{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T10:35:51Z","timestamp":1792060551021,"version":"4.4.0"},"reference-count":41,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2020,9,30]],"date-time":"2020-09-30T00:00:00Z","timestamp":1601424000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R21AI148836"],"award-info":[{"award-number":["R21AI148836"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"name":"School of Medicine Research Allocations Committee, University of New Mexico","award":["Pilot Award"],"award-info":[{"award-number":["Pilot Award"]}]},{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences","doi-asserted-by":"publisher","award":["KL2TR001448"],"award-info":[{"award-number":["KL2TR001448"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences","doi-asserted-by":"publisher","award":["UL1TR001449"],"award-info":[{"award-number":["UL1TR001449"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["K12GM088021"],"award-info":[{"award-number":["K12GM088021"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["P30CA118100"],"award-info":[{"award-number":["P30CA118100"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Identifying the specific epitopes targeted by antibodies elicited in response to infectious diseases is important for developing vaccines and diagnostics. However, techniques for broadly exploring the specificity of antibodies in a rapid manner are lacking, limiting our ability to quickly respond to emerging viruses. We previously reported a technology that couples deep sequencing technology with a bacteriophage MS2 virus-like particle (VLP) peptide display platform for identifying pathogen-specific antibody responses. Here, we describe refinements that expand the number of patient samples that can be processed at one time, increasing the utility of this technology for rapidly responding to emerging infectious diseases. We used dengue virus (DENV) as a model system since much is already known about the antibody response. Sera from primary DENV-infected patients (n = 28) were used to pan an MS2 bacteriophage VLP library displaying all possible 10-amino-acid peptides from the DENV polypeptide. Selected VLPs were identified by deep sequencing and further investigated by enzyme-linked immunosorbent assay. We identified previously described immunodominant regions of envelope and nonstructural protein-1, as well as a number of other epitopes. Our refinement of the deep sequence-coupled biopanning technology expands the utility of this approach for rapidly investigating the specificity of antibody responses to infectious diseases.<\/jats:p>","DOI":"10.3390\/v12101114","type":"journal-article","created":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T09:04:12Z","timestamp":1601543052000},"page":"1114","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Expansion and Refinement of Deep Sequence-Coupled Biopanning Technology for Epitope-Specific Antibody Responses in Human Serum"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2307-5529","authenticated-orcid":false,"given":"Nikole L.","family":"Warner","sequence":"first","affiliation":[{"name":"Department and Molecular Genetics and Microbiology, University of New Mexico Health Sciences, Albuquerque, NM 87131, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0504-0741","authenticated-orcid":false,"given":"Alexandria C.","family":"Linville","sequence":"additional","affiliation":[{"name":"Department and Molecular Genetics and Microbiology, University of New Mexico Health Sciences, Albuquerque, NM 87131, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan B.","family":"Core","sequence":"additional","affiliation":[{"name":"Department and Molecular Genetics and Microbiology, University of New Mexico Health Sciences, Albuquerque, NM 87131, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brechla","family":"Moreno","sequence":"additional","affiliation":[{"name":"Gorgas Memorial Institute, Panama 0801, Panama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan M.","family":"Pascale","sequence":"additional","affiliation":[{"name":"Gorgas Memorial Institute, Panama 0801, Panama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David S.","family":"Peabody","sequence":"additional","affiliation":[{"name":"Department and Molecular Genetics and Microbiology, University of New Mexico Health Sciences, Albuquerque, NM 87131, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bryce","family":"Chackerian","sequence":"additional","affiliation":[{"name":"Department and Molecular Genetics and Microbiology, University of New Mexico Health Sciences, Albuquerque, NM 87131, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathryn M.","family":"Frietze","sequence":"additional","affiliation":[{"name":"Department and Molecular Genetics and Microbiology, University of New Mexico Health Sciences, Albuquerque, NM 87131, USA"},{"name":"Clinical and Translational Science Center, University of New Mexico Health Sciences, Albuquerque, NM 87131, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,9,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1158\/2326-6066.CIR-15-0165","article-title":"Identification of anti-CA125 antibody responses in ovarian cancer patients by a novel deep sequence-coupled biopanning platform","volume":"4","author":"Frietze","year":"2016","journal-title":"Cancer Immunol. 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