{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T14:11:02Z","timestamp":1780582262034,"version":"3.54.1"},"reference-count":55,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2020,9,25]],"date-time":"2020-09-25T00:00:00Z","timestamp":1600992000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010665","name":"H2020 Marie Sk\u0142odowska-Curie Actions","doi-asserted-by":"publisher","award":["721367"],"award-info":[{"award-number":["721367"]}],"id":[{"id":"10.13039\/100010665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Atypical porcine pestivirus (APPV) is a single-stranded RNA virus from the family Flaviviridae, which is linked to congenital tremor (CT) type A-II in newborn piglets. Here, we retrospectively investigated the molecular evolution of APPV on an affected herd between 2013 and 2019. Monitoring was done at regular intervals, and the same genotype of APPV was found during the entire study period, suggesting no introductions from outside the farm. The nucleotide substitutions over time did not show substantial amino acid variation in the structural glycoproteins. Furthermore, the evolution of the virus showed mainly purifying selection, and no positive selection. The limited pressure on the virus to change at immune-dominant regions suggested that the immune pressure at the farm might be low. In conclusion, farms can have circulation of APPV for years, and massive testing and removal of infected animals are not sufficient to clear the virus from affected farms.<\/jats:p>","DOI":"10.3390\/v12101080","type":"journal-article","created":{"date-parts":[[2020,9,27]],"date-time":"2020-09-27T22:24:42Z","timestamp":1601245482000},"page":"1080","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Atypical Porcine Pestivirus Circulation and Molecular Evolution within an Affected Swine Herd"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7688-6288","authenticated-orcid":false,"given":"Alba","family":"Folgueiras-Gonz\u00e1lez","sequence":"first","affiliation":[{"name":"Department Discovery &amp; Technology, MSD Animal Health, Wim de K\u00f6rverstraat 35, P.O. Box 31, 5830AA Boxmeer, The Netherlands"},{"name":"Laboratory of Experimental Virology, Department of Medical Microbiology, Amsterdam UMC Location AMC, University of Amsterdam, Meibergdreef 9, 1105AZ Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7492-0614","authenticated-orcid":false,"given":"Robin","family":"van den Braak","sequence":"additional","affiliation":[{"name":"Department Discovery &amp; Technology, MSD Animal Health, Wim de K\u00f6rverstraat 35, P.O. Box 31, 5830AA Boxmeer, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bartjan","family":"Simmelink","sequence":"additional","affiliation":[{"name":"Department Discovery &amp; Technology, MSD Animal Health, Wim de K\u00f6rverstraat 35, P.O. Box 31, 5830AA Boxmeer, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Deijs","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Virology, Department of Medical Microbiology, Amsterdam UMC Location AMC, University of Amsterdam, Meibergdreef 9, 1105AZ Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2803-642X","authenticated-orcid":false,"given":"Lia","family":"van der Hoek","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Virology, Department of Medical Microbiology, Amsterdam UMC Location AMC, University of Amsterdam, Meibergdreef 9, 1105AZ Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4129-4354","authenticated-orcid":false,"given":"Ad","family":"de Groof","sequence":"additional","affiliation":[{"name":"Department Discovery &amp; Technology, MSD Animal Health, Wim de K\u00f6rverstraat 35, P.O. Box 31, 5830AA Boxmeer, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,9,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/bs.aivir.2017.07.003","article-title":"New Leaves in the Growing Tree of Pestiviruses","volume":"99","author":"Blome","year":"2017","journal-title":"Adv. Virus Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2106","DOI":"10.1099\/jgv.0.000873","article-title":"Proposed revision to the taxonomy of the genus Pestivirus, family Flaviviridae","volume":"98","author":"Smith","year":"2017","journal-title":"J. Gen. 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