{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T08:34:29Z","timestamp":1791362069194,"version":"4.3.1"},"reference-count":52,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2019,8,6]],"date-time":"2019-08-06T00:00:00Z","timestamp":1565049600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100013206","name":"Food Institutional Research Measure","doi-asserted-by":"publisher","award":["14\/F\/881"],"award-info":[{"award-number":["14\/F\/881"]}],"id":[{"id":"10.13039\/100013206","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Listeria monocytogenes is a ubiquitous Gram-positive bacterium that is a major concern for food business operators because of its pathogenicity and ability to form biofilms in food production environments. Bacteriophages (phages) have been evaluated as biocontrol agents for L. monocytogenes in a number of studies and, indeed, certain phages have been approved for use as anti-listerial agents in food processing environments (ListShield and PhageGuard Listex). Endolysins are proteins produced by phages in the host cell. They cleave the peptidoglycan cell wall, thus allowing release of progeny phage into the environment. In this study, the amidase domain of the phage vB_LmoS_293 endolysin (293-amidase) was cloned and expressed in Escherichia. coli (E. coli). Muralytic activity at different concentrations, pH and temperature values, lytic spectrum and activity against biofilms was determined for the purified 293-amidase protein. The results showed activity on autoclaved cells at three different temperatures (20 \u00b0C, 37 \u00b0C and 50 \u00b0C), with a wider specificity (L. monocytogenes 473 and 3099, a serotype 4b and serogroup 1\/2b-3b-7, respectively) compared to the phage itself, which targets only L. monocytogenes serotypes 4b and 4e. The protein also inhibits biofilm formation on abiotic surfaces. These results show the potential of using recombinant antimicrobial proteins against pathogens in the food production environment.<\/jats:p>","DOI":"10.3390\/v11080722","type":"journal-article","created":{"date-parts":[[2019,8,7]],"date-time":"2019-08-07T10:56:38Z","timestamp":1565175398000},"page":"722","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Inhibition of L. monocytogenes Biofilm Formation by the Amidase Domain of the Phage vB_LmoS_293 Endolysin"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8752-3231","authenticated-orcid":false,"given":"Vincenzo","family":"Pennone","sequence":"first","affiliation":[{"name":"Teagasc Food Research Center, Moorepark, Fermoy, Co. Cork P61 C996, Ireland"},{"name":"Cork Institute of Technology, Bishopstown, Cork T12 P928, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Sanz-Gaitero","sequence":"additional","affiliation":[{"name":"Centro Nacional de Biotecnolog\u00eda (CNB-CSIC), 28049 Madrid, Spain"},{"name":"Cork Institute of Technology, Bishopstown, Cork T12 P928, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula","family":"O\u2019Connor","sequence":"additional","affiliation":[{"name":"Teagasc Food Research Center, Moorepark, Fermoy, Co. Cork P61 C996, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4116-9913","authenticated-orcid":false,"given":"Aidan","family":"Coffey","sequence":"additional","affiliation":[{"name":"Cork Institute of Technology, Bishopstown, Cork T12 P928, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3017-9622","authenticated-orcid":false,"given":"Kieran","family":"Jordan","sequence":"additional","affiliation":[{"name":"Teagasc Food Research Center, Moorepark, Fermoy, Co. Cork P61 C996, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark J.","family":"van Raaij","sequence":"additional","affiliation":[{"name":"Centro Nacional de Biotecnolog\u00eda (CNB-CSIC), 28049 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivia","family":"McAuliffe","sequence":"additional","affiliation":[{"name":"Teagasc Food Research Center, Moorepark, Fermoy, Co. Cork P61 C996, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,8,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1111\/jam.13337","article-title":"Inhibition of Listeria monocytogenes biofilms by bacteriocin-producing bacteria isolated from mushroom substrate","volume":"122","author":"Bolocan","year":"2017","journal-title":"J. Appl. 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