{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T12:55:22Z","timestamp":1787316922237,"version":"build-2736575974"},"reference-count":39,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2020,12,25]],"date-time":"2020-12-25T00:00:00Z","timestamp":1608854400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>In rabies diagnosis, it is essential to count on a rapid test to give a quick response. The combined sensitivity and robustness of the TaqMan RT-PCR assays (qRT-PCR) have made these methods a valuable alternative for rabies virus (RABV) detection. We conducted a study to compare the applicability of two widely used qRT-PCR assays targeting the nucleoprotein gene (LysGT1 assay) and leader sequences (LN34 qRT-PCR assay) of RABV genomes, in all variants circulating in Argentina. A total of 44 samples obtained from bats, dogs, cattle, and horses, that were previously tested for rabies by FAT and conventional RT-PCR, were used in the study. All variants were successfully detected by the pan-lyssavirus LN34 qRT-PCR assay. The LysGT1 assay failed to detect three bat-related variants. We further sequenced the region targeted by LysGT1 and demonstrated that the presence of three or more mismatches with respect to the primers and probe sequences precludes viral detection. We conclude that the LysGT1 assay is prone to yield variant-dependent false-negative test results, and in consequence, the LN34 assay would ensure more effective detection of RABV in Argentina.<\/jats:p>","DOI":"10.3390\/v13010023","type":"journal-article","created":{"date-parts":[[2020,12,25]],"date-time":"2020-12-25T09:30:19Z","timestamp":1608888619000},"page":"23","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Evaluation of Two Real-Time, TaqMan Reverse Transcription-PCR Assays for Detection of Rabies Virus in Circulating Variants from Argentina: Influence of Sequence Variation"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0345-7861","authenticated-orcid":false,"given":"Diego A.","family":"Caraballo","sequence":"first","affiliation":[{"name":"Instituto de Zoonosis \u201cLuis Pasteur\u201d, Av. D\u00edaz V\u00e9lez 4821, Ciudad Aut\u00f3noma de Buenos Aires C1405DCD, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda A.","family":"Lombardo","sequence":"additional","affiliation":[{"name":"Instituto de Zoonosis \u201cLuis Pasteur\u201d, Av. D\u00edaz V\u00e9lez 4821, Ciudad Aut\u00f3noma de Buenos Aires C1405DCD, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula","family":"Becker","sequence":"additional","affiliation":[{"name":"Instituto de Zoonosis \u201cLuis Pasteur\u201d, Av. D\u00edaz V\u00e9lez 4821, Ciudad Aut\u00f3noma de Buenos Aires C1405DCD, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda S.","family":"Sabio","sequence":"additional","affiliation":[{"name":"Servicio de Neurovirosis, Instituto Nacional de Enfermedades Infecciosas, Administraci\u00f3n Nacional de Laboratorios e Institutos de Salud (ANLIS), \u201cDr. Carlos G. Malbr\u00e1n\u201d, Av. V\u00e9lez Sarsfield 563, Ciudad Aut\u00f3noma de Buenos Aires C1282AFF, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Lema","sequence":"additional","affiliation":[{"name":"Servicio de Neurovirosis, Instituto Nacional de Enfermedades Infecciosas, Administraci\u00f3n Nacional de Laboratorios e Institutos de Salud (ANLIS), \u201cDr. Carlos G. Malbr\u00e1n\u201d, Av. V\u00e9lez Sarsfield 563, Ciudad Aut\u00f3noma de Buenos Aires C1282AFF, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leila M.","family":"Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Servicio de Neurovirosis, Instituto Nacional de Enfermedades Infecciosas, Administraci\u00f3n Nacional de Laboratorios e Institutos de Salud (ANLIS), \u201cDr. Carlos G. Malbr\u00e1n\u201d, Av. V\u00e9lez Sarsfield 563, Ciudad Aut\u00f3noma de Buenos Aires C1282AFF, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando J.","family":"Beltr\u00e1n","sequence":"additional","affiliation":[{"name":"Instituto de Zoonosis \u201cLuis Pasteur\u201d, Av. D\u00edaz V\u00e9lez 4821, Ciudad Aut\u00f3noma de Buenos Aires C1405DCD, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Li","sequence":"additional","affiliation":[{"name":"Poxvirus and Rabies Branch, Division of High Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30329, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7614-9272","authenticated-orcid":false,"given":"Daniel M.","family":"Cisterna","sequence":"additional","affiliation":[{"name":"Servicio de Neurovirosis, Instituto Nacional de Enfermedades Infecciosas, Administraci\u00f3n Nacional de Laboratorios e Institutos de Salud (ANLIS), \u201cDr. Carlos G. Malbr\u00e1n\u201d, Av. V\u00e9lez Sarsfield 563, Ciudad Aut\u00f3noma de Buenos Aires C1282AFF, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,12,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2417","DOI":"10.1007\/s00705-019-04306-w","article-title":"Changes to virus taxonomy and the International Code of Virus Classification and Nomenclature ratified by the International Committee on Taxonomy of Viruses (2019)","volume":"164","author":"Walker","year":"2019","journal-title":"Arch. 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