of Porcine Parvovirus (PPV) and Novel Porcine Parvoviruses 2 through 6 (PPV2\u2013PPV6) in Polish Swine Farms"],"prefix":"10.3390","volume":"11","author":[{"given":"Dagmara","family":"Mi\u0142ek","sequence":"first","affiliation":[{"name":"Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Nowoursynowska 159C, 02-776 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleksandra","family":"Wo\u017aniak","sequence":"additional","affiliation":[{"name":"Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Nowoursynowska 159C, 02-776 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalena","family":"Guzowska","sequence":"additional","affiliation":[{"name":"Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8889-8120","authenticated-orcid":false,"given":"Tomasz","family":"Stadejek","sequence":"additional","affiliation":[{"name":"Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Nowoursynowska 159C, 02-776 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,5,24]]},"reference":[{"key":"ref_1","unstructured":"King, A.M.Q., Adams, M.J., Carstens, E.B., and Lefkowitz, E.J. (2012). Family Parvoviridae. Virus Taxonomy. 9th report of the International Committee on Taxonomy of Viruses, Academic Press."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1177\/104063879100300107","article-title":"A current assessment of the role of porcine parvovirus as a cause of fetal porcine death","volume":"3","author":"Mengeling","year":"1991","journal-title":"J. Vet. Diagn. Investig."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/S0378-4320(00)00135-4","article-title":"The effect of porcine parvovirus and porcine reproductive and respiratory syndrome virus on porcine reproductive performance","volume":"60","author":"Mengeling","year":"2000","journal-title":"Anim. Reprod. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1053\/jcpa.1998.0295","article-title":"Experimental reproduction of severe wasting disease by co-infection of pigs with porcine circovirus and porcine parvovirus","volume":"121","author":"Allan","year":"1999","journal-title":"J. Comp. Pathol."},{"key":"ref_5","first-page":"244","article-title":"Identification of new parvovirus DNA sequence in swine sera from Myanmar","volume":"54","author":"Hijikata","year":"2001","journal-title":"Jpn. J. Infect. Dis."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1007\/s11262-010-0506-3","article-title":"Novel parvovirus sublineage in the family of Parvoviridae","volume":"41","author":"Wang","year":"2010","journal-title":"Virus Genes"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1840","DOI":"10.1099\/vir.0.2008\/000380-0","article-title":"Identification of novel porcine and bovine parvoviruses closely related to human parvovirus 4","volume":"89","author":"Lau","year":"2008","journal-title":"J. Gen. Virol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1007\/s00705-010-0646-8","article-title":"Identification and molecular cloning of a novel porcine parvovirus","volume":"155","author":"Cheung","year":"2010","journal-title":"Arch. Virol."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Xiao, C.T., Gim\u00e9nez-Lirola, L.G., Jiang, Y.H., Halbur, P.G., and Opriessnig, T. (2013). Characterization of a novel porcine parvovirus tentatively designated PPV5. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0065312"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1186\/s12985-014-0203-2","article-title":"Identification and genomic characterization of a novel porcine parvovirus (PPV6) in China","volume":"11","author":"Ni","year":"2014","journal-title":"Virol. J."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1007\/s11262-016-1322-1","article-title":"Discovery of a novel Parvovirinae virus, porcine parvovirus 7, by metagenomic sequencing of porcine rectal swabs","volume":"52","author":"Palinski","year":"2016","journal-title":"Virus Genes"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1099\/jgv.0.001212","article-title":"ICTV Virus Taxonomy Profile: Parvoviridae","volume":"100","author":"Cotmore","year":"2019","journal-title":"J. Gen. Virol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.1007\/s00705-015-2373-7","article-title":"Prevalence of emerging porcine parvoviruses and their co-infections with porcine circovirus type 2 in China","volume":"160","author":"Sun","year":"2015","journal-title":"Arch. Virol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1533","DOI":"10.1007\/s00705-013-1948-4","article-title":"First complete genomic characterization of a porcine parvovirus 5 isolate from China","volume":"159","author":"Wu","year":"2014","journal-title":"Arch. Virol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1007\/s00705-017-3585-9","article-title":"First identification of porcine parvovirus 7 in China","volume":"163","author":"Xing","year":"2017","journal-title":"Arch. Virol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.vetmic.2014.06.024","article-title":"Identification of recently described porcine parvoviruses in archived North American samples from 1996 and association with porcine circovirus associated disease","volume":"173","author":"Opriessnig","year":"2014","journal-title":"Vet. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1186\/s12985-015-0401-6","article-title":"First identification of porcine parvovirus 6 in North America by viral metagenomic sequencing of serum from pigs infected with porcine reproductive and respiratory syndrome virus","volume":"12","author":"Schirtzinger","year":"2015","journal-title":"Virol. J."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2330","DOI":"10.1099\/vir.0.055129-0","article-title":"Emerging novel porcine parvoviruses in Europe: Origin, evolution, phylodynamics and phylogeography","volume":"94","author":"Cadar","year":"2013","journal-title":"J. Gen. Virol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1945","DOI":"10.1111\/tbed.12593","article-title":"Circulation of Porcine Parvovirus Types 1 through 6 in Serum Samples Obtained from Six Commercial Polish Pig Farms","volume":"64","author":"Cui","year":"2017","journal-title":"Transbound Emerg. Dis."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.rvsc.2018.08.004","article-title":"The detection and genetic diversity of novel porcine parvovirus 7 (PPV7) on Polish pig farms","volume":"120","author":"Stadejek","year":"2018","journal-title":"Res. Vet. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1007\/s00705-012-1257-3","article-title":"Detection, prevalence and analysis of emerging porcine parvovirus infections","volume":"157","author":"Lorincz","year":"2012","journal-title":"Arch. Virol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1111\/1348-0421.12218","article-title":"Prevalence and genomic characterization of porcine parvoviruses detected in Chiangmai area of Thailand in 2011","volume":"59","author":"Saekhow","year":"2015","journal-title":"Microbiol. Immunol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1581","DOI":"10.1292\/jvms.15-0167","article-title":"Coincidental detection of genomes of porcine parvoviruses and porcine circovirus type 2 infecting pigs in Japan","volume":"77","author":"Saekhow","year":"2016","journal-title":"J. Vet. Med. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.actatropica.2018.10.010","article-title":"Prevalence of porcine parvoviruses in some South African swine herds with background of porcine circovirus type 2 infection","volume":"190","author":"Afolabi","year":"2018","journal-title":"Acta Trop."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"561","DOI":"10.3201\/eid1603.090746","article-title":"Parvovirus 4-like virus in blood products","volume":"16","author":"Szelei","year":"2010","journal-title":"Emerg. Infect. Dis."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.jviromet.2015.03.011","article-title":"A TaqMan-based real-time PCR for detection ad quantification of porcine parvovirus 4","volume":"19","author":"Gava","year":"2015","journal-title":"J. Virol. Methods"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"855","DOI":"10.4142\/jvs.2018.19.6.855","article-title":"First detection and genetic characterization of porcine parvovirus 7 from Korean domestic pig farms","volume":"19","author":"Ouh","year":"2018","journal-title":"J. Vet. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.vetmic.2012.12.019","article-title":"Emergence of a novel mutant PCV2b variant associated with clinical PCVAD in two vaccinated pig farms in the U.S. concurrently infected with PPV2","volume":"163","author":"Opriessnig","year":"2013","journal-title":"Vet. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Novosel, D., Cadar, D., Tuboly, T., Jungic, A., Stadejek, T., Ait-Ali, T., and Cs\u00e1gola, A. (2018). Investigating porcine parvoviruses genogroup 2 infection using in situ polymerase chain reaction. BMC Vet. Res., 14.","DOI":"10.1186\/s12917-018-1487-z"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1186\/1743-422X-7-333","article-title":"Detection of a novel porcine parvovirus, PPV4, in Chinese swine herds","volume":"7","author":"Huang","year":"2010","journal-title":"Virol. J."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"945","DOI":"10.1007\/s11250-017-1279-7","article-title":"Ungulate copiparvovirus 2 in healthy and postweaning multisystemic wasting syndrome-affected pigs","volume":"49","author":"Cibulski","year":"2017","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1016\/j.vetmic.2012.05.038","article-title":"Increasing porcine PARV4 prevalence with pig age in the U.S. pig population","volume":"160","author":"Xiao","year":"2012","journal-title":"Vet. Microbiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.vetmic.2012.07.038","article-title":"Characterization of porcine parvovirus type 2 (PPV2) which is highly prevalent in the USA","volume":"161","author":"Xiao","year":"2013","journal-title":"Vet. Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Cs\u00e1gola, A., Z\u00e1dori, Z., M\u00e9sz\u00e1ros, I., and Tuboly, T. (2016). Detection of Porcine Parvovirus 2 (Ungulate Tetraparvovirus 3) Specific Antibodies and Examination of the Serological Profile of an Infected Swine Herd. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0151036"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1261","DOI":"10.1128\/CVI.05057-11","article-title":"A live-attenuated chimeric porcine circovirus type 2 (PCV2) vaccine is transmitted to contact pigs but is not upregulated by concurrent infection with porcine parvovirus (PPV) and porcine reproductive and respiratory syndrome virus (PRRSV) and is efficacious in a PCV2b-PRRSV-PPV challenge model","volume":"18","author":"Opriessnig","year":"2011","journal-title":"Clin. Vaccine Immunol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1007\/s11262-016-1386-y","article-title":"First identification of porcine parvovirus 6 in Poland","volume":"53","author":"Cui","year":"2017","journal-title":"Virus Genes"},{"key":"ref_37","unstructured":"R Core Team (2019, May 17). R: A Language and Environment for Statistical Computing. Available online: https:\/\/www\/R-project.org\/."},{"key":"ref_38","unstructured":"Harrell, F.E. (2019, May 17). rms: Regression Modeling Strategies. R package version 5.1-3. Available online: https:\/\/CRAN.R-project.org\/package=rms."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1017\/S1466252310000010","article-title":"The development of oral fluid-based diagnostics and applications in veterinary medicine","volume":"11","author":"Prickett","year":"2010","journal-title":"Anim. Health Res. Rev."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"M\u00e9sz\u00e1ros, I., Olasz, F., Cs\u00e1gola, A., Tijssen, P., and Z\u00e1dori, Z. (2017). Biology of Porcine Parvovirus (Ungulate parvovirus 1). Viruses, 9.","DOI":"10.3390\/v9120393"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/BF01317841","article-title":"Observations on the pathogenesis of porcine parvovirus infection","volume":"51","author":"Joo","year":"1976","journal-title":"Arch. Virol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1007\/s11250-017-1301-0","article-title":"Dynamics of vanishing of maternally derived antibodies of Ungulate protoparvovirus 1 suggests an optimal age for gilts vaccination","volume":"49","author":"Gava","year":"2017","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_43","first-page":"237","article-title":"Transitional antibodies and spontaneous infection in the Transitional antibodies and spontaneous infection in the porcine (Sus scrofa) parvo viral infections","volume":"16","author":"Etoh","year":"1979","journal-title":"Jpn. J. Swine Husb. Res. (Japan)"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1408","DOI":"10.1099\/jgv.0.000446","article-title":"An inactivated whole-virus porcine parvovirus vaccine protects pigs against disease but does not prevent virus shedding even after homologous virus challenge","volume":"97","author":"Foerster","year":"2016","journal-title":"J. Gen. Virol."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/11\/5\/474\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T12:54:45Z","timestamp":1760187285000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/11\/5\/474"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,5,24]]},"references-count":44,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2019,5]]}},"alternative-id":["v11050474"],"URL":"https:\/\/doi.org\/10.3390\/v11050474","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,5,24]]}}}