{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T16:38:38Z","timestamp":1789576718525,"version":"build-2803163510"},"reference-count":50,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2020,8,29]],"date-time":"2020-08-29T00:00:00Z","timestamp":1598659200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005073","name":"Agency for Defense Development","doi-asserted-by":"publisher","award":["UE134020ID"],"award-info":[{"award-number":["UE134020ID"]}],"id":[{"id":"10.13039\/501100005073","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000774","name":"Defense Threat Reduction Agency","doi-asserted-by":"publisher","award":["JSTO-CBD CB4040"],"award-info":[{"award-number":["JSTO-CBD CB4040"]}],"id":[{"id":"10.13039\/100000774","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["HHSN272201800013C"],"award-info":[{"award-number":["HHSN272201800013C"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["HHSN272200700016I"],"award-info":[{"award-number":["HHSN272200700016I"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging human pathogen, endemic in areas of China, Japan, and the Korea (KOR). It is primarily transmitted through infected ticks and can cause a severe hemorrhagic fever disease with case fatality rates as high as 30%. Despite its high virulence and increasing prevalence, molecular and functional studies in situ are scarce due to the limited availability of high-titer SFTSV exposure stocks. During the course of field virologic surveillance in 2017, we detected SFTSV in ticks and in a symptomatic soldier in a KOR Army training area. SFTSV was isolated from the ticks producing a high-titer viral exposure stock. Through the use of advanced genomic tools, we present here a complete, in-depth characterization of this viral stock, including a comparison with both the virus in its arthropod source and in the human case, and an in vivo study of its pathogenicity. Thanks to this detailed characterization, this SFTSV viral exposure stock constitutes a quality biological tool for the study of this viral agent and for the development of medical countermeasures, fulfilling the requirements of the main regulatory agencies.<\/jats:p>","DOI":"10.3390\/v12090958","type":"journal-article","created":{"date-parts":[[2020,8,30]],"date-time":"2020-08-30T06:06:22Z","timestamp":1598767582000},"page":"958","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["A Model for the Production of Regulatory Grade Viral Hemorrhagic Fever Exposure Stocks: From Field Surveillance to Advanced Characterization of SFTSV"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8587-7001","authenticated-orcid":false,"given":"Unai","family":"Perez-Sautu","sequence":"first","affiliation":[{"name":"Center for Genome Sciences, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Fort Detrick, Frederick, MD 21702, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Se Hun","family":"Gu","sequence":"additional","affiliation":[{"name":"The 4th Research &amp; Development Institute, Agency for Defense Development (ADD), Daejeon 34186, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katie","family":"Caviness","sequence":"additional","affiliation":[{"name":"Center for Genome Sciences, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Fort Detrick, Frederick, MD 21702, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong Hyun","family":"Song","sequence":"additional","affiliation":[{"name":"The 4th Research &amp; Development Institute, Agency for Defense Development (ADD), Daejeon 34186, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Jin","family":"Kim","sequence":"additional","affiliation":[{"name":"Army Headquarters, Gyeryong-si 32800, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas Di","family":"Paola","sequence":"additional","affiliation":[{"name":"Center for Genome Sciences, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Fort Detrick, Frederick, MD 21702, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daesang","family":"Lee","sequence":"additional","affiliation":[{"name":"The 4th Research &amp; Development Institute, Agency for Defense Development (ADD), Daejeon 34186, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terry A.","family":"Klein","sequence":"additional","affiliation":[{"name":"Force Health Protection and Preventive Medicine, Medical Department Activity-Korea\/65th Medical Brigade, Unit 15281, APO AP 96271, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph A.","family":"Chitty","sequence":"additional","affiliation":[{"name":"Center for Genome 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at Fort Detrick (IRF-Frederick), Division of Clinical Research (DCR), National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Fort Detrick, Frederick, MD 21702, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7800-6045","authenticated-orcid":false,"given":"Jens H.","family":"Kuhn","sequence":"additional","affiliation":[{"name":"Integrated Research Facility at Fort Detrick (IRF-Frederick), Division of Clinical Research (DCR), National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Fort Detrick, Frederick, MD 21702, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiankun","family":"Zeng","sequence":"additional","affiliation":[{"name":"Pathology Division, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Fort Detrick, Frederick, MD 21702, 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USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo","family":"Palacios","sequence":"additional","affiliation":[{"name":"Center for Genome Sciences, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Fort Detrick, Frederick, MD 21702, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seong Tae","family":"Jeong","sequence":"additional","affiliation":[{"name":"The 4th Research &amp; Development Institute, Agency for Defense Development (ADD), Daejeon 34186, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,8,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Kuhn, J.H., Adkins, S., Alioto, D., Alkhovsky, S.V., Amarasinghe, G., Anthony, S.J., Av\u0161i\u010d-\u017dupanc, T., Ayll\u00f3n, M.A., Bahl, J., and Balkema-Buschmann, A. (2020). 2020 Taxonomic update for phylum Negarnaviricota (Riboviria: Orthornavirae), including the lathe orders Bunyavirales and Mononegavirales. Arch. Virol., in press.","DOI":"10.1007\/s00705-020-04731-2"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1523","DOI":"10.1056\/NEJMoa1010095","article-title":"Fever with thrombocytopenia associated with a novel bunyavirus in China","volume":"364","author":"Yu","year":"2011","journal-title":"N. Engl. J. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"992","DOI":"10.4269\/ajtmh.16-0967","article-title":"The emergence of severe fever with thrombocytopenia syndrome virus","volume":"97","author":"Silvas","year":"2017","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1892","DOI":"10.3201\/eid1911.130792","article-title":"Severe fever with thrombocytopenia syndrome, South Korea, 2012","volume":"19","author":"Kim","year":"2013","journal-title":"Emerg. Infect. Dis."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1735","DOI":"10.1093\/jme\/tjx123","article-title":"Francisella-like endosymbiont detected in Haemaphysalis ticks (Acari: Ixodidae) from the Republic of Korea","volume":"54","author":"Takhampunya","year":"2017","journal-title":"J. Med. Entomol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1080\/22221751.2019.1710436","article-title":"The first discovery of severe fever with thrombocytopenia syndrome virus in Taiwan","volume":"9","author":"Lin","year":"2020","journal-title":"Emerg. Microbes Infect."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1093\/infdis\/jit603","article-title":"The first identification and retrospective study of severe fever with thrombocytopenia syndrome in Japan","volume":"209","author":"Takahashi","year":"2014","journal-title":"J. Infect. Dis."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.3201\/eid2505.181463","article-title":"Endemic severe fever with thrombocytopenia syndrome, Vietnam","volume":"25","author":"Tran","year":"2019","journal-title":"Emerg. Infect. Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"4703","DOI":"10.1007\/s00436-015-4718-2","article-title":"First detection of severe fever with thrombocytopenia syndrome virus in the tick species Haemaphysalis concinna in Shandong Province, China","volume":"114","author":"Meng","year":"2015","journal-title":"Parasitol. Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"970","DOI":"10.1016\/j.ttbdis.2016.05.003","article-title":"Molecular detection of severe fever with thrombocytopenia syndrome and tick-borne encephalitis viruses in ixodid ticks collected from vegetation, Republic of Korea, 2014","volume":"7","author":"Yun","year":"2016","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1093\/cid\/cir804","article-title":"Hemorrhagic fever caused by a novel bunyavirus in China: Pathogenesis and correlates of fatal outcome","volume":"54","author":"Zhang","year":"2012","journal-title":"Clin. Infect. Dis."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"868","DOI":"10.3201\/eid2405.151435","article-title":"Transmission of severe fever with thrombocytopenia syndrome virus by Haemaphysalis longicornis ticks, China","volume":"24","author":"Zhuang","year":"2018","journal-title":"Emerg. Infect. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1093\/cid\/cir776","article-title":"Person-to-person transmission of severe fever with thrombocytopenia syndrome bunyavirus through blood contact","volume":"54","author":"Gai","year":"2012","journal-title":"Clin. Infect. Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"963","DOI":"10.3201\/eid1806.111345","article-title":"Severe fever with thrombocytopenia syndrome virus, Shandong Province, China","volume":"18","author":"Zhao","year":"2012","journal-title":"Emerg. Infect. Dis."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"756","DOI":"10.3201\/eid1905.120245","article-title":"Severe fever with thrombocytopenia syndrome virus among domesticated animals, China","volume":"19","author":"Niu","year":"2013","journal-title":"Emerg. Infect. Dis."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"11990","DOI":"10.1038\/s41598-019-48317-8","article-title":"Severe fever with thrombocytopenia syndrome phlebovirus causes lethal viral hemorrhagic fever in cats","volume":"9","author":"Park","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2126","DOI":"10.3201\/eid2012.141013","article-title":"Prevalence of SFTSV among Asian house shrews and rodents, China, January-August 2013","volume":"20","author":"Liu","year":"2014","journal-title":"Emerg. Infect. Dis."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.ijid.2018.11.017","article-title":"Animals as amplification hosts in the spread of severe fever with thrombocytopenia syndrome virus: A systematic review and meta-analysis","volume":"79","author":"Chen","year":"2019","journal-title":"Int. J. Infect. Dis."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e2533","DOI":"10.1097\/MD.0000000000002533","article-title":"Natural transmission model for severe fever with thrombocytopenia syndrome bunyavirus in villages of Hubei Province, China","volume":"95","author":"Xing","year":"2016","journal-title":"Medicine"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1016\/S1473-3099(14)70718-2","article-title":"Severe fever with thrombocytopenia syndrome, an emerging tick-borne zoonosis","volume":"14","author":"Liu","year":"2014","journal-title":"Lancet Infect. Dis."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/j.ttbdis.2016.12.015","article-title":"Novel sub-lineages, recombinants and reassortants of severe fever with thrombocytopenia syndrome virus","volume":"8","author":"Lv","year":"2017","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"319","DOI":"10.3347\/kjp.2013.51.3.319","article-title":"Seasonal distribution of ticks in four habitats near the demilitarized zone, Gyeonggi-do (Province), Republic of Korea","volume":"51","author":"Chong","year":"2013","journal-title":"Korean J. Parasitol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1603\/ME12032","article-title":"Comparison of dragging and sweeping methods for collecting ticks and determining their seasonal distributions for various habitats, Gyeonggi Province, Republic of Korea","volume":"50","author":"Chong","year":"2013","journal-title":"J. Med. Entomol."},{"key":"ref_24","first-page":"147","article-title":"Tick surveillance in four southwestern provinces of the Republic of Korea during 2013","volume":"21","author":"Coburn","year":"2016","journal-title":"Syst. Appl. Acarol."},{"key":"ref_25","first-page":"271","article-title":"Tick surveillance in two southeastern provinces, including three metropolitan areas, of the Republic of Korea during 2014","volume":"22","author":"Johnson","year":"2017","journal-title":"Syst. Appl. Acarol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.jcv.2011.09.031","article-title":"Early diagnosis of novel SFTS bunyavirus infection by quantitative real-time RT-PCR assay","volume":"53","author":"Sun","year":"2012","journal-title":"J. Clin. Virol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1093\/oxfordjournals.aje.a118408","article-title":"A simple method of estimating fifty per cent endpoints","volume":"27","author":"Reed","year":"1938","journal-title":"Am. J. Epidemiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1038\/s41426-018-0071-8","article-title":"A novel sheet-like virus particle array is a hallmark of Zika virus infection","volume":"7","author":"Liu","year":"2018","journal-title":"Emerg. Microbes Infect."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1083\/jcb.17.1.208","article-title":"The use of lead citrate at high pH as an electron-opaque stain in electron microscopy","volume":"17","author":"Reynolds","year":"1963","journal-title":"J. Cell Biol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2448","DOI":"10.1056\/NEJMoa1509773","article-title":"Molecular evidence of sexual transmission of Ebola virus","volume":"373","author":"Mate","year":"2015","journal-title":"N. Engl. J. Med."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"10","DOI":"10.14806\/ej.17.1.200","article-title":"Cutadapt removes adapter sequences from high-throughput sequencing reads","volume":"17","author":"Martin","year":"2011","journal-title":"EMBnet.journal"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1093\/bioinformatics\/btr026","article-title":"Quality control and preprocessing of metagenomic datasets","volume":"27","author":"Schmieder","year":"2011","journal-title":"Bioinformatics"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1016\/j.chom.2015.11.008","article-title":"Evolution and spread of Ebola virus in Liberia, 2014\u20132015","volume":"18","author":"Ladner","year":"2015","journal-title":"Cell Host Microbe"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.1923","article-title":"Fast gapped-read alignment with Bowtie 2","volume":"9","author":"Langmead","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2078","DOI":"10.1093\/bioinformatics\/btp352","article-title":"1000 Genome Project Data Processing Subgroup, The Sequence Alignment\/Map format and SAMtools","volume":"25","author":"Li","year":"2009","journal-title":"Bioinformatics"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1792","DOI":"10.1093\/nar\/gkh340","article-title":"MUSCLE: Multiple sequence alignment with high accuracy and high throughput","volume":"32","author":"Edgar","year":"2004","journal-title":"Nucleic Acids Res."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Kugelman, J.R., Wiley, M.R., Nagle, E.R., Reyes, D., Pfeffer, B.P., Kuhn, J.H., Sanchez-Lockhart, M., and Palacios, G.F. (2017). Error baseline rates of five sample preparation methods used to characterize RNA virus populations. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0171333"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"e129531","DOI":"10.1172\/jci.insight.129531","article-title":"Genetic and pathogenic diversity of severe fever with thrombocytopenia syndrome virus (SFTSV) in South Korea","volume":"5","author":"Yun","year":"2020","journal-title":"JCI Insight"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1093\/molbev\/mst010","article-title":"MAFFT multiple sequence alignment software version 7: Improvements in performance and usability","volume":"30","author":"Katoh","year":"2013","journal-title":"Mol. Biol. Evol."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Price, M.N., Dehal, P.S., and Arkin, A.P. (2010). FastTree 2\u2014Approximately maximum-likelihood trees for large alignments. PLoS ONE, 5.","DOI":"10.1371\/journal.pone.0009490"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1007\/BF02101694","article-title":"Dating of the human-ape splitting by a molecular clock of mitochondrial DNA","volume":"22","author":"Hasegawa","year":"1985","journal-title":"J. Mol. Evol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1007\/BF02101990","article-title":"A new method for calculating evolutionary substitution rates","volume":"20","author":"Lanave","year":"1984","journal-title":"J. Mol. Evol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1038\/nmeth.2109","article-title":"jModelTest 2: More models, new heuristics and parallel computing","volume":"9","author":"Darriba","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1002\/cjs.5550360302","article-title":"Bayesian analysis of elapsed times in continuous-time Markov chains","volume":"36","author":"Ferreira","year":"2008","journal-title":"Can. J. Stat."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1093\/sysbio\/syy032","article-title":"Posterior summarization in Bayesian phylogenetics using Tracer 1.7","volume":"67","author":"Rambaut","year":"2018","journal-title":"Syst. Biol."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Drummond, A.J., and Rambaut, A. (2007). BEAST: Bayesian evolutionary analysis by sampling trees. BMC Evol. Biol., 7.","DOI":"10.1186\/1471-2148-7-214"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"27","DOI":"10.2220\/biomedres.39.27","article-title":"Targeting of severe fever with thrombocytopenia syndrome virus structural proteins to the ERGIC (endoplasmic reticulum Golgi intermediate compartment) and Golgi complex","volume":"39","author":"Lundu","year":"2018","journal-title":"Biomed. Res."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Plegge, T., Hofmann-Winkler, H., Spiegel, M., and P\u00f6hlmann, S. (2016). Evidence that processing of the severe fever with thrombocytopenia syndrome virus Gn\/Gc polyprotein is critical for viral infectivity and requires an internal Gc signal peptide. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0166013"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"3026","DOI":"10.1128\/JVI.03432-14","article-title":"Reverse genetics system for severe fever with thrombocytopenia syndrome virus","volume":"89","author":"Brennan","year":"2015","journal-title":"J. Virol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"e00061-15","DOI":"10.1128\/mSphere.00061-15","article-title":"Efficacy of T-705 (favipiravir) in the treatment of infections with lethal severe fever with thrombocytopenia syndrome virus","volume":"1","author":"Tani","year":"2016","journal-title":"mSphere"}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/12\/9\/958\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:04:37Z","timestamp":1760177077000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/12\/9\/958"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,29]]},"references-count":50,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2020,9]]}},"alternative-id":["v12090958"],"URL":"https:\/\/doi.org\/10.3390\/v12090958","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8,29]]}}}