{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:13:23Z","timestamp":1787030003251,"version":"build-2736575974"},"reference-count":49,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2019,12,8]],"date-time":"2019-12-08T00:00:00Z","timestamp":1575763200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004569","name":"Ministerstwo Nauki i Szkolnictwa Wy\u017cszego","doi-asserted-by":"publisher","award":["KNOW2015\/CB\/PRO1\/11"],"award-info":[{"award-number":["KNOW2015\/CB\/PRO1\/11"]}],"id":[{"id":"10.13039\/501100004569","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Porcine circovirus type 2 (PCV2) is a globally spread pathogen controlled with generally highly efficacious vaccination protocols. In order to compare PCV2 detection profiles in farms with different vaccination statuses, serum (359) and fecal pools (351) and oral fluids (209) from four farms that do not vaccinate against PCV2 (NON-VAC) and from 22 farms that do vaccinate (VAC) were tested with quantitative real-time PCR. Additionally, nucleotide sequences of ORF2 of the virus were obtained from selected samples. Three genotypes, PCV2a, PCV2b, and PCV2d, were detected. Significant differences (p &lt; 0.05) in PCV2 prevalence and quantities between the VAC and NON-VAC farms were evident. In five VAC farms, no viremia or shedding in feces was detected. On the other hand, in four VAC farms, the results were very similar to those from NON-VAC farms. No significant difference in PCV2 prevalence in oral fluids was observed between VAC and NON-VAC farms. An examination of viremia can be recommended for the detection of vaccination efficacy issues. The median of the PCV2 viral loads &gt;6.0 log10 copies\/mL in pooled sera from the vaccinated population should be considered a very strong indication that the vaccination protocol needs revision.<\/jats:p>","DOI":"10.3390\/v11121135","type":"journal-article","created":{"date-parts":[[2019,12,9]],"date-time":"2019-12-09T05:54:51Z","timestamp":1575870891000},"page":"1135","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Real-Time PCR Detection Patterns of Porcine Circovirus Type 2 (PCV2) in Polish Farms with Different Statuses of Vaccination against PCV2"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6078-3172","authenticated-orcid":false,"given":"Aleksandra","family":"Wo\u017aniak","sequence":"first","affiliation":[{"name":"Department of Pathology and Veterinary Diagnostics, Institute of Veterinary Medicine, Warsaw University of Life Sciences\u2014SGGW, Nowoursynowska 159C, 02-776 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0001-5395","authenticated-orcid":false,"given":"Dagmara","family":"Mi\u0142ek","sequence":"additional","affiliation":[{"name":"Department of Pathology and Veterinary Diagnostics, Institute of Veterinary Medicine, Warsaw University of Life Sciences\u2014SGGW, Nowoursynowska 159C, 02-776 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0505-5767","authenticated-orcid":false,"given":"Piotr","family":"Matyba","sequence":"additional","affiliation":[{"name":"Department of Large Animal Diseases with Clinic, Institute of Veterinary Medicine, Warsaw University of Life Sciences\u2014SGGW, Nowoursynowska 100, 02-797 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8889-8120","authenticated-orcid":false,"given":"Tomasz","family":"Stadejek","sequence":"additional","affiliation":[{"name":"Department of Pathology and Veterinary Diagnostics, Institute of Veterinary Medicine, Warsaw University of Life Sciences\u2014SGGW, Nowoursynowska 159C, 02-776 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,12,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"39458","DOI":"10.1038\/srep39458","article-title":"Porcine circovirus type 2 (PCV2) evolution before and after the vaccination introduction: A large scale epidemiological study","volume":"6","author":"Franzo","year":"2016","journal-title":"Sci. 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