{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T00:16:34Z","timestamp":1783037794812,"version":"3.54.6"},"reference-count":33,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2019,7,29]],"date-time":"2019-07-29T00:00:00Z","timestamp":1564358400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Aseptic meningitis is a common viral infection associated with human enteroviruses. The aim of the present study was to identify and characterize the enteroviruses associated with outbreaks and sporadic cases of aseptic meningitis that occurred in different regions of Brazil between 2013 and 2017. Cerebrospinal fluids obtained from patients admitted to public health facilities were analyzed. A total of 303 patients were positive for Human Enteroviruses (EV) by cell culture isolation with a median isolation rate throughout the year of 12%. We were able to identify enterovirus serotypes in 295 clinical specimens. Nineteen different serotypes were identified; the large majority corresponded to HEV-B species. Echovirus 30 (E-30) and Echovirus 6 (E-6) were the most prevalent genotypes (66.8%). Sequence analysis suggested that circulating E-30 was closely related to E-30 from other American countries; while E-6 was derived from Europe. Most of the patients consisted of children \u2264 15 years old. The temporal distribution of all aseptic meningitis and EV-positive cases showed an obvious seasonal pattern during autumn. Our results have provided valuable information about the enteroviral etiology of the aseptic meningitis cases in Brazil pointing to the importance of enterovirus surveillance in neurological diseases.<\/jats:p>","DOI":"10.3390\/v11080690","type":"journal-article","created":{"date-parts":[[2019,7,29]],"date-time":"2019-07-29T11:20:18Z","timestamp":1564399218000},"page":"690","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Identification and Phylogenetic Characterization of Human Enteroviruses Isolated from Cases of Aseptic Meningitis in Brazil, 2013\u20132017"],"prefix":"10.3390","volume":"11","author":[{"given":"Emanuelle","family":"Ramalho","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Enterov\u00edrus, Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro 21040900, Brazil"},{"name":"Departamento de Neurologia, Faculdade de Medicina, Hospital Universit\u00e1rio Ant\u00f4nio Pedro, Universidade Federal Fluminense, Rio de Janeiro 24033900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Ivanildo","family":"Sousa","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Enterov\u00edrus, Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro 21040900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda","family":"Burlandy","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Enterov\u00edrus, Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro 21040900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eliane","family":"Costa","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Enterov\u00edrus, Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro 21040900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda","family":"Dias","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Enterov\u00edrus, Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro 21040900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roseane","family":"Serrano","sequence":"additional","affiliation":[{"name":"Secretaria Estadual de Sa\u00fade de Pernambuco, Recife, PE 50751530, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Setor de Virologia, Laborat\u00f3rio Central de Sa\u00fade P\u00fablica de Pernambuco, Recife, PE 50751530, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renato","family":"Lopes","sequence":"additional","affiliation":[{"name":"Secretaria Estadual de Sa\u00fade do Paran\u00e1, Brazil\u2014Divis\u00e3o de Vigil\u00e2ncia de doen\u00e7as transmiss\u00edveis, Paran\u00e1 80230140, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Debur","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Central de Sa\u00fade P\u00fablica do Estado do Paran\u00e1, Curitiba, Paran\u00e1 80060190, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marion","family":"Burger","sequence":"additional","affiliation":[{"name":"Centro de Epidemiologia da SMS, Curitiba, Paran\u00e1 80060130, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina","family":"Riediger","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Central de Sa\u00fade P\u00fablica do Estado do Paran\u00e1, Curitiba, Paran\u00e1 80060190, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria L.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Laboratory of Technological Development in Virology, Oswaldo Cruz, Institute, Fiocruz, Rio de Janeiro 21040900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Osvaldo","family":"Nascimento","sequence":"additional","affiliation":[{"name":"Departamento de Neurologia, Faculdade de Medicina, Hospital Universit\u00e1rio Ant\u00f4nio Pedro, Universidade Federal Fluminense, Rio de Janeiro 24033900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edson E.","family":"da Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Enterov\u00edrus, Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro 21040900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,7,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1016\/j.meegid.2012.10.016","article-title":"Picornavirus and enterovirus diversity with associated human diseases","volume":"14","author":"Tapparel","year":"2013","journal-title":"Infect. 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