{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T09:58:12Z","timestamp":1775123892000,"version":"3.50.1"},"reference-count":23,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2020,1,20]],"date-time":"2020-01-20T00:00:00Z","timestamp":1579478400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"MIUR PRIN 2015","award":["PRIN 2015C7PCYZ_002"],"award-info":[{"award-number":["PRIN 2015C7PCYZ_002"]}]},{"name":"Assessorato della Programmazione, Bilancio, Credito e Assetto del territorio, della Regione Autonoma della Sardegna\u201d (Italy), Grant: Legge Regionale 7 agosto 2007","award":["CRP1_574"],"award-info":[{"award-number":["CRP1_574"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Orthohantaviruses, previously known as hantaviruses (family Hantaviridae, order Bunyavirales), are emerging zoonoses hosted by different rodent and insectivore species. Orthohantaviruses are transmitted by aerosolized excreta (urine, saliva and feces) of their reservoir hosts. When transmitted to humans, they cause hemorrhagic fever with renal syndrome (HFRS) in Asia and Europe and hantavirus (cardio) pulmonary syndrome (HPS) in the Americas. Clinical studies have shown that early treatments of HFRS patients with ribavirin (RBV) improve prognosis. Nevertheless, there is the need for urgent development of specific antiviral drugs. In the search for new RNA virus inhibitors, we recently identified a series of variously substituted 5,6-dichloro-1(2)-phenyl-1(2)H-benzo[d][1,2,3]triazole derivatives active against the human respiratory syncytial virus (HRSV). Interestingly, several 2-phenyl-benzotriazoles resulted in fairly potent inhibitors of the Hantaan virus in a chemiluminescence focus reduction assay (C-FRA) showing an EC50 = 4\u20135 \u00b5M, ten-fold more active than ribavirin. Currently, there are no FDA approved drugs for the treatment of orthohantavirus infections. Antiviral activities and cytotoxicity profiles suggest that 5,6-dichloro-1(2)-phenyl-1(2)H-benzo[d][1,2,3]triazoles could be promising candidates for further investigation as a potential treatment of hantaviral diseases.<\/jats:p>","DOI":"10.3390\/v12010122","type":"journal-article","created":{"date-parts":[[2020,1,20]],"date-time":"2020-01-20T04:27:09Z","timestamp":1579494429000},"page":"122","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["5,6-Dichloro-2-Phenyl-Benzotriazoles: New Potent Inhibitors of Orthohantavirus"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3999-4263","authenticated-orcid":false,"given":"Giuseppina","family":"Sanna","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences, University of Cagliari, Cittadella Universitaria, 09042 Monserrato, Cagliari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"Piras","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Pharmacy, University of Sassari, Via Muroni 23\/A, 07100 Sassari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7005-8329","authenticated-orcid":false,"given":"Silvia","family":"Madeddu","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, University of Cagliari, Cittadella Universitaria, 09042 Monserrato, Cagliari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernardetta","family":"Busonera","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, University of Cagliari, Cittadella Universitaria, 09042 Monserrato, Cagliari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Boris","family":"Klempa","sequence":"additional","affiliation":[{"name":"Institute of Virology, Biomedical Research Center Slovak Academy of Sciences, 845 05 Bratislava, Slovakia"},{"name":"Institute of Virology, Helmut-Ruska-Haus, Charit\u00e9 School of Medicine, 10117 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola","family":"Corona","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Pharmacy, University of Sassari, Via Muroni 23\/A, 07100 Sassari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3332-3845","authenticated-orcid":false,"given":"Roberta","family":"Ibba","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Pharmacy, University of Sassari, Via Muroni 23\/A, 07100 Sassari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2791-9178","authenticated-orcid":false,"given":"Gabriele","family":"Murineddu","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Pharmacy, University of Sassari, Via Muroni 23\/A, 07100 Sassari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Carta","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Pharmacy, University of Sassari, Via Muroni 23\/A, 07100 Sassari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberta","family":"Loddo","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, University of Cagliari, Cittadella Universitaria, 09042 Monserrato, Cagliari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,1,20]]},"reference":[{"key":"ref_1","unstructured":"Knipe, D.M., Howley, P.M., Griffin, D.E., Lamb, R.A., Martin, M.A., Roizman, B., and Straus, S.E. 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