{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T22:31:15Z","timestamp":1792017075751,"version":"4.4.0"},"reference-count":35,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2020,2,27]],"date-time":"2020-02-27T00:00:00Z","timestamp":1582761600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100018537","name":"National Science and Technology Major Project of the Ministry of Science and Technology of China","doi-asserted-by":"publisher","award":["2018ZX10101003-005-007"],"award-info":[{"award-number":["2018ZX10101003-005-007"]}],"id":[{"id":"10.13039\/501100018537","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>The Rift Valley fever virus (RVFV) is an arthropod-borne virus that can not only cause severe disease in domestic animals but also in humans. However, the licensed vaccines or available therapeutics for humans do not exist. Here, we report two Gn-specific neutralizing antibodies (NAbs), isolated from a rhesus monkey immunized with recombinant human adenoviruses type 4 expressing Rift Valley fever virus Gn and Gc protein (rHAdV4-GnGcopt). The two NAbs were both able to protect host cells from RVFV infection. The interactions between NAbs and Gn were then characterized to demonstrate that these two NAbs might preclude RVFV glycoprotein rearrangement, hindering the exposure of fusion loops in Gc to endosomal membranes after the virus invades the host cell. The target region for the two NAbs is located in the Gn domain III, implying that Gn is a desired target for developing vaccines and neutralizing antibodies against RVFV.<\/jats:p>","DOI":"10.3390\/v12030259","type":"journal-article","created":{"date-parts":[[2020,2,28]],"date-time":"2020-02-28T09:30:36Z","timestamp":1582882236000},"page":"259","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Characterization of Two Neutralizing Antibodies against Rift Valley Fever Virus Gn Protein"],"prefix":"10.3390","volume":"12","author":[{"given":"Meng","family":"Hao","sequence":"first","affiliation":[{"name":"Comparative Medicine Center, Peking Union Medical College (PUMC) and Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS), Beijing 100021, China"},{"name":"Beijing Institute of Biotechnology, Beijing 100071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Beijing Institute of Biotechnology, Beijing 100071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengnan","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Wildlife and Protected Areas, Northeast Forestry University, Harbin 150040, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengshan","family":"Chen","sequence":"additional","affiliation":[{"name":"Tianjin Institue of Envionmental and Operational Medicine, Key Laboratory of Risk Assessment and Control for Environment and Food Safety, Tianjin 300050, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangyang","family":"Chi","sequence":"additional","affiliation":[{"name":"Beijing Institute of Biotechnology, Beijing 100071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunzhu","family":"Dong","sequence":"additional","affiliation":[{"name":"Beijing Institute of Biotechnology, Beijing 100071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengfei","family":"Fan","sequence":"additional","affiliation":[{"name":"Beijing Institute of Biotechnology, Beijing 100071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujiao","family":"Liu","sequence":"additional","affiliation":[{"name":"Beijing Institute of Biotechnology, Beijing 100071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Chen","sequence":"additional","affiliation":[{"name":"Beijing Institute of Biotechnology, Beijing 100071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohong","family":"Song","sequence":"additional","affiliation":[{"name":"Beijing Institute of Biotechnology, Beijing 100071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuling","family":"Liu","sequence":"additional","affiliation":[{"name":"Beijing Institute of Biotechnology, Beijing 100071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changming","family":"Yu","sequence":"additional","affiliation":[{"name":"Beijing Institute of Biotechnology, Beijing 100071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianmin","family":"Li","sequence":"additional","affiliation":[{"name":"Beijing Institute of Biotechnology, Beijing 100071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianzhu","family":"Xia","sequence":"additional","affiliation":[{"name":"Comparative Medicine Center, Peking Union Medical College (PUMC) and Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS), Beijing 100021, China"},{"name":"Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun 130000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,2,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"651","DOI":"10.2217\/fvl-2017-0060","article-title":"Recent advances in the development of antiviral therapeutics for Rift Valley fever virus infection","volume":"12","author":"Atkins","year":"2017","journal-title":"Future Virol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1017\/S0950268810001020","article-title":"Rift Valley fever in Kenya: History of epizootics and identification of vulnerable districts","volume":"139","author":"Murithi","year":"2011","journal-title":"Epidemiol. 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