{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T10:46:52Z","timestamp":1762253212899,"version":"build-2065373602"},"reference-count":55,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2019,12,11]],"date-time":"2019-12-11T00:00:00Z","timestamp":1576022400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100018537","name":"National Science and Technology Major Project of the Ministry of Science and Technology of China","doi-asserted-by":"publisher","award":["2015ZX09102025"],"award-info":[{"award-number":["2015ZX09102025"]}],"id":[{"id":"10.13039\/501100018537","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31802223"],"award-info":[{"award-number":["31802223"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"\u201cThirteen Five-year Plan\u201d for Sci &amp; Tech Research Program of Jilin Education Department of P.R. China","award":["JJKH20190943KJ"],"award-info":[{"award-number":["JJKH20190943KJ"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Sudan virus (SUDV) causes severe lethal hemorrhagic fever in humans and nonhuman primates. The most effective and economical way to protect against Sudan ebolavirus disease is prophylactic vaccination. However, there are no licensed vaccines to prevent SUDV infections. In this study, a bacterium-like particle (BLP)-based vaccine displaying the extracellular domain of the SUDV glycoprotein (eGP) was developed based on a gram-positive enhancer matrix-protein anchor (GEM-PA) surface display system. Expression of the recombinant GEM-displayed eGP (eGP-PA-GEM) was verified by Western blotting and immunofluorescence assays. The SUDV BLPs (SBLPs), which were mixed with Montanide ISA 201VG plus Poly (I:C) combined adjuvant, could induce high SUDV GP-specific IgG titers of up to 1:40,960 and robust virus-neutralizing antibody titers reached 1:460. The SBLP also elicited T-helper 1 (Th1) and T-helper 2 (Th2) cell-mediated immunity. These data indicate that the SBLP subunit vaccine has the potential to be developed into a promising candidate vaccine against SUDV infections.<\/jats:p>","DOI":"10.3390\/v11121149","type":"journal-article","created":{"date-parts":[[2019,12,12]],"date-time":"2019-12-12T03:20:16Z","timestamp":1576120816000},"page":"1149","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["A Novel Bacterium-Like Particle-Based Vaccine Displaying the SUDV Glycoprotein Induces Potent Humoral and Cellular Immune Responses in Mice"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1800-0741","authenticated-orcid":false,"given":"Shengnan","family":"Xu","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China"},{"name":"Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Military Veterinary Research Institute, Academy of Military Medical Sciences, Changchun 130122, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cuicui","family":"Jiao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Military Veterinary Research Institute, Academy of Military Medical Sciences, Changchun 130122, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongli","family":"Jin","sequence":"additional","affiliation":[{"name":"Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Military Veterinary Research Institute, Academy of Military Medical Sciences, Changchun 130122, China"},{"name":"Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wujian","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Jilin 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China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shengnan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Military Veterinary Research Institute, Academy of Military Medical Sciences, Changchun 130122, China"},{"name":"College of Wildlife and Protected Area, Northeast Forestry University, Harbin 150040, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongbin","family":"He","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Resistant Biology of Shandong, Ruminant Diseases Research Center, College of Life Sciences, Shandong Normal University, Jinan 250014, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hang","family":"Chi","sequence":"additional","affiliation":[{"name":"Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Military Veterinary Research Institute, Academy of Military Medical Sciences, Changchun 130122, China"},{"name":"Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Na","family":"Feng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Military Veterinary Research Institute, Academy of Military Medical Sciences, Changchun 130122, China"},{"name":"Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongkun","family":"Zhao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Military Veterinary Research Institute, Academy of Military Medical Sciences, Changchun 130122, China"},{"name":"Jiangsu Co-innovation Center for Prevention and 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