{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T02:59:58Z","timestamp":1760237998963,"version":"build-2065373602"},"reference-count":59,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2020,7,6]],"date-time":"2020-07-06T00:00:00Z","timestamp":1593993600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Barmah Forest virus (BFV) is a medically important mosquito-borne alphavirus endemic to Australia. Symptomatic disease can be a major cause of morbidity, associated with fever, rash, and debilitating arthralgia. BFV disease is similar to that caused by Ross River virus (RRV), the other major Australian alphavirus. Currently, just four BFV whole-genome sequences are available with no genome-scale phylogeny in existence to robustly characterise genetic diversity. Thirty novel genome sequences were derived for this study, for a final 34-taxon dataset sampled over a 44 year period. Three distinct BFV genotypes were characterised (G1\u20133) that have circulated in Australia and Papua New Guinea (PNG). Evidence of spatio-temporal co-circulation of G2 and G3 within regions of Australia was noted, including in the South West region of Western Australia (WA) during the first reported disease outbreaks in the state\u2019s history. Compared with RRV, the BFV population appeared more stable with less frequent emergence of novel lineages. Preliminary in vitro assessment of RRV and BFV replication kinetics found that RRV replicates at a significantly faster rate and to a higher, more persistent titre compared with BFV, perhaps indicating mosquitoes may be infectious with RRV for longer than with BFV. This investigation resolved a greater diversity of BFV, and a greater understanding of the evolutionary dynamics and history was attained.<\/jats:p>","DOI":"10.3390\/v12070732","type":"journal-article","created":{"date-parts":[[2020,7,6]],"date-time":"2020-07-06T11:07:42Z","timestamp":1594033662000},"page":"732","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Phylogenetic and Timescale Analysis of Barmah Forest Virus as Inferred from Genome Sequence Analysis"],"prefix":"10.3390","volume":"12","author":[{"given":"Alice","family":"Michie","sequence":"first","affiliation":[{"name":"School of Biomedical Sciences, University of Western Australia, Nedlands, WA 6009, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timo","family":"Ernst","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, University of Western Australia, Nedlands, WA 6009, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I-Ly Joanna","family":"Chua","sequence":"additional","affiliation":[{"name":"PathWest Laboratory Medicine Western Australia, Perth, WA 6000, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael D. A.","family":"Lindsay","sequence":"additional","affiliation":[{"name":"Environmental Health Hazards, Department of Health, Perth, WA 6000, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter J.","family":"Neville","sequence":"additional","affiliation":[{"name":"Environmental Health Hazards, Department of Health, Perth, WA 6000, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jay","family":"Nicholson","sequence":"additional","affiliation":[{"name":"Environmental Health Hazards, Department of Health, Perth, WA 6000, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Jardine","sequence":"additional","affiliation":[{"name":"Environmental Health Hazards, Department of Health, Perth, WA 6000, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5918-2190","authenticated-orcid":false,"given":"John S.","family":"Mackenzie","sequence":"additional","affiliation":[{"name":"PathWest Laboratory Medicine Western Australia, Perth, WA 6000, Australia"},{"name":"Faculty of Health Sciences, Curtin University, Bentley WA 6102, Australia"},{"name":"School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia 4067, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3107-5182","authenticated-orcid":false,"given":"David W.","family":"Smith","sequence":"additional","affiliation":[{"name":"PathWest Laboratory Medicine Western Australia, Perth, WA 6000, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4715-6662","authenticated-orcid":false,"given":"Allison","family":"Imrie","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, University of Western Australia, Nedlands, WA 6009, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,7,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Knope, K., Doggett, S.L., Jansen, C.C., Johansen, C.A., Kurucz, N., Feldman, R., Lynch, S.E., Hobby, M.P., Sly, A., and Jardine, A. (2019). Arboviral diseases and malaria in Australia, 2014\u20132015: Annual report of the National Arbovirus and Malaria Advisory Committee. Commun. Dis. Intell., 43.","DOI":"10.33321\/cdi.2019.43.14"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"159","DOI":"10.5694\/j.1326-5377.1998.tb116019.x","article-title":"A comparison of the diseases caused by Ross River virus and Barmah Forest virus","volume":"169","author":"Flexman","year":"1998","journal-title":"Med. J. Aust."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1038\/icb.1979.52","article-title":"Isolation of Virus Strains from Mosquitoes Collected in Queensland 1972\u20131976","volume":"57","author":"Doherty","year":"1979","journal-title":"Aust. J. Exp. Biol. Med. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1038\/icb.1982.51","article-title":"Viruses Recovered from Mosquitoes and Wildlife Serum Collected in the Murray Valley of South-Eastern Australia, February 1974, During and Epidemic of Encephalitis","volume":"60","author":"Marshall","year":"1982","journal-title":"Aust. J. Exp. Biol. Med. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"146","DOI":"10.5694\/j.1326-5377.1988.tb112780.x","article-title":"Illness caused by a Barmah Forest-like virus in New South Wales","volume":"148","author":"Boughton","year":"1988","journal-title":"Med. J. Aust."},{"key":"ref_6","first-page":"110","article-title":"A concurrent outbreak of Barmah Forest and Ross River virus disease in Nhulunbuy, Northern Territory","volume":"16","author":"Merianos","year":"1992","journal-title":"Commun. Dis. Intell."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"22","DOI":"10.3201\/eid0101.950104","article-title":"Emergence of Barmah Forest Virus in Western Australia","volume":"1","author":"Lindsay","year":"1995","journal-title":"Emerg. Infect. Dis."},{"key":"ref_8","first-page":"235","article-title":"Mosquitoes and Barmah Forest Virus: The 1995 Epidemic in New South Wales","volume":"7","author":"Russell","year":"1997","journal-title":"Arbovirus Res. Aust."},{"key":"ref_9","first-page":"600","article-title":"An outbreak of Barmah Forest virus disease in Victoria","volume":"26","author":"Passmore","year":"2002","journal-title":"Commun. Dis. Intell."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2266","DOI":"10.3201\/eid2512.191070","article-title":"Divergent Barmah Forest Virus from Papua New Guinea","volume":"25","author":"Caly","year":"2019","journal-title":"Emerg. Infect. Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"955","DOI":"10.1016\/S0020-7519(98)00053-8","article-title":"Mosquito-borne alphaviruses in Australia: The current scene and implication of climate change for human health","volume":"28","author":"Russell","year":"1998","journal-title":"Int. J. Parasitol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"63","DOI":"10.5365\/wpsar.2012.3.1.004","article-title":"Surveillance should be strengthened to improve epidemiological understandings of mosquito-borne Barmah Forest virus infection","volume":"3","author":"Ehlkes","year":"2012","journal-title":"West. Pac. Surveill. Response J."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1440-6055.2008.00677.x","article-title":"Mosquito-borne disease and climate change in Australia: Time for a reality check","volume":"48","author":"Russell","year":"2009","journal-title":"Aust. J. Entomol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1089\/vbz.2007.0152","article-title":"Ross River virus and Barmah Forest virus infections: A review of history, ecology, and predictive models, with implications for tropical northern Australia","volume":"8","author":"Jacups","year":"2008","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"291","DOI":"10.5694\/j.1326-5377.1995.tb139902.x","article-title":"An Outbreak of Barmah Forest Virus Disease in the South-West of Western Australia","volume":"162","author":"Lindsay","year":"1995","journal-title":"Med. J. Aust."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1289\/ehp.8568","article-title":"Weather Variability, Tides and Barmah Forest Virus Disease in the Gladstone Region, Australia","volume":"114","author":"Naish","year":"2006","journal-title":"Environ. Health Perspect."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1146\/annurev.ento.47.091201.145100","article-title":"Ross River Virus: Ecology and Distribution","volume":"47","author":"Russell","year":"2002","journal-title":"Annu. Rev. Entomol."},{"key":"ref_18","unstructured":"Doggett, S.L., and Russell, R.C. (1996, January 25\u201329). Aedes notoscriptus can transmit inland and coastal isolates of Ross River virus and Barmah Forest viruses from New South Wales. Proceedings of the Arbovirus Research, Surfers Paradise, Australia."},{"key":"ref_19","first-page":"14","article-title":"Solving the urban puzzle of Ross River and Barmah Forest viruses","volume":"8","author":"Boyd","year":"2001","journal-title":"Arbovirus Res. Aust."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2729","DOI":"10.1128\/JVI.05591-11","article-title":"Genome-Scale Phylogeny of the Alphavirus Genus Suggests a Marine Origin","volume":"86","author":"Forrester","year":"2012","journal-title":"J. Virol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.tmaid.2010.05.005","article-title":"The viruses of Australia and risks to tourists","volume":"9","author":"Smith","year":"2011","journal-title":"Travel Med. Infect. Dis."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1016\/j.virusres.2011.06.021","article-title":"Genetic Characterisation of K13965, a strain of Oak Vale virus from Western Australia","volume":"160","author":"Quan","year":"2011","journal-title":"Virus Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"230","DOI":"10.4269\/ajtmh.1997.57.230","article-title":"Genetic Stability Among Temporally and Geographically Diverse Isolates of Barmah Forest Virus","volume":"57","author":"Poidinger","year":"1997","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Michie, A., Dhanasekaran, V., Lindsay, M.D.A., Neville, P.J., Nicholson, J., Jardine, A., Mackenzie, J.S., Smith, D.W., and Imrie, A. (2020). Genome-Scale Phylogeny and Evolutionary Analysis of Ross River Virus Reveals Periodic Sweeps of Lineage Dominance in Western Australia, 1977\u20132014. J. Virol., 94.","DOI":"10.1128\/JVI.01234-19"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Forrester, N.L., Wertheim, J.O., Dugan, V.G., Auguste, A.J., Lin, D., Adams, A.P., Chen, R., Gorchakov, R., Leal, G., and Estrada-Franco, J.G. (2017). Evolution and spread of Venezuelan equine encephalitis complex alphavirus in the Americas. PLoS Negl. Trop. Dis., 11.","DOI":"10.1371\/journal.pntd.0005693"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e00620-19","DOI":"10.1128\/JVI.00620-19","article-title":"Introduction and Dispersal of Sindbis Virus from Central Africa to Europe","volume":"93","author":"Ling","year":"2019","journal-title":"J. Virol."},{"key":"ref_27","unstructured":"Andrews, S., Lindenbaum, P., Howard, B., and Ewels, P. (2019, December 31). FastQC. Available online: https:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc\/."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1093\/molbev\/mst010","article-title":"MAFFT Multiple Sequences Alignment Software Version 7: Improvements in Performance and Usability","volume":"30","author":"Katoh","year":"2013","journal-title":"Mol. Biol. Evol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1312","DOI":"10.1093\/bioinformatics\/btu033","article-title":"RAxML version 8: A tool for phylogenetic analysis and post-analysis of large phylogenies","volume":"30","author":"Stamatakis","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1038\/nmeth.2109","article-title":"jModelTest2: More models, new heuristics and parallel computing","volume":"9","author":"Darriba","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_31","unstructured":"Rambaut, A. (2019, December 31). Figtree 1.4.4. Available online: http:\/\/tree.bio.ed.ac.uk\/software\/figtree\/."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"vew007","DOI":"10.1093\/ve\/vew007","article-title":"Exploring the temporal structure of heterochonous sequences using TempEst (formerly Path-O-Gen)","volume":"2","author":"Rambaut","year":"2016","journal-title":"Virus Evol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"vey016","DOI":"10.1093\/ve\/vey016","article-title":"Bayesian phylogenetic and phylodynamic data integration using BEAST 1.10","volume":"4","author":"Suchard","year":"2018","journal-title":"Virus Evol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1093\/sysbio\/syy032","article-title":"Posterior summarisation in Bayesian phylogenetics using Tracer 1.7","volume":"67","author":"Rambaut","year":"2018","journal-title":"Syst. Biol."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Rodrigo, A.G., and Learn, G.H. (2000). HIV Signature and Sequence Variation Analysis. Computational Analysis of HIV Molecular Sequences, Kluwer Academic Publishers.","DOI":"10.1007\/b112102"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1208","DOI":"10.1093\/molbev\/msi105","article-title":"Not So Different After All: A Comparison of Methods for Detecting Amino Acid Sites Under Selection","volume":"22","author":"Frost","year":"2005","journal-title":"Mol. Biol. Evol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1196","DOI":"10.1093\/molbev\/mst030","article-title":"FUBAR: A Fast, Unconstrained Bayesian AppRoximation for Inferring Selection","volume":"30","author":"Murrell","year":"2013","journal-title":"Mol. Biol. Evol."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Murrell, B., Wertheim, J.O., Moola, S., Weighill, T., Scheffler, K., and Kosakovsky Pond, S.L. (2012). Detecting Individual Sites Subject to Episodic Diversifying Selection. PLoS Genet., 8.","DOI":"10.1371\/journal.pgen.1002764"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1093\/bioinformatics\/16.6.562","article-title":"RDP: Detection of recombination amongst aligned sequences","volume":"16","author":"Martin","year":"2000","journal-title":"Bioinformatics"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"13757","DOI":"10.1073\/pnas.241370698","article-title":"Evaluation of methods for detecting recombination from DNA sequences","volume":"98","author":"Posada","year":"2001","journal-title":"PNAS"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1089\/aid.2005.21.98","article-title":"A modified bootscan algoritm for automated identification of recombinant sequences and recombination breakpoints","volume":"21","author":"Martin","year":"2005","journal-title":"AIDS Res. Hum. Retrovir."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1093\/molbev\/msx263","article-title":"Improved algorithmic complexity for the 3SEQ recombination detection algorithm","volume":"35","author":"Lam","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1006\/viro.1999.0056","article-title":"Possible emergence of new geminiviruses by frequent recombination","volume":"265","author":"Padidam","year":"1999","journal-title":"Virology"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1093\/bioinformatics\/16.7.573","article-title":"Sister-Scanning: A Monte Carlo procedure for assessing signals in recombinant sequences","volume":"16","author":"Gibbs","year":"2000","journal-title":"Bioinformatics"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1093\/oxfordjournals.molbev.a026121","article-title":"Phylogenetic Evidence for Recombination in Dengue Virus","volume":"16","author":"Holmes","year":"1999","journal-title":"Mol. Biol. Evol."},{"key":"ref_46","first-page":"126","article-title":"Analyzing the mosaic structure of genes","volume":"34","year":"1992","journal-title":"J. Mol. Evol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/j.virol.2010.11.025","article-title":"Lineage Replacement accompanying duplication and rapid fixation of an RNA element in the nsP3 gene in a species of alphavirus","volume":"410","author":"Aaskov","year":"2011","journal-title":"Virology"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1093\/nar\/9.1.133","article-title":"Optimal computer folding of large RNA sequences using thermodynamics and auxillary information","volume":"9","author":"Zuker","year":"1981","journal-title":"Nucleic Acids Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"vey012","DOI":"10.1093\/ve\/vey012","article-title":"Chikungunya virus evolution following a large 3\u2019 UTR deletion resutls in host-specific molecular changes in protein-coding regions","volume":"4","author":"Morley","year":"2018","journal-title":"Virus Evol."},{"key":"ref_50","first-page":"1","article-title":"O\u2019nyong nyong Virus Molecular Determinants of Unique Vector Specificity Reside in Non-Structural Protein 3","volume":"7","author":"Ledermann","year":"2013","journal-title":"PLoS Negl. Trop. Dis."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1014","DOI":"10.1128\/JVI.01586-09","article-title":"Evolutionary Patterns of Eastern Equine Encephalitis Virus in North versus South America Suggest Ecological Differences and Taxanomic Revision","volume":"84","author":"Arrigo","year":"2010","journal-title":"J. Virol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"vex010","DOI":"10.1093\/ve\/vex010","article-title":"Understanding the evolution and spread of chikungunya virus in the Americas using complete genome sequences","volume":"3","author":"Sahadeo","year":"2017","journal-title":"Virus Evol."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Tan, Y., Lam, T., Heberlein-Larson, L.A., Smole, S.C., Auguste, A.J., Hennigan, S., Halpin, R.A., Fedorova, N.B., Puri, V., and Stockwell, T.B. (2018). Large-Scale Complete-Genome Sequencing and Phylodynamic Analysis of Eastern Equine Encephalitis Virus Reveals Source-Sink Transmission Dynamics in the United States. J. Virol., 92.","DOI":"10.1128\/JVI.00074-18"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1016\/j.antiviral.2012.04.002","article-title":"Alphaviruses: Population genetics and determinants of emergence","volume":"94","author":"Weaver","year":"2012","journal-title":"Antivir. Res."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"5997","DOI":"10.1073\/pnas.85.16.5997","article-title":"Western Equine Encephalitis virus is a recombinants","volume":"85","author":"Hahn","year":"1988","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1128\/jvi.71.1.613-623.1997","article-title":"Recombinational History and Molecular Evolution of Western Equine Encephalitis Complex Alphaviruses","volume":"71","author":"Weaver","year":"1997","journal-title":"J. Virol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1071\/ZO03042","article-title":"Prevalence of neutralising antibody to Barmah Forest, Sindbis and Trubanaman viruses in animals and humans in the south-west of Western Australia","volume":"53","author":"Johansen","year":"2005","journal-title":"Aust. J. Zool."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Gressitt, J.L. (1982). An ecological check-list of New Guinea Recent mammals. Biogeography and Ecology of New Guinea, Springer.","DOI":"10.1007\/978-94-009-8632-9"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1071\/MU03026","article-title":"The Australo-Papuan bird migration system: Another consequence of Wallace\u2019s Line","volume":"104","author":"Dingle","year":"2004","journal-title":"Emu\u2013Austral Ornithol."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/12\/7\/732\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:48:03Z","timestamp":1760176083000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/12\/7\/732"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,6]]},"references-count":59,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2020,7]]}},"alternative-id":["v12070732"],"URL":"https:\/\/doi.org\/10.3390\/v12070732","relation":{},"ISSN":["1999-4915"],"issn-type":[{"type":"electronic","value":"1999-4915"}],"subject":[],"published":{"date-parts":[[2020,7,6]]}}}