{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T17:58:02Z","timestamp":1791655082180,"version":"4.3.4"},"reference-count":30,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2019,7,13]],"date-time":"2019-07-13T00:00:00Z","timestamp":1562976000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["RO1 AI132633"],"award-info":[{"award-number":["RO1 AI132633"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Andes virus (ANDV) and Sin Nombre virus (SNV) are the main causative agents responsible for hantavirus cardiopulmonary syndrome (HCPS) in the Americas. HCPS is a severe respiratory disease with a high fatality rate for which there are no approved therapeutics or vaccines available. Some vaccine approaches for HCPS have been tested in preclinical models, but none have been tested in infectious models in regard to their ability to protect against multiple species of HCPS-causing viruses. Here, we utilize recombinant vesicular stomatitis virus-based (VSV) vaccines for Andes virus (ANDV) and Sin Nombre virus (SNV) and assess their ability to provide cross-protection in infectious challenge models. We show that, while both rVSV\u0394G\/ANDVGPC and rVSV\u0394G\/SNVGPC display attenuated growth as compared to wild type VSV, each vaccine is able to induce a cross-reactive antibody response. Both vaccines protected against both homologous and heterologous challenge with ANDV and SNV and prevented HCPS in a lethal ANDV challenge model. This study provides evidence that the development of a single vaccine against HCPS-causing hantaviruses could provide protection against multiple agents.<\/jats:p>","DOI":"10.3390\/v11070645","type":"journal-article","created":{"date-parts":[[2019,7,15]],"date-time":"2019-07-15T04:55:27Z","timestamp":1563166527000},"page":"645","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Vesicular Stomatitis Virus-Based Vaccines Provide Cross-Protection against Andes and Sin Nombre Viruses"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2635-5072","authenticated-orcid":false,"given":"Bryce M","family":"Warner","sequence":"first","affiliation":[{"name":"Zoonotic Diseases and Special Pathogens, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB R3E3R2, Canada"},{"name":"Department of Medical Microbiology, University of Manitoba, Winnipeg, MB R3E0J9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derek R","family":"Stein","sequence":"additional","affiliation":[{"name":"Zoonotic Diseases and Special Pathogens, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB R3E3R2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3119-0869","authenticated-orcid":false,"given":"Rohit K","family":"Jangra","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan M","family":"Slough","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrycja","family":"Sroga","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, University of Manitoba, Winnipeg, MB R3E0J9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Sloan","sequence":"additional","affiliation":[{"name":"Zoonotic Diseases and Special Pathogens, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB R3E3R2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathy L","family":"Frost","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, University of Manitoba, Winnipeg, MB R3E0J9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie","family":"Booth","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, University of Manitoba, Winnipeg, MB R3E0J9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kartik","family":"Chandran","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Safronetz","sequence":"additional","affiliation":[{"name":"Zoonotic Diseases and Special Pathogens, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB R3E3R2, Canada"},{"name":"Department of Medical Microbiology, University of Manitoba, Winnipeg, MB R3E0J9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,7,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1128\/CMR.00062-09","article-title":"A Global Perspective on Hantavirus Ecology, Epidemiology, and Disease","volume":"23","author":"Jonsson","year":"2010","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"124","DOI":"10.14745\/ccdr.v41i06a02","article-title":"Hantavirus Pulmonary Syndrome in Canada: An Overview of Clinical Features, Diagnostics, Epidemiology and Prevention","volume":"41","author":"Drebot","year":"2015","journal-title":"Can. Commun. Dis. Rep."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2446","DOI":"10.1128\/JVI.01057-08","article-title":"Ecology, Genetic Diversity, and Phylogeographic Structure of Andes Virus in Humans and Rodents in Chile","volume":"83","author":"Medina","year":"2009","journal-title":"J. Virol."},{"key":"ref_4","first-page":"1629","article-title":"Person-to-Person Household and Nosocomial Transmission of Andes Hantavirus, Southern Chile, 2011","volume":"20","author":"Calvo","year":"2014","journal-title":"Emerg. Infect. Dis."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Safronetz, D., Haddock, E., Feldmann, F., Ebihara, H., and Feldmann, H. (2011). In Vitro and in Vivo Activity of Ribavirin Against Andes Virus Infection. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0023560"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1307","DOI":"10.1086\/425007","article-title":"Placebo-Controlled, Double-Blind Trial of Intravenous Ribavirin for the Treatment of Hantavirus Cardiopulmonary Syndrome in North America","volume":"39","author":"Mertz","year":"2004","journal-title":"Clin. Infect. Dis."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"377","DOI":"10.3851\/IMP2875","article-title":"A Non-Randomized Multicentre Trial of Human Immune Plasma for Treatment of Hantavirus Cardiopulmonary Syndrome Caused by Andes Virus","volume":"20","author":"Vial","year":"2015","journal-title":"Antivir Ther."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1016\/j.vaccine.2016.01.031","article-title":"Long-Term Immunogenicity and Safety of Inactivated Hantaan Virus Vaccine (Hantavax) in Healthy Adults","volume":"34","author":"Song","year":"2016","journal-title":"Vaccine"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1745","DOI":"10.1099\/0022-1317-83-7-1745","article-title":"Genetic Vaccines Protect Against Sin Nombre Hantavirus Challenge in the Deer Mouse (Peromyscus Maniculatus)","volume":"83","author":"Bharadwaj","year":"2002","journal-title":"J. Gen. Virol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"4314","DOI":"10.1016\/j.vaccine.2013.07.025","article-title":"A Novel Sin Nombre Virus DNA Vaccine and its Inclusion in a Candidate Pan-Hantavirus Vaccine Against Hantavirus Pulmonary Syndrome (HPS) and Hemorrhagic Fever with Renal Syndrome (HFRS)","volume":"31","author":"Hooper","year":"2013","journal-title":"Vaccine"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"12781","DOI":"10.1128\/JVI.00794-11","article-title":"Vesicular Stomatitis Virus-Based Vaccine Protects Hamsters Against Lethal Challenge with Andes Virus","volume":"85","author":"Brown","year":"2011","journal-title":"J. Virol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"7285","DOI":"10.1128\/JVI.00373-09","article-title":"Adenovirus Vectors Expressing Hantavirus Proteins Protect Hamsters Against Lethal Challenge with Andes Virus","volume":"83","author":"Safronetz","year":"2009","journal-title":"J. Virol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1016\/j.antiviral.2012.06.002","article-title":"The Syrian Hamster Model of Hantavirus Pulmonary Syndrome","volume":"95","author":"Safronetz","year":"2012","journal-title":"Antivir. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"8469","DOI":"10.1128\/JVI.75.18.8469-8477.2001","article-title":"DNA Vaccination with the Hantaan Virus M Gene Protects Hamsters Against Three of Four HFRS Hantaviruses and Elicits a High-Titer Neutralizing Antibody Response in Rhesus Monkeys","volume":"75","author":"Hooper","year":"2001","journal-title":"J. Virol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"6417","DOI":"10.1128\/jvi.69.10.6417-6423.1995","article-title":"A Vaccinia Virus-Vectored Hantaan Virus Vaccine Protects Hamsters from Challenge with Hantaan and Seoul Viruses but Not Puumala Virus","volume":"69","author":"Chu","year":"1995","journal-title":"J. Virol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"9894","DOI":"10.1128\/JVI.77.18.9894-9905.2003","article-title":"Active and Passive Vaccination Against Hantavirus Pulmonary Syndrome with Andes Virus M Genome Segment-Based DNA Vaccine","volume":"77","author":"Custer","year":"2003","journal-title":"J. Virol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"200","DOI":"10.2174\/1566523214666140522122633","article-title":"A Hantavirus Pulmonary Syndrome (HPS) DNA Vaccine Delivered using a Spring-Powered Jet Injector Elicits a Potent Neutralizing Antibody Response in Rabbits and Nonhuman Primates","volume":"14","author":"Kwilas","year":"2014","journal-title":"Curr. Gene Ther."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/j.virol.2005.11.035","article-title":"Hantaan\/Andes Virus DNA Vaccine Elicits a Broadly Cross-Reactive Neutralizing Antibody Response in Nonhuman Primates","volume":"347","author":"Hooper","year":"2006","journal-title":"Virology"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"4778","DOI":"10.1128\/JVI.03291-12","article-title":"Hamster-Adapted Sin Nombre Virus Causes Disseminated Infection and Efficiently Replicates in Pulmonary Endothelial Cells without Signs of Disease","volume":"87","author":"Safronetz","year":"2013","journal-title":"J. Virol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"e00801","DOI":"10.1128\/mBio.00801-15","article-title":"Haploid Genetic Screen Reveals a Profound and Direct Dependence on Cholesterol for Hantavirus Membrane Fusion","volume":"6","author":"Kleinfelter","year":"2015","journal-title":"MBio"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Safronetz, D., Mire, C., Rosenke, K., Feldmann, F., Haddock, E., Geisbert, T., and Feldmann, H. (2015). A Recombinant Vesicular Stomatitis Virus-Based Lassa Fever Vaccine Protects Guinea Pigs and Macaques Against Challenge with Geographically and Genetically Distinct Lassa Viruses. PLoS Negl. Trop. Dis., 9.","DOI":"10.1371\/journal.pntd.0003736"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1038\/s41586-018-0702-1","article-title":"Protocadherin-1 is Essential for Cell Entry by New World Hantaviruses","volume":"563","author":"Jangra","year":"2018","journal-title":"Nature"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.antiviral.2017.09.005","article-title":"Human Polyclonal Antibodies Produced in Transchromosomal Cattle Prevent Lethal Zika Virus Infection and Testicular Atrophy in Mice","volume":"146","author":"Stein","year":"2017","journal-title":"Antivir. Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1038\/s41541-019-0104-x","article-title":"A Recombinant Vesicular Stomatitis-Based Lassa Fever Vaccine Elicits Rapid and Long-Term Protection from Lethal Lassa Virus Infection in Guinea Pigs","volume":"4","author":"Stein","year":"2019","journal-title":"NPJ Vaccines"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1586\/erv.12.15","article-title":"Vaccines for Hantaviruses: Progress and Issues","volume":"11","author":"Schmaljohn","year":"2012","journal-title":"Expert Rev. Vaccines"},{"key":"ref_26","first-page":"373","article-title":"Vaccines Against Hantaviruses. Expert Rev","volume":"1","author":"Hjelle","year":"2002","journal-title":"Vaccines"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1007\/5584_2016_135","article-title":"Syrian Hamsters as a Small Animal Model for Emerging Infectious Diseases: Advances in Immunologic Methods","volume":"972","author":"Warner","year":"2017","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_28","unstructured":"Mathias, S., Jens, J., Detlev, K.H., Andreas, R., and Rainer, U.G. (2014). Hantavirus emergence in rodents, insectivores, and bats: What comes next?. The Role of Animals in Emerging Viral Diseases, Academic Press."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"5458","DOI":"10.1128\/JVI.78.10.5458-5465.2004","article-title":"Properties of Replication-Competent Vesicular Stomatitis Virus Vectors Expressing Glycoproteins of Filoviruses and Arenaviruses","volume":"78","author":"Garbutt","year":"2004","journal-title":"J. Virol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1128\/JVI.02906-13","article-title":"A lethal disease model for hantavirus pulmonary syndrome in immunosuppressed Syrian hamsters infected with Sin Nombre virus","volume":"88","author":"Brocato","year":"2014","journal-title":"J. Virol."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/11\/7\/645\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:05:18Z","timestamp":1760187918000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/11\/7\/645"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7,13]]},"references-count":30,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2019,7]]}},"alternative-id":["v11070645"],"URL":"https:\/\/doi.org\/10.3390\/v11070645","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,7,13]]}}}