{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T11:17:05Z","timestamp":1770895025955,"version":"3.50.1"},"reference-count":47,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2020,6,12]],"date-time":"2020-06-12T00:00:00Z","timestamp":1591920000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF-2018R1A5A1025077"],"award-info":[{"award-number":["NRF-2018R1A5A1025077"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF-2019R1C1C1004576"],"award-info":[{"award-number":["NRF-2019R1C1C1004576"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>The immune-suppressive effects of omega-3 (n-3) polyunsaturated fatty acids (PUFAs) on T cells have been observed via multiple in vitro and in vivo models. However, the precise mechanism that causes these effects is still undefined. In this study, we investigated whether n-3 PUFAs regulated T cell receptor (TCR) and peptide-major histocompatibility complex (pMHC) interactions. The expansion of anti-viral CD8+ T cells that endogenously synthesize n-3 PUFAs (FAT-1) dramatically decreased upon lymphocytic choriomeningitis virus (LCMV) infection in vivo. This decrease was not caused by the considerable reduction of TCR expression or the impaired chemotactic activity of T cells. Interestingly, a highly inclined and laminated optical sheet (HILO) microscopic analysis revealed that the TCR motility was notably reduced on the surface of the FAT-1 CD8+ T cells compared to the wild type (WT) CD8+ T cells. Importantly, the adhesion strength of the FAT-1 CD8+ T cells to the peptide-MHC was significantly lower than that of the WT CD8+T cells. Consistent with this result, treatment with docosahexaenoic acid (DHA), one type of n-3 PUFA, significantly decreased CD8+ T cell adhesion to the pMHC. Collectively, our results reveal a novel mechanism through which n-3 PUFAs decrease TCR-pMHC interactions by modulating TCR mobility on CD8+ T cell surfaces.<\/jats:p>","DOI":"10.3390\/v12060639","type":"journal-article","created":{"date-parts":[[2020,6,12]],"date-time":"2020-06-12T05:02:24Z","timestamp":1591938144000},"page":"639","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["n-3 Polyunsaturated Fatty Acids Impede the TCR Mobility and the TCR\u2013pMHC Interaction of Anti-Viral CD8+ T Cells"],"prefix":"10.3390","volume":"12","author":[{"given":"Younghyun","family":"Lim","sequence":"first","affiliation":[{"name":"Department of Life Science, Chung-Ang University, Seoul 06974, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seyoung","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Life Science, Chung-Ang University, Seoul 06974, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sehoon","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Chung-Ang University, Seoul 06974, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong-In","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Life Science, Chung-Ang University, Seoul 06974, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyung Won","family":"Kang","sequence":"additional","affiliation":[{"name":"Division of Biotechnology, College of Environmental and Bioresources, Jeonbuk National University, Iksan 54596, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"So-Hee","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, the Catholic University of Korea, Bucheon 14662, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sang-Myeong","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Biotechnology, College of Environmental and Bioresources, Jeonbuk National University, Iksan 54596, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hye Ran","family":"Koh","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Chung-Ang University, Seoul 06974, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young-Jin","family":"Seo","sequence":"additional","affiliation":[{"name":"Department of Life Science, Chung-Ang University, Seoul 06974, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,6,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"S59","DOI":"10.1079\/BJN2001457","article-title":"Regulatory potential of n-3 fatty acids in immunological and inflammatory processes","volume":"87","author":"Grimm","year":"2002","journal-title":"Br. 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