{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T12:59:14Z","timestamp":1772801954583,"version":"3.50.1"},"reference-count":69,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2020,8,14]],"date-time":"2020-08-14T00:00:00Z","timestamp":1597363200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Foundation for the Development of Scientific and Technological Actions and the Research of the State of Rond\u00f4nia - FAPERO and National Institute of Epidemiology of Western Amazonia\u2014INCT EpiAmO.","award":["Process: 011331000510000.02\/2018 Tender: SUMMONS 001\/2018 PPSUS"],"award-info":[{"award-number":["Process: 011331000510000.02\/2018 Tender: SUMMONS 001\/2018 PPSUS"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>The purpose of the study was to classify, through phylogenetic analyses, the main arboviruses that have been isolated in the metropolitan region of Porto Velho, Rond\u00f4nia, Brazil. Serum samples from patients with symptoms suggesting arboviruses were collected and tested by One Step RT-qPCR for Zika, Dengue (serotypes 1\u20134), Chikungunya, Mayaro and Oropouche viruses. Positive samples were amplified by conventional PCR and sequenced utilizing the Sanger method. The obtained sequences were aligned, and an evolutionary analysis was carried out using Bayesian inference. A total of 308 samples were tested. Of this total, 20 had a detectable viral load for Dengue, being detected DENV1 (18\/20), co-infection DENV1 and DENV2 (1\/20) and DENV4 (1\/20). For Dengue serotype 3 and for the CHIKV, ZIKV, MAYV and OROV viruses, no individuals with a detectable viral load were found. A total of 9 of these samples were magnified by conventional PCR for sequencing. Of these, 6 were successfully sequenced and, according to the evolutionary profile, 5 corresponded to serotype DENV-1 genotype V, and 1 to serotype DENV-4 genotype II. In the study, we demonstrate co-circulation of the DENV-1 genotype V and the DENV-4 genotype II. Co-circulation of several DENV serotypes in the same city poses a risk to the population and is correlated with the increase of the most severe forms of the disease. Similarly, co-circulation of genetically distinct DENV and the occurrence of simultaneous infections can affect recombination events and lead to the emergence of more virulent isolates.<\/jats:p>","DOI":"10.3390\/v12080889","type":"journal-article","created":{"date-parts":[[2020,8,14]],"date-time":"2020-08-14T08:28:35Z","timestamp":1597393715000},"page":"889","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Phylogenetic Characterization of Arboviruses in Patients Suffering from Acute Fever in Rond\u00f4nia, Brazil"],"prefix":"10.3390","volume":"12","author":[{"given":"Jackson Alves da Silva","family":"Queiroz","sequence":"first","affiliation":[{"name":"Oswaldo Cruz Foundation of Rond\u00f4nia\u2014FIOCRUZ\/RO, Porto Velho RO 76812 245, Rond\u00f4nia, Brazil"},{"name":"Postgraduate Program in Experimental Biology of Federal University of Rond\u00f4nia\u2014PGBIOEXP, Porto Velho RO 76801 059, Rond\u00f4nia, Brazil"},{"name":"National Institute of Epidemiology of Western Amazonia\u2014INCT EpiAmO, Porto Velho RO 76812 245, Rond\u00f4nia, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3522-3306","authenticated-orcid":false,"given":"Luan Felipo","family":"Botelho-Souza","sequence":"additional","affiliation":[{"name":"Oswaldo Cruz Foundation of Rond\u00f4nia\u2014FIOCRUZ\/RO, Porto Velho RO 76812 245, Rond\u00f4nia, Brazil"},{"name":"National Institute of Epidemiology of Western Amazonia\u2014INCT EpiAmO, Porto Velho RO 76812 245, Rond\u00f4nia, Brazil"},{"name":"Apar\u00edcio Carvalho University Center, Porto Velho RO 76811-678, Rond\u00f4nia, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felipe Souza","family":"Nogueira-Lima","sequence":"additional","affiliation":[{"name":"Oswaldo Cruz Foundation of Rond\u00f4nia\u2014FIOCRUZ\/RO, Porto Velho RO 76812 245, Rond\u00f4nia, Brazil"},{"name":"Postgraduate Program 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Ribas","family":"Zambenedetti","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology of Paran\u00e1 -IBMP, Curitiba PR 81350-010, Rond\u00f4nia, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabricio Klerinton","family":"Marchini","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology of Paran\u00e1 -IBMP, Curitiba PR 81350-010, Rond\u00f4nia, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivo Alberto","family":"Borghetti","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology of Paran\u00e1 -IBMP, Curitiba PR 81350-010, Rond\u00f4nia, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7761-5498","authenticated-orcid":false,"given":"Dhelio Batista","family":"Pereira","sequence":"additional","affiliation":[{"name":"Tropical Medicine of Rond\u00f4nia Center Research\u2014CEPEM\/RO, Porto Velho RO 76812 329, 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