{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:00:13Z","timestamp":1790834413299,"version":"4.1.0"},"reference-count":38,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2020,10,12]],"date-time":"2020-10-12T00:00:00Z","timestamp":1602460800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>The surveillance of swine influenza A viruses in France revealed the emergence of an antigenic variant following deletions and mutations that are fixed in the HA-encoding gene of the European human-like reassortant swine H1N2 lineage. In this study, we compared the outcomes of the parental (H1huN2) and variant (H1huN2\u039414\u2013147) virus infections in experimentally-inoculated piglets. Moreover, we assessed and compared the protection that was conferred by an inactivated vaccine currently licensed in Europe. Three groups of five unvaccinated or vaccinated piglets were inoculated with H1huN2 or H1huN2\u039414\u2013147 or mock-inoculated, respectively. In unvaccinated piglets, the variant strain induced greater clinical signs than the parental virus, in relation to a higher inflammatory response that involves TNF-\u03b1 production and a huge afflux of granulocytes into the lung. However, both infections led to similar levels of virus excretion and adaptive (humoral and cellular) immune responses in blood. The vaccinated animals were clinically protected from both infectious challenges and did not exhibit any inflammatory responses, regardless the inoculated virus. However, whereas vaccination prevented virus shedding in H1huN2-infected animals, it did not completely inhibit the multiplication of the variant strain, since live virus particles were detected in nasal secretions that were taken from H1huN2\u039414\u2013147-inoculated vaccinated piglets. This difference in the level of vaccine protection was probably related to the poorer ability of the post-vaccine antibodies to neutralize the variant virus than the parental virus, even though post-vaccine cellular immunity appeared to be equally effective against both viruses. These results suggest that vaccine antigens would potentially need to be updated if this variant becomes established in Europe.<\/jats:p>","DOI":"10.3390\/v12101155","type":"journal-article","created":{"date-parts":[[2020,10,13]],"date-time":"2020-10-13T21:48:38Z","timestamp":1602625718000},"page":"1155","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Evaluation of the Pathogenicity and the Escape from Vaccine Protection of a New Antigenic Variant Derived from the European Human-Like Reassortant Swine H1N2 Influenza Virus"],"prefix":"10.3390","volume":"12","author":[{"given":"C\u00e9line","family":"Deblanc","sequence":"first","affiliation":[{"name":"Swine Virology Immunology Unit, Ploufragan-Plouzan\u00e9-Niort Laboratory, French Agency for Food, Environmental and Occupational Health and Safety (ANSES), 22440 Ploufragan, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phane","family":"Qu\u00e9guiner","sequence":"additional","affiliation":[{"name":"Swine Virology Immunology Unit, Ploufragan-Plouzan\u00e9-Niort Laboratory, French Agency for Food, Environmental and Occupational Health and Safety (ANSES), 22440 Ploufragan, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phane","family":"Gorin","sequence":"additional","affiliation":[{"name":"Swine Virology Immunology Unit, Ploufragan-Plouzan\u00e9-Niort Laboratory, French Agency for Food, Environmental and Occupational Health and Safety (ANSES), 22440 Ploufragan, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Am\u00e9lie","family":"Chastagner","sequence":"additional","affiliation":[{"name":"Swine Virology Immunology Unit, Ploufragan-Plouzan\u00e9-Niort Laboratory, French Agency for Food, Environmental and Occupational Health and Safety (ANSES), 22440 Ploufragan, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2223-7071","authenticated-orcid":false,"given":"S\u00e9verine","family":"Herv\u00e9","sequence":"additional","affiliation":[{"name":"Swine Virology Immunology Unit, Ploufragan-Plouzan\u00e9-Niort Laboratory, French Agency for Food, Environmental and Occupational Health and Safety (ANSES), 22440 Ploufragan, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fr\u00e9d\u00e9ric","family":"Paboeuf","sequence":"additional","affiliation":[{"name":"SPF Pig Production and Experimentation, Ploufragan-Plouzan\u00e9-Niort Laboratory, French Agency for Food, Environmental and Occupational Health and Safety (ANSES), 22440 Ploufragan, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1099-5075","authenticated-orcid":false,"given":"Ga\u00eblle","family":"Simon","sequence":"additional","affiliation":[{"name":"Swine Virology Immunology Unit, Ploufragan-Plouzan\u00e9-Niort Laboratory, French Agency for Food, Environmental and Occupational Health and Safety (ANSES), 22440 Ploufragan, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,10,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Simon, G., Larsen, L.E., Durrwald, R., Foni, E., Harder, T., Van Reeth, K., Markowska-Daniel, I., Reid, S.M., Dan, A., and Maldonado, J. 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