{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T00:41:47Z","timestamp":1790469707379,"version":"4.1.0"},"reference-count":59,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2020,8,14]],"date-time":"2020-08-14T00:00:00Z","timestamp":1597363200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004488","name":"Croatian Science Foundation","doi-asserted-by":"publisher","award":["8513"],"award-info":[{"award-number":["8513"]}],"id":[{"id":"10.13039\/501100004488","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>To date, the microbiome, as well as the virome of the Croatian populations of bats, was unknown. Here, we present the results of the first viral metagenomic analysis of guano, feces and saliva (oral swabs) of seven bat species (Myotis myotis, Miniopterus schreibersii, Rhinolophus ferrumequinum, Eptesicus serotinus, Myotis blythii, Myotis nattereri and Myotis emarginatus) conducted in Mediterranean and continental Croatia. Viral nucleic acids were extracted from sample pools, and analyzed using Illumina sequencing. The presence of 63 different viral families representing all seven Baltimore groups were confirmed, most commonly insect viruses likely reflecting the diet of insectivorous bats. Virome compositions of our samples were largely impacted by the sample type: invertebrate-infecting viruses were most frequently found in feces, bacterial viruses in guano, whereas vertebrate-infecting viruses were most common in swabs. Most vertebrate-infecting virus sequences were assigned to retroviruses, parvoviruses, iridoviruses, and poxviruses. We further report the complete genome sequence of a novel adeno-associated virus, densovirus and a near complete length genome sequence of a novel iflavirus. Additionally, one of the most interesting findings in this study was the difference in viromes between two contrasting habitats, the continental and Mediterranean Croatia.<\/jats:p>","DOI":"10.3390\/v12080891","type":"journal-article","created":{"date-parts":[[2020,8,14]],"date-time":"2020-08-14T13:00:18Z","timestamp":1597410018000},"page":"891","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Viral Metagenomic Profiling of Croatian Bat Population Reveals Sample and Habitat Dependent Diversity"],"prefix":"10.3390","volume":"12","author":[{"given":"Ivana","family":"\u0160imi\u0107","sequence":"first","affiliation":[{"name":"Laboratory for Rabies and General Virology, Department of Virology, Croatian Veterinary Institute, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8191-0994","authenticated-orcid":false,"given":"Toma\u017e Mark","family":"Zorec","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Institute of Microbiology and Immunology, University of Ljubljana, 1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1559-9020","authenticated-orcid":false,"given":"Ivana","family":"Lojki\u0107","sequence":"additional","affiliation":[{"name":"Laboratory for Rabies and General Virology, Department of Virology, Croatian Veterinary Institute, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nina","family":"Kre\u0161i\u0107","sequence":"additional","affiliation":[{"name":"Laboratory for Rabies and General Virology, Department of Virology, Croatian Veterinary Institute, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3216-7564","authenticated-orcid":false,"given":"Mario","family":"Poljak","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Institute of Microbiology and Immunology, University of Ljubljana, 1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2237-1243","authenticated-orcid":false,"given":"Florence","family":"Cliquet","sequence":"additional","affiliation":[{"name":"Nancy Laboratory for Rabies and Wildlife, ANSES, 51220 Malz\u00e9ville, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evelyne","family":"Picard-Meyer","sequence":"additional","affiliation":[{"name":"Nancy Laboratory for Rabies and Wildlife, ANSES, 51220 Malz\u00e9ville, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5054-3634","authenticated-orcid":false,"given":"Marine","family":"Wasniewski","sequence":"additional","affiliation":[{"name":"Nancy Laboratory for Rabies and Wildlife, ANSES, 51220 Malz\u00e9ville, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vida","family":"Zrn\u010di\u0107","sequence":"additional","affiliation":[{"name":"Croatian Biospeleological Society, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"An\u0111ela","family":"\u0106uku\u0161i\u0107","sequence":"additional","affiliation":[{"name":"Croatian Biospeleological Society, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomislav","family":"Bedekovi\u0107","sequence":"additional","affiliation":[{"name":"Laboratory for Rabies and General Virology, Department of Virology, Croatian Veterinary Institute, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,8,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Wang, L.-F., and Cowled, C. 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