{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T03:42:55Z","timestamp":1760240575200,"version":"build-2065373602"},"reference-count":21,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2019,8,6]],"date-time":"2019-08-06T00:00:00Z","timestamp":1565049600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003762","name":"Institut Pasteur","doi-asserted-by":"publisher","award":["PTR 505"],"award-info":[{"award-number":["PTR 505"]}],"id":[{"id":"10.13039\/501100003762","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Genetic variants of Thailand orthohantavirus (THAIV) have been recently reported from rodents in South-East Asia and in islands from the South-West part of the Indian Ocean. In order to detect THAIV and its variants, we developed a sensitive and specific real-time RT-PCR targeting the S segment. Our assay was developed in two different RT-PCR systems that gave similar results in terms of sensitivity. Moreover, our results demonstrated a specificity of 100%.<\/jats:p>","DOI":"10.3390\/v11080718","type":"journal-article","created":{"date-parts":[[2019,8,7]],"date-time":"2019-08-07T03:09:08Z","timestamp":1565147348000},"page":"718","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Fast, Sensitive and Specific Detection of Thailand orthohantavirus and Its Variants Using One-Step Real-Time Reverse-Transcription Polymerase Chain Reaction Assay"],"prefix":"10.3390","volume":"11","author":[{"given":"Vololoniaina","family":"Raharinosy","sequence":"first","affiliation":[{"name":"Virology Unit, Institut Pasteur de Madagascar, 101 Antananarivo, Madagascar"},{"name":"Ecole Doctorale Science de la Vie et de l\u2019Environnement, Facult\u00e9 des Sciences, Universit\u00e9 d\u2019Antananarivo, 101 Antananarivo, Madagascar"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1107-0859","authenticated-orcid":false,"given":"Jean-Michel","family":"Heraud","sequence":"additional","affiliation":[{"name":"Virology Unit, Institut Pasteur de Madagascar, 101 Antananarivo, Madagascar"},{"name":"Ecole Doctorale Science de la Vie et de l\u2019Environnement, Facult\u00e9 des Sciences, Universit\u00e9 d\u2019Antananarivo, 101 Antananarivo, Madagascar"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harinirina Aina","family":"Rabemananjara","sequence":"additional","affiliation":[{"name":"Virology Unit, Institut Pasteur de Madagascar, 101 Antananarivo, Madagascar"},{"name":"Ecole Doctorale Science de la Vie et de l\u2019Environnement, Facult\u00e9 des Sciences, Universit\u00e9 d\u2019Antananarivo, 101 Antananarivo, Madagascar"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"Telfer","sequence":"additional","affiliation":[{"name":"Plague Unit, Institut Pasteur de Madagascar, 101 Antananarivo, Madagascar"},{"name":"Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen AB24 3FX, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danielle Aurore Doll","family":"Rakoto","sequence":"additional","affiliation":[{"name":"Ecole Doctorale Science de la Vie et de l\u2019Environnement, Facult\u00e9 des Sciences, Universit\u00e9 d\u2019Antananarivo, 101 Antananarivo, Madagascar"},{"name":"D\u00e9partement de Biochimie Fondamentale et Appliqu\u00e9e, Facult\u00e9 des Sciences, Universit\u00e9 d\u2019Antananarivo, 101 Antananarivo, Madagascar"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Filippone","sequence":"additional","affiliation":[{"name":"Virology Unit, Institut Pasteur de Madagascar, 101 Antananarivo, Madagascar"},{"name":"Ecole Doctorale Science de la Vie et de l\u2019Environnement, Facult\u00e9 des Sciences, Universit\u00e9 d\u2019Antananarivo, 101 Antananarivo, Madagascar"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Marc","family":"Reynes","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Biologie des Infections Virales Emergentes, Institut Pasteur, 69000 Lyon, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,8,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Abudurexiti, A., Adkins, S., Alioto, D., Alkhovsky, S.V., Av\u0161i\u010d-\u017dupanc, T., Ballinger, M.J., Bente, D.A., Beer, M., Bergeron, \u00c9., and Blair, C.D. 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