{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T08:30:47Z","timestamp":1778661047525,"version":"3.51.4"},"reference-count":40,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2019,6,6]],"date-time":"2019-06-06T00:00:00Z","timestamp":1559779200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003196","name":"Ministero della Salute","doi-asserted-by":"publisher","award":["WFR GR-2011-023505919"],"award-info":[{"award-number":["WFR GR-2011-023505919"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Adenoviruses are important pathogens of humans and animals. Bats have been recognized as potential reservoirs of novel viruses, with some viruses being regarded as a possible zoonotic threat to humans. In this study, we report the detection and analysis of adenoviruses from different bat species in northern Italy. Upon sequence and phylogenetic analysis, based on a short diagnostic fragment of the highly-conserved DNA polymerase gene, we identified potential novel candidate adenovirus species, including an avian-like adenovirus strain. An adenovirus isolate was obtained in simian cell lines from the carcass of a Pipistrellus kuhlii, and the complete genome sequence was reconstructed using deep sequencing technologies. The virus displayed high nucleotide identity and virtually the same genome organization as the Pipistrellus pipistrellus strain PPV1, isolated in Germany in 2007. Gathering data on epidemiology and the genetic diversity of bat adenoviruses may be helpful to better understand their evolution in the mammalian and avian hosts.<\/jats:p>","DOI":"10.3390\/v11060523","type":"journal-article","created":{"date-parts":[[2019,6,7]],"date-time":"2019-06-07T03:56:31Z","timestamp":1559879791000},"page":"523","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Surveillance for Adenoviruses in Bats in Italy"],"prefix":"10.3390","volume":"11","author":[{"given":"Georgia","family":"Diakoudi","sequence":"first","affiliation":[{"name":"Dipartimento di Medicina Veterinaria, Universit\u00e0 Aldo Moro di Bari, S.p per Casamassima km 3, 70010 Valenzano, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gianvito","family":"Lanave","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Veterinaria, Universit\u00e0 Aldo Moro di Bari, S.p per Casamassima km 3, 70010 Valenzano, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8497-9708","authenticated-orcid":false,"given":"Ana","family":"Moreno","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Lombardia e dell\u2019Emilia Romagna, Via Bianchi 9, 25124 Brescia, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiara","family":"Chiapponi","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Lombardia e dell\u2019Emilia Romagna, Via Bianchi 9, 25124 Brescia, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enrica","family":"Sozzi","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Lombardia e dell\u2019Emilia Romagna, Via Bianchi 9, 25124 Brescia, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alice","family":"Prosperi","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Lombardia e dell\u2019Emilia Romagna, Via Bianchi 9, 25124 Brescia, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vittorio","family":"Larocca","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Veterinaria, Universit\u00e0 Aldo Moro di Bari, S.p per Casamassima km 3, 70010 Valenzano, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michele","family":"Losurdo","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Veterinaria, Universit\u00e0 Aldo Moro di Bari, S.p per Casamassima km 3, 70010 Valenzano, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6348-7969","authenticated-orcid":false,"given":"Nicola","family":"Decaro","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Veterinaria, Universit\u00e0 Aldo Moro di Bari, S.p per Casamassima km 3, 70010 Valenzano, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5740-6947","authenticated-orcid":false,"given":"Vito","family":"Martella","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Veterinaria, Universit\u00e0 Aldo Moro di Bari, S.p per Casamassima km 3, 70010 Valenzano, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7646-3592","authenticated-orcid":false,"given":"Antonio","family":"Lavazza","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Lombardia e dell\u2019Emilia Romagna, Via Bianchi 9, 25124 Brescia, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5775-6058","authenticated-orcid":false,"given":"Davide","family":"Lelli","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Lombardia e dell\u2019Emilia Romagna, Via Bianchi 9, 25124 Brescia, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,6,6]]},"reference":[{"key":"ref_1","unstructured":"King, A., Adams, M., Carstens, E., and Lefkowitz, E. (2011). Family Adenoviridae. Virus Taxonomy: Classification and Nomenclature of Viruses: Ninth Report of the International Committee on Taxonomy of Viruses, Elsevier."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1099\/jgv.0.000739","article-title":"Novel bat adenoviruses with low G+C content shed new light on the evolution of adenoviruses","volume":"98","author":"Tan","year":"2017","journal-title":"J. Gen. Virol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2895","DOI":"10.1099\/vir.0.19497-0","article-title":"Genetic content and evolution of adenoviruses","volume":"84","author":"Davison","year":"2003","journal-title":"J. Gen. Virol."},{"key":"ref_4","unstructured":"(2019, April 02). International Committee on Taxonomy of Viruses (ICTV) Virus Taxonomy: 2018b Release. Available online: http:\/\/talk.ictvonline.org\/taxonomy\/."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1186\/1743-422X-11-25","article-title":"High prevalence and diversity of species D adenoviruses (HAdV-D) in human populations of four Sub-Saharan countries","volume":"11","author":"Pauly","year":"2014","journal-title":"Virol. J."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1044","DOI":"10.3201\/eid2206.151898","article-title":"Human adenovirus associated with severe respiratory infection, Oregon, USA, 2013-2014","volume":"22","author":"Chommanard","year":"2016","journal-title":"Emerg. Infect. Dis."},{"key":"ref_7","first-page":"3","article-title":"Molecular Evolution of Adenoviruses","volume":"272","author":"Harrach","year":"2003","journal-title":"Immunology"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1812","DOI":"10.1038\/srep01812","article-title":"Molecular evolution of human adenoviruses","volume":"3","author":"Robinson","year":"2013","journal-title":"Sci. Rep."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2072","DOI":"10.1093\/molbev\/msv090","article-title":"Multiple cross-species transmission events of human adenoviruses (HAdV) during hominine evolution","volume":"32","author":"Hoppe","year":"2015","journal-title":"Mol. Biol. Evol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1128\/CMR.00017-06","article-title":"Bats: Important reservoir hosts of emerging viruses","volume":"19","author":"Calisher","year":"2006","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.coviro.2012.11.006","article-title":"Bats and their virome: An important source of emerging viruses capable of infecting humans","volume":"3","author":"Smith","year":"2013","journal-title":"Curr. Opin. Virol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1126\/science.1118391","article-title":"Bats are natural reservoirs of SARS-like coronaviruses","volume":"310","author":"Li","year":"2005","journal-title":"Science"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1990","DOI":"10.3201\/eid1612.091790","article-title":"Characterization of Nipah virus from naturally infected Pteropus vampyrus bats, Malaysia","volume":"16","author":"Rahman","year":"2010","journal-title":"Emerg. Infect. Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1111\/zph.12003","article-title":"Molecular Epidemiology of Bat Lyssaviruses in Europe","volume":"60","author":"Mcelhinney","year":"2013","journal-title":"Zoonoses Public Health"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2974","DOI":"10.3390\/v6082974","article-title":"Lyssaviruses and bats: Emergence and zoonotic threat","volume":"6","author":"Banyard","year":"2014","journal-title":"Viruses"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Fischer, K., dos Reis, V.P., and Balkema-Buschmann, A. (2017). Bat astroviruses: Towards understanding the transmission dynamics of a neglected virus family. Viruses, 9.","DOI":"10.3390\/v9020034"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1911","DOI":"10.3390\/v6051911","article-title":"Vampire bat rabies: Ecology, epidemiology and control","volume":"6","author":"Johnson","year":"2014","journal-title":"Viruses"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1590\/0074-02760150048","article-title":"Bats and zoonotic viruses: Can we confidently link bats with emerging deadly viruses?","volume":"110","author":"Moratelli","year":"2015","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_19","first-page":"35","article-title":"Bats and emerging infections: An ecological and virological puzzle","volume":"972","year":"2017","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"347","DOI":"10.3201\/eid1402.070932","article-title":"Isolation of novel adenovirus from fruit bat (Pteropus dasymallus yayeyamae)","volume":"14","author":"Maeda","year":"2008","journal-title":"Emerg. Infect. Dis."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1625","DOI":"10.1099\/jgv.0.000470","article-title":"Novel bat adenoviruses with an extremely large E3 gene","volume":"97","author":"Tan","year":"2016","journal-title":"J. Gen. Virol."},{"key":"ref_22","first-page":"e01504","article-title":"Evolution and Cryo-electron Microscopy Capsid Structure of a North American Bat Adenovirus and Its Relationship to Other Mastadenoviruses","volume":"91","author":"Hackenbrack","year":"2016","journal-title":"J. Virol."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Ogawa, H., Kajihara, M., Nao, N., Shigeno, A., Fujikura, D., Hang\u2019Ombe, B.M., Mweene, A.S., Mutemwa, A., Squarre, D., and Yamada, M. (2017). Characterization of a novel bat adenovirus isolated from straw-colored fruit bat (Eidolon helvum). Viruses, 9.","DOI":"10.3390\/v9120371"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2052","DOI":"10.3201\/eid1512.090646","article-title":"New adenovirus in bats, Germany","volume":"15","author":"Sonntag","year":"2009","journal-title":"Emerg. Infect. Dis."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"3889","DOI":"10.1128\/JVI.02497-09","article-title":"Host range, prevalence, and genetic diversity of adenoviruses in bats","volume":"84","author":"Li","year":"2010","journal-title":"J. Virol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1888","DOI":"10.1128\/JVI.05974-11","article-title":"Genome analysis of bat adenovirus 2: Indications of interspecies transmission","volume":"86","author":"Kohl","year":"2012","journal-title":"J. Virol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1016\/j.vetmic.2012.11.015","article-title":"Molecular epidemiology of canine adenovirus type 1 and type 2 in free-ranging red foxes (Vulpes vulpes) in Italy","volume":"162","author":"Balboni","year":"2013","journal-title":"Vet. Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1016\/j.meegid.2015.01.024","article-title":"Phylogenomic characterization of California sea lion adenovirus-1","volume":"31","author":"Gulland","year":"2015","journal-title":"Infect. Genet. Evol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"13366","DOI":"10.1128\/JVI.78.23.13366-13369.2004","article-title":"Detection and Analysis of Six Lizard Adenoviruses by Consensus Primer PCR Provides Further Evidence of a Reptilian Origin for the Atadenoviruses","volume":"78","author":"Wellehan","year":"2004","journal-title":"J. Virol."},{"key":"ref_30","first-page":"581","article-title":"Rod-shaped virus-like particles in intestinal contents of three avian species","volume":"126","author":"Lavazza","year":"1990","journal-title":"Vet. Rec."},{"key":"ref_31","unstructured":"Andrews, S. (2019, May 01). FastQC\u2014A Quality Control Tool for High Throughput Sequence Data. Available online: http:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc\/."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"5749","DOI":"10.1128\/JVI.02281-08","article-title":"A novel cardiotropic murine adenovirus representing a distinct species of mastadenoviruses","volume":"83","author":"Klempa","year":"2009","journal-title":"J. Virol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1099\/vir.0.19697-0","article-title":"Dna sequencing and analysis of the right-hand part of the genome of the unique bovine adenovirus type 10","volume":"85","author":"Ursu","year":"2004","journal-title":"J. Gen. Virol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1016\/0888-7543(91)90071-L","article-title":"Searching Protein Sequence Libraries: Comparison of the Sensitivity and Selectivity of the Smith-Waterman and FASTA Algorithms","volume":"3","author":"Pearson","year":"1991","journal-title":"Genomics"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1007\/s12250-016-3930-2","article-title":"Molecular detection of viruses in Kenyan bats and discovery of novel astroviruses, caliciviruses and rotaviruses","volume":"32","author":"Waruhiu","year":"2017","journal-title":"Virol. Sin."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Iglesias-Caballero, M., Juste, J., V\u00e1zquez-Mor\u00f3n, S., Falcon, A., Aznar-Lopez, C., Ib\u00e1\u00f1ez, C., Pozo, F., Ruiz, G., Berciano, J., and Garin, I. (2018). New Adenovirus Groups in Western Palaearctic Bats. Viruses, 10.","DOI":"10.3390\/v10080443"},{"key":"ref_38","unstructured":"Cortez-Hinojosa, G., Adkesson, M.J., C\u00e1rdenas-Alayza, S., Seguel, M., Pav\u00e9s, H., and Wellehan, J. (2016, January 21\u201326). Effect of environmental stress of an el ni\u00f1o event on adenoviral diversity in marine animal rookeries. Proceedings of the AAZV Annual Conference, Virginia Beach, VA, USA."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1556\/004.48.2000.4.11","article-title":"Reptile Adenoviruses in Cattle?","volume":"48","author":"Harrach","year":"2000","journal-title":"Acta Vet. Hung."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.vetmic.2018.05.025","article-title":"Sequential circulation of canine adenoviruses 1 and 2 in captive wild carnivores, France","volume":"221","author":"Dowgier","year":"2018","journal-title":"Vet. Microbiol."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/11\/6\/523\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T12:56:35Z","timestamp":1760187395000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/11\/6\/523"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,6,6]]},"references-count":40,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2019,6]]}},"alternative-id":["v11060523"],"URL":"https:\/\/doi.org\/10.3390\/v11060523","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,6,6]]}}}