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These variants exhibited distinct plaque sizes and virulence in a mouse model. Comparing the full-genome sequences of all variants, several nucleotide changes were identified in different genomic regions. Furthermore, different sequential variants were revealed to co-exist within one sample as quasispecies. Interestingly, the above-mentioned nucleotide changes found within the whole genome sequences of the new variants were present alongside the nucleotide sequence of the parental strain, which was represented as a minority quasispecies. These observations further imply that TBEV exists as a heterogeneous population that contains virus variants pre-adapted to reproduction in different environments, probably enabling virus survival in ticks and mammals.<\/jats:p>","DOI":"10.3390\/v12080902","type":"journal-article","created":{"date-parts":[[2020,8,19]],"date-time":"2020-08-19T09:22:31Z","timestamp":1597828951000},"page":"902","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Tick-Borne Encephalitis Virus Adaptation in Different Host Environments and Existence of Quasispecies"],"prefix":"10.3390","volume":"12","author":[{"given":"Renata","family":"Helmov\u00e1","sequence":"first","affiliation":[{"name":"Faculty of Science, University of South Bohemia in \u010cesk\u00e9 Bud\u011bjovice, 37005 \u010cesk\u00e9 Bud\u011bjovice, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0469-4604","authenticated-orcid":false,"given":"V\u00e1clav","family":"H\u00f6nig","sequence":"additional","affiliation":[{"name":"Faculty of Science, University of South Bohemia in \u010cesk\u00e9 Bud\u011bjovice, 37005 \u010cesk\u00e9 Bud\u011bjovice, Czech Republic"},{"name":"Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, 370 05 \u010cesk\u00e9 Bud\u011bjovice, Czech Republic"},{"name":"Department of Virology, Veterinary Research Institute, 62100 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4132-0372","authenticated-orcid":false,"given":"Hana","family":"Tykalov\u00e1","sequence":"additional","affiliation":[{"name":"Faculty of Science, University of South Bohemia in \u010cesk\u00e9 Bud\u011bjovice, 37005 \u010cesk\u00e9 Bud\u011bjovice, Czech Republic"},{"name":"Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, 370 05 \u010cesk\u00e9 Bud\u011bjovice, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4279-7092","authenticated-orcid":false,"given":"Martin","family":"Palus","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, 370 05 \u010cesk\u00e9 Bud\u011bjovice, Czech Republic"},{"name":"Department of Virology, Veterinary Research Institute, 62100 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7305-0477","authenticated-orcid":false,"given":"Lesley","family":"Bell-Sakyi","sequence":"additional","affiliation":[{"name":"Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Liverpool L3 5RF, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Libor","family":"Grubhoffer","sequence":"additional","affiliation":[{"name":"Faculty of Science, University of South Bohemia in \u010cesk\u00e9 Bud\u011bjovice, 37005 \u010cesk\u00e9 Bud\u011bjovice, Czech Republic"},{"name":"Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, 370 05 \u010cesk\u00e9 Bud\u011bjovice, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,8,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1086\/515195","article-title":"Tick-borne encephalitis","volume":"28","author":"Dumpis","year":"1999","journal-title":"Clin. 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