{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T12:42:03Z","timestamp":1791895323701,"version":"4.3.4"},"reference-count":14,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2019,6,28]],"date-time":"2019-06-28T00:00:00Z","timestamp":1561680000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009037","name":"Science and Technology Research Partnership for Sustainable Development","doi-asserted-by":"publisher","award":["JP18jm0110019"],"award-info":[{"award-number":["JP18jm0110019"]}],"id":[{"id":"10.13039\/501100009037","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP18fm0108008"],"award-info":[{"award-number":["JP18fm0108008"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>The recent large outbreaks of Ebola virus disease (EVD) in West Africa and the Democratic Republic of the Congo (DRC) have highlighted the need for rapid diagnostic tests to control this disease. In this study, we clinically evaluated a previously developed immunochromatography-based kit, QuickNaviTM-Ebola. During the 2018 outbreaks in DRC, 928 blood samples from EVD-suspected cases were tested with QuickNaviTM-Ebola and the WHO-approved GeneXpert. The sensitivity and specificity of QuickNaviTM-Ebola, estimated by comparing it to GeneXpert-confirmed cases, were 85% (68\/80) and 99.8% (846\/848), respectively. These results indicate the practical reliability of QuickNaviTM-Ebola for point-of-care diagnosis of EVD.<\/jats:p>","DOI":"10.3390\/v11070589","type":"journal-article","created":{"date-parts":[[2019,6,28]],"date-time":"2019-06-28T11:20:26Z","timestamp":1561720826000},"page":"589","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Clinical Evaluation of QuickNaviTM-Ebola in the 2018 Outbreak of Ebola Virus Disease in the Democratic Republic of the Congo"],"prefix":"10.3390","volume":"11","author":[{"given":"Sheila","family":"Makiala","sequence":"first","affiliation":[{"name":"Institut National de Recherche Biom\u00e9dicale, Avenue de la D\u00e9mocratie, Kinshasa\/Gombe-P.O. Box 1197, Kinshasa I, Democratic Republic of the Congo"},{"name":"Service de Microbiologie, Cliniques Universitaires de Kinshasa, Kinshasa, Democratic Republic of the Congo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6836-8704","authenticated-orcid":false,"given":"Daniel","family":"Mukadi","sequence":"additional","affiliation":[{"name":"Institut National de Recherche Biom\u00e9dicale, Avenue de la D\u00e9mocratie, Kinshasa\/Gombe-P.O. Box 1197, Kinshasa I, Democratic Republic of the Congo"},{"name":"Service de Microbiologie, Cliniques Universitaires de Kinshasa, Kinshasa, Democratic Republic of the Congo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9377-4766","authenticated-orcid":false,"given":"Anja","family":"De Weggheleire","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Institute of Tropical Medicine, Antwerp, Belgium"},{"name":"Outbreak Research team, Institute of Tropical Medicine, Antwerp, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shino","family":"Muramatsu","sequence":"additional","affiliation":[{"name":"Denka Seiken Co., Ltd., 1359-1, Kagamida, Kigoshi, Gosen, Niigata 959-1695, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisuke","family":"Kato","sequence":"additional","affiliation":[{"name":"Denka Seiken Co., Ltd., 1359-1, Kagamida, Kigoshi, Gosen, Niigata 959-1695, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koichi","family":"Inano","sequence":"additional","affiliation":[{"name":"Denka Seiken Co., Ltd., 1359-1, Kagamida, Kigoshi, Gosen, Niigata 959-1695, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumio","family":"Gondaira","sequence":"additional","affiliation":[{"name":"Denka Seiken Co., Ltd., 1359-1, Kagamida, Kigoshi, Gosen, Niigata 959-1695, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahiro","family":"Kajihara","sequence":"additional","affiliation":[{"name":"Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, Kita-20, Nishi-10, Kita-ku, Sapporo 001-0020, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reiko","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, Kita-20, Nishi-10, Kita-ku, Sapporo 001-0020, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2182-4319","authenticated-orcid":false,"given":"Katendi","family":"Changula","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, the University of Zambia, Great East Road Campus, Lusaka, Zambia"},{"name":"Africa Center of Excellence for Infectious Diseases of Humans and Animals, the University of Zambia, Lusaka, Zambia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron","family":"Mweene","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, the University of Zambia, Great East Road Campus, Lusaka, Zambia"},{"name":"Africa Center of Excellence for Infectious Diseases of Humans and Animals, the University of Zambia, Lusaka, Zambia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Placide","family":"Mbala-Kingebeni","sequence":"additional","affiliation":[{"name":"Institut National de Recherche Biom\u00e9dicale, Avenue de la D\u00e9mocratie, Kinshasa\/Gombe-P.O. Box 1197, Kinshasa I, Democratic Republic of the Congo"},{"name":"Service de Microbiologie, Cliniques Universitaires de Kinshasa, Kinshasa, Democratic Republic of the Congo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Jacques","family":"Muyembe-Tamfum","sequence":"additional","affiliation":[{"name":"Institut National de Recherche Biom\u00e9dicale, Avenue de la D\u00e9mocratie, Kinshasa\/Gombe-P.O. Box 1197, Kinshasa I, Democratic Republic of the Congo"},{"name":"Service de Microbiologie, Cliniques Universitaires de Kinshasa, Kinshasa, Democratic Republic of the Congo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justin","family":"Masumu","sequence":"additional","affiliation":[{"name":"Institut National de Recherche Biom\u00e9dicale, Avenue de la D\u00e9mocratie, Kinshasa\/Gombe-P.O. Box 1197, Kinshasa I, Democratic Republic of the Congo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steve","family":"Ahuka","sequence":"additional","affiliation":[{"name":"Institut National de Recherche Biom\u00e9dicale, Avenue de la D\u00e9mocratie, Kinshasa\/Gombe-P.O. Box 1197, Kinshasa I, Democratic Republic of the Congo"},{"name":"Service de Microbiologie, Cliniques Universitaires de Kinshasa, Kinshasa, Democratic Republic of the Congo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2464-6642","authenticated-orcid":false,"given":"Ayato","family":"Takada","sequence":"additional","affiliation":[{"name":"Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, Kita-20, Nishi-10, Kita-ku, Sapporo 001-0020, Japan"},{"name":"School of Veterinary Medicine, the University of Zambia, Great East Road Campus, Lusaka, Zambia"},{"name":"Africa Center of Excellence for Infectious Diseases of Humans and Animals, the University of Zambia, Lusaka, Zambia"},{"name":"Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education, Hokkaido University, Kita-20, Nishi-10, Kita-ku, Sapporo 001-0020, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,6,28]]},"reference":[{"key":"ref_1","first-page":"923","article-title":"Filoviridae: Marburg and Ebola Viruses","volume":"Volume 1","author":"Knipe","year":"2013","journal-title":"Fields Virology"},{"key":"ref_2","unstructured":"(2019, June 17). Disease Outbreak News (DONs). Available online: https:\/\/www.who.int\/ebola\/situation-reports\/drc-2018\/en\/."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"S185","DOI":"10.1093\/infdis\/jiw252","article-title":"Development of an immunochromatography assay (QuickNavi-Ebola) to detect multiple species of ebolaviruses","volume":"214","author":"Yoshida","year":"2016","journal-title":"J. Infect. Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1038\/d41586-018-05389-2","article-title":"Speedy Ebola tests help contain Africa\u2019s latest outbreak","volume":"558","author":"Butler","year":"2018","journal-title":"Nature"},{"key":"ref_5","unstructured":"(2019, May 15). Ebola Research and Development Landscape of Clinical Candidates and Trials. Available online: http:\/\/www.who.int\/medicines\/ebola-treatment\/EbolaR_D_public-report_updt2015.pdf?ua=1."},{"key":"ref_6","unstructured":"(2018, November 09). FDA Authorizes Emergency Use of First Ebola Fingerstick Test with Portable Reader, Available online: https:\/\/www.fda.gov\/newsevents\/newsroom\/pressannouncements\/ucm625502.htm."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1038\/d41586-019-00212-y","article-title":"Where are the Ebola diagnostics from last time?","volume":"565","author":"Cnops","year":"2019","journal-title":"Nature"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1016\/S1473-3099(19)30118-5","article-title":"Medical countermeasures during the 2018 Ebola virus disease outbreak in the North Kivu and Ituri Provinces of the Democratic Republic of the Congo: A rapid genomic assessment","volume":"19","author":"Aziza","year":"2019","journal-title":"Lancet Infect. Dis."},{"key":"ref_9","unstructured":"(2019, May 15). WHO Emergency Use Assessment and Listing for Ebola Virus Disease IVDs PUBLIC REPORT. Available online: https:\/\/www.who.int\/diagnostics_laboratory\/160324_final_public_report_ea_0023_021_00.pdf."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"4275","DOI":"10.1073\/pnas.1120453109","article-title":"Structural dissection of Ebola virus and its assembly determinants using cryo-electron tomography","volume":"109","author":"Bharat","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1038\/s41586-018-0630-0","article-title":"Cryo-EM structure of the Ebola virus nucleoprotein-RNA complex at 3.6 \u00c5 resolution","volume":"563","author":"Sugita","year":"2018","journal-title":"Nature"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.virusres.2013.05.004","article-title":"Mapping of conserved and species-specific antibody epitopes on the Ebola virus nucleoprotein","volume":"176","author":"Changula","year":"2013","journal-title":"Virus Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1007\/s00216-010-3661-4","article-title":"Development and application of lateral flow test strip technology for detection of infectious agents and chemical contaminants: A review","volume":"397","author":"Ngom","year":"2010","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"S761","DOI":"10.1093\/infdis\/jir294","article-title":"Filovirus outbreak detection and surveillance: Lessons from Bundibugyo","volume":"204","author":"MacNeil","year":"2011","journal-title":"J. Infect. Dis."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/11\/7\/589\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:02:01Z","timestamp":1760187721000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/11\/7\/589"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,6,28]]},"references-count":14,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2019,7]]}},"alternative-id":["v11070589"],"URL":"https:\/\/doi.org\/10.3390\/v11070589","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,6,28]]}}}