{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T00:41:34Z","timestamp":1790469694192,"version":"4.1.0"},"reference-count":44,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2019,8,22]],"date-time":"2019-08-22T00:00:00Z","timestamp":1566432000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["457664\/2013-4"],"award-info":[{"award-number":["457664\/2013-4"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Because of its ecological characteristics, the Caxiuan\u00e3 National Forest (FLONA) is a potential area of arbovirus circulation. The present study aimed to investigate the occurrence of arbovirus transmission cycles at FLONA de Caxiuan\u00e3. Five field trips were performed to capture mosquitoes and sylvatic vertebrates. For these vertebrates, we attempted viral isolation by cell monolayer inoculation from blood, and hemagglutination inhibition and further seroneutralization assays from sera. For mosquitoes, we performed tests of viral genome detection. A total of 338 vertebrates were captured, and the greatest representative was birds (251\/74.26%). A total of 16,725 mosquitoes were captured, distributed among 56 species. There were no viruses isolated by newborn mouse inoculation. Among birds, antibodies against Ilheus virus were the most prevalent. Catu virus, Caraparu virus, and Mucambo virus were the most prevalent among mammals and reptiles. Fragments of Mucambo virus, Ilheus virus, Bussuquara virus, and Rocio virus genome were detected in a pool of mosquito samples. These results of the study suggest the occurrence of arbovirus transmission cycles in the FLONA of Caxiuan\u00e3. The proximity of human populations with elements, involved in transmission cycles, makes surveillance necessary in this population to avoid dispersion of arboviruses to na\u00efve locations.<\/jats:p>","DOI":"10.3390\/v11090774","type":"journal-article","created":{"date-parts":[[2019,8,23]],"date-time":"2019-08-23T10:15:07Z","timestamp":1566555307000},"page":"774","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Investigation about the Occurrence of Transmission Cycles of Arbovirus in the Tropical Forest, Amazon Region"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7199-9304","authenticated-orcid":false,"given":"Pedro A.","family":"Ara\u00fajo","sequence":"first","affiliation":[{"name":"Institute of Biological Sciences, Federal University of Par\u00e1, Bel\u00e9m, PA 66075-110, Brazil"},{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, PA 67030-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria O.","family":"Freitas","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, PA 67030-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jannifer Oliveira","family":"Chiang","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, PA 67030-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franko Arruda","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, PA 67030-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liliane Leal","family":"Chagas","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, PA 67030-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samir Mansour","family":"Casseb","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, PA 67030-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandro Patroca","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, PA 67030-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5981-0065","authenticated-orcid":false,"given":"Joaquim Pinto","family":"Nunes-Neto","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, PA 67030-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Wilson","family":"Rosa-J\u00fanior","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, PA 67030-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruna Sena","family":"Nascimento","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, PA 67030-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3676-2288","authenticated-orcid":false,"given":"Leonardo Almeida","family":"Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, PA 67030-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thito Bezerra","family":"Paz","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, PA 67030-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Landeson L.","family":"Barros","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, PA 67030-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eliana P.","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, PA 67030-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raimunda S.","family":"Azevedo","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, PA 67030-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00edvia C.","family":"Martins","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, PA 67030-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milene S.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, PA 67030-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro F.","family":"Vasconcelos","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, PA 67030-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,8,22]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (1967). 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