{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T23:21:14Z","timestamp":1781911274006,"version":"3.54.5"},"reference-count":26,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2020,6,24]],"date-time":"2020-06-24T00:00:00Z","timestamp":1592956800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008771","name":"Animal and Plant Quarantine Agency","doi-asserted-by":"publisher","award":["B-1543083-2019-21-02"],"award-info":[{"award-number":["B-1543083-2019-21-02"]}],"id":[{"id":"10.13039\/501100008771","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Atypical porcine pestivirus (APPV), currently classified as pestivirus K, causes congenital tremor (CT) type A-II in piglets. Eighteen APPV strains were identified from 2297 South Korean wild boars captured in 2019. Phylogenetic analysis of the structural protein E2 and nonstructural proteins NS3 and Npro classified the APPV viruses, including reference strains, into Clades I, II and III. Clade I was divided into four subclades; however, the strains belonging to the four subclades differed slightly, depending on the tree analysis, the NS3, E2, and Npro genes. The maximum-likelihood method was assigned to South Korean wild boar APPV strains to various subclades within the three trees: subclades I.1 and I.2 in the E2 tree, subclade I.1 in the Npro tree, and subclades I.1 and I.4 in the NS3 ML tree. In conclusion, APPV among South Korean wild boars belonging to Clade I may be circulating at a higher level than among the South Korean domestic pig populations.<\/jats:p>","DOI":"10.3390\/v12060680","type":"journal-article","created":{"date-parts":[[2020,6,24]],"date-time":"2020-06-24T08:54:50Z","timestamp":1592988890000},"page":"680","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Prevalence and Genetic Diversity of Atypical Porcine Pestivirus (APPV) Detected in South Korean Wild Boars"],"prefix":"10.3390","volume":"12","author":[{"given":"SeEun","family":"Choe","sequence":"first","affiliation":[{"name":"Virus Disease Division, Animal and Plant Quarantine Agency, Gimchen, Gyeongbuk-do 39660, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gyu-Nam","family":"Park","sequence":"additional","affiliation":[{"name":"Virus Disease Division, Animal and Plant Quarantine Agency, Gimchen, Gyeongbuk-do 39660, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ra Mi","family":"Cha","sequence":"additional","affiliation":[{"name":"Virus Disease Division, Animal and Plant Quarantine Agency, Gimchen, Gyeongbuk-do 39660, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bang-Hun","family":"Hyun","sequence":"additional","affiliation":[{"name":"Virus Disease Division, Animal and Plant Quarantine Agency, Gimchen, Gyeongbuk-do 39660, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bong-Kyun","family":"Park","sequence":"additional","affiliation":[{"name":"Virus Disease Division, Animal and Plant Quarantine Agency, Gimchen, Gyeongbuk-do 39660, Korea"},{"name":"College of Veterinary Medicine, Seoul University, Gwanak-ro, Gwanak-gu, Seoul 08826, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong-Jun","family":"An","sequence":"additional","affiliation":[{"name":"Virus Disease Division, Animal and Plant Quarantine Agency, Gimchen, Gyeongbuk-do 39660, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,6,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2016","DOI":"10.1099\/jgv.0.000873","article-title":"Proposed revision to the taxonomy of the genus Pestivirus, family Flaviviridae","volume":"98","author":"Smith","year":"2017","journal-title":"J. Gen. Virol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2994","DOI":"10.1099\/jgv.0.000251","article-title":"Discovery of a novel putative atypical porcine pestivirus in pigs in the United States","volume":"96","author":"Hause","year":"2015","journal-title":"J. Gen. Virol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"862","DOI":"10.3389\/fmicb.2017.00862","article-title":"Atypical porcine pestivirus as a novel type of pestivirus in pigs in China","volume":"8","author":"Yuan","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"e22","DOI":"10.1111\/tbed.12532","article-title":"High prevalence of highly variable atypical porcine pestiviruses found in Germany","volume":"64","author":"Beer","year":"2016","journal-title":"Transboud. Emerg. Dis."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"De Groof, A., Deijs, M., Guelen, K., van Grinsven, L., van Os-Galdos, L., Vogels, W., and van der Hoek, L. (2016). Atypical porcine pestivirus: A possible cause of congenital tremor type A-II in newborn piglets. Viruses, 8.","DOI":"10.3390\/v8100271"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"27735","DOI":"10.1038\/srep27735","article-title":"Presence of atypical porcine pestivirus (APPV) genome in newborn piglets correlates with congenital tremor","volume":"6","author":"Postel","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13567-016-0406-1","article-title":"Congenital infection with atypical porcine pestivirus (APPV) is associated with disease and viral persistence","volume":"48","author":"Schwarz","year":"2017","journal-title":"Vet. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1111\/tbed.12824","article-title":"Detection of atypical porcine pestivirus in Brazil in the central nervous system of suckling piglets with congenital tremor","volume":"65","author":"Gatto","year":"2018","journal-title":"Transboud Emerg. Dis."},{"key":"ref_9","first-page":"209","article-title":"Detection of atypical porcine pestivirus (APPV) from a case of congenital tremor in Korea","volume":"40","author":"Kim","year":"2017","journal-title":"Korean. J. Vet. Sev."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1142","DOI":"10.3390\/v11121142","article-title":"Molecular Survey and Phylogenetic Analysis of Atypical Porcine Pestivirus (APPV) Identification in Swine and Wild Boar from Northern Italy","volume":"11","author":"Enrica","year":"2019","journal-title":"Viruses."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2104","DOI":"10.3201\/eid2312.170951","article-title":"High Abundance and Genetic Variability of Atypical Porcine Pestivirus in Pigs from Europe and Asia","volume":"23","author":"Postel","year":"2017","journal-title":"Emerg. Infect. Dis."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1087","DOI":"10.1111\/tbed.12854","article-title":"Frequent infection of wild boar with atypical porcine pestivirus (APPV)","volume":"65","author":"Cagatay","year":"2018","journal-title":"Transboud Emerg. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1136\/vr.104824","article-title":"Atypical porcine pestivirus in wild boar (Sus Scrofa), Spain","volume":"183","author":"Ganges","year":"2018","journal-title":"Vet. Rec."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2109","DOI":"10.1016\/j.meegid.2014.12.032","article-title":"Homomolgours recombination in pestiviruses: Identification of three putative novel events between different subtypes\/genogroups","volume":"30","author":"Weber","year":"2015","journal-title":"Infect. Genet. Evol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/S0166-0934(98)00109-8","article-title":"Application of a computer program for genetic typing of classical swine fever virus isolates from Germany","volume":"75","author":"Depner","year":"1998","journal-title":"J. Virol. Methods."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"e202","DOI":"10.1111\/tbed.12675","article-title":"Phylogenetic and genomic characterization of a novel atypical porcine pestivirus in China","volume":"65","author":"Zhang","year":"2018","journal-title":"Transboud Emerg. Dis."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1099\/jgv.0.001188","article-title":"Prevalence and genome characteristics of atypical porcine pestivirus in Southwest China","volume":"100","author":"Zhou","year":"2019","journal-title":"J. Gen. Virol."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Choe, S., Kim, J.H., Kim, K.S., Song, S., Kang, W.C., Kim, H.J., Park, G.N., Cha, R.M., Cho, I.S., and Hyun, B.H. (2019). Impact of a live attenuated classical swine fever virus introduced onto Jeju Island, a CSF-free area. Pathogens., 8.","DOI":"10.3390\/pathogens8040251"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"4876","DOI":"10.1093\/nar\/25.24.4876","article-title":"The CLUSTAL_X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools","volume":"25","author":"Thompson","year":"1997","journal-title":"Nucleic Acids Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1870","DOI":"10.1093\/molbev\/msw054","article-title":"MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets","volume":"33","author":"Kumar","year":"2016","journal-title":"Mol. Biol. Evol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1645","DOI":"10.1111\/tbed.12699","article-title":"First report of the novel atypical porcine pestivirus in Spain and a retrospective study","volume":"64","author":"Canturri","year":"2017","journal-title":"Transbound Emerg. Dis."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Kaufmann, C., Stalder, H., Sidler, X., Renzullo, S., Gurtner, C., Grahofer, A., and Schweizer, M. (2019). Long-Term Circulation of Atypical Porcine Pestivirus (APPV) within Switzerland. Viruses., 11.","DOI":"10.3390\/v11070653"},{"key":"ref_23","first-page":"406","article-title":"The neighbor-joining method: A new method for reconstructing phylogenetic trees","volume":"4","author":"Saitou","year":"1987","journal-title":"Mol. Biol. Evol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"35","DOI":"10.3389\/fvets.2019.00035","article-title":"Atypical porcine pestivirus (APPV) as a New Species of Pestivirus in Pig Production","volume":"6","author":"Gatto","year":"2019","journal-title":"Front. Vet. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"104259","DOI":"10.1016\/j.meegid.2020.104259","article-title":"Genetic characterization and recombination analysis of atypical porcine pestivirus","volume":"81","author":"Guo","year":"2020","journal-title":"Infect. Genet. Evol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.virol.2019.04.010","article-title":"12 novel atypical porcine pestivirus genomes from neonatal piglets with congenital tremors: A newly emerging branch and high prevalence in China","volume":"533","author":"Yan","year":"2019","journal-title":"Virology."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/12\/6\/680\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:42:22Z","timestamp":1760175742000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/12\/6\/680"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,24]]},"references-count":26,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2020,6]]}},"alternative-id":["v12060680"],"URL":"https:\/\/doi.org\/10.3390\/v12060680","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,24]]}}}