{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T15:54:14Z","timestamp":1778860454359,"version":"3.51.4"},"reference-count":46,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2019,11,19]],"date-time":"2019-11-19T00:00:00Z","timestamp":1574121600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["PROEX"],"award-info":[{"award-number":["PROEX"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["CBB- APQ-04295-17 (EDITAL 007\/2017 - PROGRAMA DE PESQUISA PARA O SUS \/ PPSUS)"],"award-info":[{"award-number":["CBB- APQ-04295-17 (EDITAL 007\/2017 - PROGRAMA DE PESQUISA PARA O SUS \/ PPSUS)"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004809","name":"Financiadora de Estudos e Projetos","doi-asserted-by":"publisher","award":["FINEP-ZIKA institutional grant"],"award-info":[{"award-number":["FINEP-ZIKA institutional grant"]}],"id":[{"id":"10.13039\/501100004809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["PQ fellowship grant"],"award-info":[{"award-number":["PQ fellowship grant"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Dengue is currently one of the most important arbovirus infections worldwide. Early diagnosis is important for disease outcome, particularly for those afflicted with the severe forms of infection. The goal of this work was to identify conserved and polymorphic linear B-cell Dengue virus (DENV) epitopes that could be used for diagnostic purposes. To this end, we aligned the predicted viral proteome of the four DENV serotype and performed in silico B-cell epitope mapping. We developed a script in Perl integrating alignment and prediction information to identify potential serotype-specific epitopes. We excluded epitopes that were similarly present in the yellow fever and zika viruses\u2019 proteomes. A total of 15 polymorphic and nine conserved peptides among DENV serotypes were selected. Peptides were spotted on cellulose membranes and tested against sera from rabbits that were monoinfected with each DENV serotype. Although serotype-specific peptides failed to recognize any sera, three conserved peptides were recognized by all anti-dengue sera and were included on an ELISA test employing a well-characterized human sera bank. Of the three peptides, one was able to efficiently identify sera from all four DENV serotypes and to discriminate them from Zika virus positive sera.<\/jats:p>","DOI":"10.3390\/v11111079","type":"journal-article","created":{"date-parts":[[2019,11,19]],"date-time":"2019-11-19T11:30:17Z","timestamp":1574163017000},"page":"1079","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Identification of B-Cell Epitopes with Potential to Serologicaly Discrimnate Dengue from Zika Infections"],"prefix":"10.3390","volume":"11","author":[{"given":"Alice F.","family":"Versiani","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Virologia B\u00e1sica e Aplicada, Departamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil"},{"name":"Laborat\u00f3rio de Pesquisa em Virologia, Departamento de Doen\u00e7as Infecciosas e Parasit\u00e1rias, Faculdade de Medicina de S\u00e3o Jos\u00e9 do Rio Preto, S\u00e3o Jos\u00e9 do Rio Preto 15090-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raissa Prado","family":"Rocha","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Virologia B\u00e1sica e Aplicada, Departamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5569-4893","authenticated-orcid":false,"given":"Tiago A. O.","family":"Mendes","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunologia e Gen\u00f4mica de Parasitos, Departamento de Parasitologia, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Glauco C.","family":"Pereira","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Ezequiel Dias, FUNED, Belo Horizonte 30510-010, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jordana Graziella A.","family":"Coelho dos Reis","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Virologia B\u00e1sica e Aplicada, Departamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniella C.","family":"Bartholomeu","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunologia e Gen\u00f4mica de Parasitos, Departamento de Parasitologia, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1416-8694","authenticated-orcid":false,"given":"Fl\u00e1vio G.","family":"da Fonseca","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Virologia B\u00e1sica e Aplicada, Departamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,11,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Brady, O.J., Gething, P.W., Bhatt, S., Messina, J.P., Brownstein, J.S., Hoen, A.G., Moyes, C.L., Farlow, A.W., Scott, T.W., and Hay, S.I. 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